LOCUS       sequence-341736-       10440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT 
            fusion driven by CAG promoter and tagBFP driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Kong X, Wang Z, Zhang R, Wang X, Zhou Y, Shi L, Yang H
  TITLE     Precise genome editing without exogenous donor DNA via retron 
            editing system in human cells.
  JOURNAL   Protein Cell. 2021 Aug 17. pii: 10.1007/s13238-021-00862-7. doi: 
            10.1007/s13238-021-00862-7.
  PUBMED    34403072
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Cell. 2021 Aug 17. pii: 10.1007/s13238-021-00862-7. doi: 
            10.1007/s13238-021-00862-7."
COMMENT     Sequence Label: pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        683..960
                     /label=chicken beta-actin promoter
     intron          961..1189
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1224..1233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1236..1256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1281..5381
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1281..5381
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6468..6488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6501..6518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6507..6714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7079..7113
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        7171..7474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7475..7678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7629..7649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7707..8408
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    8409..8616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8643..8747
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8748..8816,8817..9608)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8966..8985)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9779..10367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10268..10287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      841 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     1021 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     1081 tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg ttggtggggt
     1141 attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg ttggaccggt
     1201 gggtaccgaa gccgctagtc gacgccacca tggccccaaa gaagaagcgg aaggtcggta
     1261 tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc ggcaccaact
     1321 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     1381 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     1441 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     1501 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     1561 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     1621 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     1681 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     1741 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1801 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1861 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1921 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1981 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     2041 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     2101 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     2161 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     2221 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     2281 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     2341 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     2401 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     2461 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     2521 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     2581 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     2641 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     2701 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     2761 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2821 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2881 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2941 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     3001 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     3061 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     3121 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     3181 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     3241 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     3301 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     3361 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     3421 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     3481 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     3541 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     3601 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     3661 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     3721 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3781 actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3841 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3901 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3961 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     4021 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     4081 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     4141 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     4201 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     4261 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     4321 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     4381 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     4441 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     4501 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     4561 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     4621 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     4681 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     4741 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4801 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4861 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4921 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4981 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     5041 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     5101 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     5161 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     5221 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     5281 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     5341 tgtacgagac acggatcgac ctgtctcagc tgggaggcga ctccggagga tctagcggag
     5401 gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa agcagcgggg
     5461 gcagcagcgg ggggtcaagc cggatctaca gcctgattga tagccagacg ctgatgacca
     5521 agggctttgc cagcgaggtg atgagaagcc ctgaacctcc caagaagtgg gacatcgcca
     5581 agaaaaaggg cggaatgagg accatctacc accctagctc taaggttaag ctgatccaat
     5641 actggctgat gaacaacgtg ttcagcaagt tgcctatgca caatgccgcc tacgcttttg
     5701 tgaaaaaccg gtccatcaag tctaacgccc tgctgcacgc cgagagcaag aacaagtact
     5761 acgtgaagat cgacctgaag gatttcttcc ccagcatcaa gttcaccgac ttcgagtacg
     5821 ccttcacaag ataccgggac agaatcgagt tcaccaccga gtacgacaaa gaactgctgc
     5881 agctgatcaa gaccatctgc ttcatcagcg attccaccct gcctatcggc ttccctacat
     5941 ctccactgat cgctaatttc gtcgccagag agctggatga aaagctgacc cagaagctga
     6001 acgccatcga caagctgaac gctacataca ccagatacgc cgacgacatc atcgtgtcca
     6061 ccaacatgaa gggcgccagc aaactgatcc tggactgctt caaaagaaca atgaaggaaa
     6121 tcggacctga tttcaagatt aacattaaga aattcaagat ctgtagcgcc tctggcggca
     6181 gcatcgtggt gaccggcctg aaagtgtgcc acgattttca catcacactg cacagatcca
     6241 tgaaggataa gatccggctg catctgtctc tgctgagcaa gggcatcctg aaggacgagg
     6301 accacaacaa gctcagcggc tacatcgcct atgctaagga catcgacccc cacttctaca
     6361 caaagcttaa tagaaagtat tttcaggaga tcaaatggat ccagaacctg cacaacaaag
     6421 tggaatctgg cggctcaaaa agaaccgccg acggcagcga gttcgagccc aagaagaaga
     6481 ggaaagtcta aaagcttcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6541 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6601 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6661 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc
     6721 gcgtcgacgg cgcgccctag acgaagcagc tccagcctac acaatcgctc aagacgtgta
     6781 atgcttttat tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt
     6841 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     6901 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     6961 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     7021 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     7081 tgcgtcaatt ttacgcagac tatctttcta gggttaaggc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctctaga cgttacataa cttacggtaa atggcccgcc
     7201 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     7261 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     7321 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     7381 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     7441 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     7501 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     7561 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     7621 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     7681 tttagtgaac cgtcagatcc gctagcatga gcgagctgat taaggagaac atgcacatga
     7741 agctgtatat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag
     7801 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     7861 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc
     7921 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga
     7981 gagtcaccac atacgaggac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     8041 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg
     8101 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacactgtac cccgctgacg
     8161 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg
     8221 caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg
     8281 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acatacgtcg
     8341 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc
     8401 tgaattaact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8461 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8521 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8581 ggaggattgg gaagagaata gcaggcatgc tggggaggat ccgcactttt cggggaaatg
     8641 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8701 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8761 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8821 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8881 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8941 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9001 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9061 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9121 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9181 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9241 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9301 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9361 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9421 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9481 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9541 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9601 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9661 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9721 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9781 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9841 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9901 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9961 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10021 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10081 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10141 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10201 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10261 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10321 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10381 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
//
