LOCUS       sequence-341759-        5611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase enhancer reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Long HK, Osterwalder M, Welsh IC, Hansen K, Davies JOJ, Liu YE, 
            Koska M, Adams AT, Aho R, Arora N, Ikeda K, Williams RM, 
            Sauka-Spengler T, Porteus MH, Mohun T, Dickel DE, Swigut T, Hughes 
            JR, Higgs DR, Visel A, Selleri L, Wysocka J
  TITLE     Loss of Extreme Long-Range Enhancers in Human Neural Crest Drives a 
            Craniofacial Disorder.
  JOURNAL   Cell Stem Cell. 2020 Nov 5;27(5):765-783.e14. doi: 
            10.1016/j.stem.2020.09.001. Epub 2020 Sep 28.
  PUBMED    32991838
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2020.09.001"; journalName: "Cell Stem Cell"; date: 
            "2020-11-5- 5"; volume: "27"; issue: "5"; pages: "765-783.e14"
COMMENT     Sequence Label: pGL3-noSV40-humanEC1.45_p300peak2
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..208
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      59..194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..1896
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(310..328)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1937..2058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1948..1967)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2002..2021
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2312..2329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2483..3071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2563..2582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3242..4033,4034..4102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3865..3884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4103..4207)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4234..4689
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4321..4340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4531..4552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4820..4868
                     /note="synthetic polyadenylation signal"
     misc_feature    4882..4973
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4922..4941
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
       61 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt
      121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      181 cttttttgga ggcctaggct tttgcaaaaa gcttggcatt ccggtactgt tggtaaagcc
      241 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
      301 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      361 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
      421 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
      481 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
      541 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
      601 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
      661 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
      721 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
      781 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
      841 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
      901 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
      961 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     1021 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     1081 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     1141 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     1201 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     1261 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     1321 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     1381 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     1441 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     1501 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     1561 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     1621 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     1681 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     1741 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     1801 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     1861 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg
     1921 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1981 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2041 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2101 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     2161 gatctggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     2221 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     2281 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4201 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     4261 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4321 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4381 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4441 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4501 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4561 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4621 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4681 ttacaatttg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4741 cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg
     4801 aggtacttgg agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     4861 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     4921 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
     4981 ggtaccgagc tcttaagaga agcccgagtg agtaaccaac tgtccccaat tgtattagcc
     5041 ttttaaataa aggattccca gcaaacattc agaagaaacc atatgccttc cagggatagg
     5101 cagctggaag agagggaaga gattaataca gaaaaaagct ataggccatc ccgatcctat
     5161 tcttcagttc ctttattgga ggcattaagt tttattttat taacttcttt cttgcccttc
     5221 aaaagttgag ggtgctcact ccagggcttg tggatatctg ccccagagaa cctcagtgca
     5281 aaggtttgaa atcacagctg gctgtgatta gaaggattgg gagagatcag tgcttgatta
     5341 actttcagct gctattggtt tgcctcagtt cctgcaggag aattaatctg taggttcgag
     5401 taaattttgg atcttaatgg ataccagata cattgagccc acccagacca ccaagatttt
     5461 gcaatccttt tcctcacatt taaatggtac aaatgaattc attcctcctt actcagtgaa
     5521 cagaataact ctacctcaag gtttttctca tatagcattc acttgatttt gccttctttt
     5581 ccaagaaagt atagattcgt tggggcaagg a
//
