LOCUS       sequence-341770-        6049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ArpC1 subunit of the Human Arp2/3 complex in a library vector for 
            the biGBac system of insect expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, 
            Dominguez R
  TITLE     Cryo-EM structure of NPF-bound human Arp2/3 complex and activation 
            mechanism.
  JOURNAL   Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaaz7651. doi: 
            10.1126/sciadv.aaz7651. Print 2020 Jun.
  PUBMED    32917641
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-06-5"; volume: "6(23). pii: 6/23/eaaz7651. doi"; pages: 
            " 10.1126/sciadv.aaz7651. Print 2020 Jun"
COMMENT     Sequence Label: ArpC1_pLib
FEATURES             Location/Qualifiers
     source          1..6049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      115..569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(202..221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     411..432
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        595..699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(700..768,769..1560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(918..937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1731..2319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2220..2239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2473..2490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2624..2848
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2915..3448)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3637..3642,3643..3659,3660..3665))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(4061..4094)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4142..4233
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4158..4175
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     RBS             4829..4837
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5571..5705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5608..5627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5662..5681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5815..5980
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
       61 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
      121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt
      421 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
      481 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      541 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
      601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1861 cagataccaa atactattct tctagtgtag ccgtagttag gccaccactt caagaactct
     1921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2461 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     2521 ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa
     2581 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     2641 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     2701 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     2761 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     2821 ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     2881 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     2941 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     3001 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     3061 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     3121 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     3181 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
     3241 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
     3301 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     3361 atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     3421 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     3481 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     3541 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     3601 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     3661 gtcaactggg tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     3721 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     3781 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     3841 ctgcccttgc ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     3901 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     3961 caggactcta gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt
     4021 gaactgctga tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca
     4081 ttatacgaag ttatctggtt ttactagtat cgattcgcga cctactccgg aatattaata
     4141 gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     4201 cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc
     4261 atcgggcgcg gatctgcggc cgcgaattcc ttgtccccgc tcgcggatcc atggcctatc
     4321 actccttctt ggtagaacct ataagctgtc acgcatggaa caaagacagg acgcaaatag
     4381 caatatgccc gaacaaccat gaagtccaca tttacgagaa aagtggagct aagtggacta
     4441 aggtccatga actgaaggag cacaatggcc aagtaacggg catcgactgg gccccagaaa
     4501 gtaacagaat cgtgacctgc ggtactgacc gtaatgcgta cgtttggacc ttgaaaggaa
     4561 gaacatggaa acctacgctg gtaattctca ggattaatcg tgcggcgaga tgtgtccgtt
     4621 gggctccgaa tgagaacaaa tttgctgtag gtagcggaag tcgtgtgatt agtatctgct
     4681 acttcgaaca agagaacgac tggtgggttt gtaaacacat taaaaaacca atcagatcca
     4741 cggtcctttc actggattgg catcctaata acgtactcct ggcggcgggt tcctgtgatt
     4801 ttaagtgccg catcttctcc gcctatatta aggaggtgga agaaagacca gccccaactc
     4861 catggggcag taagatgcct ttcggcgaac tcatgttcga atctagttct tcttgcggat
     4921 gggttcatgg cgtttgtttt tctgcaagcg gtagtcgcgt ggcttgggtt agccacgaca
     4981 gcacggtttg cctggcagac gcagataaaa agatggctgt agcaaccctt gcgagcgaga
     5041 ctttgccact cttggcactc accttcatca ctgacaactc gctcgttgcg gcaggccatg
     5101 attgctttcc ggtactgttt acttacgacg ccgcagctgg tatgttgtct ttcggcggtc
     5161 gccttgacgt ccccaagcag agctctcagc gcggtctcac tgcccgtgaa agattccaaa
     5221 atcttgataa gaaggcatcc tccgaaggag gtacagccgc gggcgctgga ttggacagtc
     5281 tgcacaaaaa ctcggtgtcc caaatatcgg tattgtccgg aggaaaagct aaatgcagtc
     5341 agttctgcac aacgggaatg gatggaggta tgagcatttg ggacgtcaaa agcctcgaat
     5401 cagccctcaa agatttgaaa attaaataaa ctagtggtac caagcttgtc gagaagtact
     5461 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     5521 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5581 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5641 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5701 ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct
     5761 gagttggctg ctgccaccgc tgagcaataa ctatcataac ccctaggaga tccgaaccag
     5821 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     5881 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     5941 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     6001 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcat
//
