LOCUS       sequence-341703-        9135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax 
            (PIP3 marker and opto-PI3K).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Kakumoto T, Nakata T
  TITLE     Optogenetic control of PIP3: PIP3 is sufficient to induce the 
            actin-based active part of growth cones and is regulated via 
            endocytosis.
  JOURNAL   PLoS One. 2013 Aug 7;8(8):e70861. doi: 10.1371/journal.pone.0070861.
            eCollection 2013.
  PUBMED    23951027
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-7"; volume: "8(8):e70861. doi"; pages: " 
            10.1371/journal.pone.0070861. eCollection 2013"
COMMENT     Sequence Label: AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
FEATURES             Location/Qualifiers
     source          1..9135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          599..1615
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1539..1561
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1623..1642
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2231..2240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2237..2944
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2382..2400)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2650..2669
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2945..2998
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3011..4504
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             5138..5191
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5204..5713
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     polyA_signal    5897..6018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5934..5953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5988..6007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6025..6480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6162..6183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6374..6393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6507..6611
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6613..6970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6613..6804
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6636..6656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6821..6956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7005..7799
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7005..7799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7059..7078)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7669..7688
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7987..8006)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8031..8078
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8407..8995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8896..8915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(9121..9135,1..365)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
       61 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      121 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      181 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      241 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      301 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      361 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 gggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      541 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      601 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      661 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      721 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      781 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      841 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      901 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
      961 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1021 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1081 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1141 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1201 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1261 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1321 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1381 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1441 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1501 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1561 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1621 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattga tttatcgcta
     1681 gcgctaccgg actcagatct cgagctcaag cttcgaattc gccaccatga gcgacgtggc
     1741 tattgtgaag gagggttggc tgcacaaacg aggggagtac atcaagacct ggcggccacg
     1801 ctacttcctc ctcaagaatg atggcacctt cattggctac aaggagcgtc cgcaggatgt
     1861 ggaccaacgt gaggctcccc tcaacaactt ctctgtggcg cagtgccagc tgatgaagac
     1921 ggagcggccc cggcccaaca ccttcatcat ccgctgcctg cagtggacca ctgtcatcga
     1981 acgcaccttc catgtggaga ctcctgagga gcgggaggag tggacaaccg ccatccagac
     2041 tgtggctgac ggcctcaaga agcaggagga ggaggagatg gacttccggt cgggctcacc
     2101 cagtgacaac tcaggggctg aagagatgga ggtgtccctg gccaagccca agcaccgcgt
     2161 gaccatgaac gagtttgagt acctgaagct gctgggcaag ggcactttcg gcaaggtgga
     2221 tccaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     2281 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     2341 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2401 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     2461 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     2521 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     2581 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     2641 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     2701 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     2761 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     2821 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     2881 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     2941 caaggagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggccccgc
     3001 accgggtacc atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat
     3061 tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat
     3121 ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa
     3181 acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat
     3241 caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa
     3301 agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga
     3361 gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc
     3421 gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa
     3481 atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat
     3541 aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga
     3601 ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc
     3661 tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa
     3721 gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag
     3781 cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa
     3841 cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc
     3901 tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg
     3961 gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg
     4021 ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag
     4081 aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg
     4141 aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg
     4201 gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc
     4261 gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct
     4321 tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa
     4381 agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc
     4441 tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc
     4501 agcaagtgct ggaggtagtg ctggaggttc agctggtgga cctagagcat ccaagtacca
     4561 acaagaccag gtggtgaagg aggacagcgt agaggctgtg ggcgcccagc tcaaggtcta
     4621 ccaccagcag taccaggaca agagccgcga atatgaccag ctgtatgaag aatacacacg
     4681 gacctcccag gagctgcaga tgaagcgcac agccatagag gccttcaacg agaccatcaa
     4741 gatcttcgaa gagcagggcc agacacagga gaagtgcagc aaggagtatt tggagcgctt
     4801 ccggcgagag ggaaatgaga aggagatgca gaggatcctg ctgaactccg agcgactcaa
     4861 gtctcgcatc gcggagatac acgaaagccg cacgaagttg gagcaggatc tgcgggcgca
     4921 ggcctccgac aaccgtgaga tcgacaagcg catgaacagc ctcaaacctg acctcatgca
     4981 gctgcgcaag atcagggacc agtacctcgt gtggctcacc cagaaaggtg cccgacagag
     5041 gaagatcaac gaatggctgg gaatcaagaa cgagactgag gaccagtatt cactgatgga
     5101 ggatgaggac gccctccccc accacgagga gcgcacggag ggccgcggaa gtcttctaac
     5161 atgcggggac gtggaggaaa atcccggccc cgcaccggga tccatgaatg gagctatagg
     5221 aggtgacctt ttgctcaatt ttcctgacat gtcggtccta gagcgccaaa gggctcacct
     5281 caagtacctc aatcccacct ttgattctcc tctcgccggc ttctttgccg attcttcaat
     5341 gattaccggc ggcgagatgg acagctatct ttcgactgcc ggtttgaatc ttccgatgat
     5401 gtacggtgag acgacggtgg aaggtgattc aagactctca atttcgccgg aaacgacgct
     5461 tgggactgga aatttcaagg cagcgaagtt tgatacagag actaaggatt gtaatgaggc
     5521 ggcgaagaag atgacgatga acagagatga cctagtagaa gaaggagaag aagagaagtc
     5581 gaaaataaca gagcaaaaca atgggagcac aaaaagcatc aagaagatga aacacaaagc
     5641 caagaaagaa gagaacaatt tctctaatga ttcatctaaa gtgacgaagg aattggagaa
     5701 aacggattat attcatgtac tgtataaggg taaaaagaag aaaaagaagt caaagacaaa
     5761 gtgtgtaatt atgtagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     5821 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5881 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6001 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     6061 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6121 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6181 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6241 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6301 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6361 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6421 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6481 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6541 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6601 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     6661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6721 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6781 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6901 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6961 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     7021 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7081 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7141 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     7201 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7261 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7321 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7381 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7441 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7501 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     7561 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7621 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7681 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7741 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7801 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7861 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7921 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     7981 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     8041 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     8101 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     8161 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     8221 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     8281 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8341 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8401 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8461 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8521 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8581 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8641 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8701 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8761 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8821 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8881 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8941 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9001 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9061 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattatc
     9121 gacattgatt attga
//
