LOCUS       sequence-341780-       17667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express VPS13D lacking N-terminal 117 aa in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17667)
  AUTHORS   Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, 
            Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, 
            Prinz WA, Youle RJ
  TITLE     VPS13D promotes peroxisome biogenesis.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 
            10.1083/jcb.202001188.
  PUBMED    33891012
REFERENCE   2  (bases 1 to 17667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212018. doi"; 
            pages: " 10.1083/jcb.202001188"
COMMENT     Sequence Label: pGFP-Ub-VPS13D_dN
FEATURES             Location/Qualifiers
     source          1..17667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1369..1593
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    14455..14576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14492..14511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14546..14565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(14583..15038)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14720..14741)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14932..14951
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        15065..15169
                     /gene="bla"
                     /label=AmpR promoter
     promoter        15171..15528
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        15171..15362
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(15194..15214)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      15379..15514
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             15563..16357
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             15563..16357
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15617..15636)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16227..16246
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(16545..16564)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    16589..16636
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      16965..17553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17454..17473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcgg gccgcctgca gatcttcgtg
     1381 aagactctga ctggtaagac catcaccctc gaggttgagc ccagtgacac cattgagaat
     1441 gtcaaggcaa agatccaaga taaggaaggc atccctcctg accagcagag gctgatcttt
     1501 gctggaaaac agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc
     1561 accctgcacc tggtactccg tctcagaggt gggatggagg agaaatggaa gaatgaccgc
     1621 cagcagaaag gggagtccta ttggtattca gttaccgcct ccgtagttac aaggattgtg
     1681 gagaatattg aattaaaaat tcaagatgtc catttacgct ttgaagatgg tgtcaccaat
     1741 ccctcccatc cttttgcttt tggcatctgc attaagaatg tgtccatgca aaatgctgtg
     1801 aatgagcctg tacagaaact aatgcggaaa aagcaattag acgtagcaga atttagcatc
     1861 tattgggatg tcgattgcac tttactgggg gatttgcctc agatggagtt acaggaggcc
     1921 atggccagga gcatggagag tcgcagccat cactacgtcc tggagcctgt gtttgcatct
     1981 gctcttttga agagaaactg ctccaagaag cccctgcggt ctcggcacag tccccgtatt
     2041 gattgtgata ttcagctgga gaccattccc ttgaaactct ctcagctgca ataccggcaa
     2101 atcatggaat tcctcaagga gctggaacga aaggagaggc aggtgaagtt ccgaaggtgg
     2161 aaacccaagg tggcgatatc taagaactgc cgagaatggt ggtattttgc tttgaatgct
     2221 aacttgtatg agatcagaga gcagaggaaa cgttgcacct gggactttat gttgcaccgc
     2281 gctcgtgatg ctgtatctta cactgacaaa tatttcaaca agttaaaagg aggcctgctg
     2341 tccacagatg acaaggagga aatgtgtcgg attgaagagg aacagagctt tgaggaattg
     2401 aagattttgc gtgaactggt tcatgatcga tttcacaaac aggaagaact agcagagagt
     2461 ctgcgggagc ctcagtttga ttctccagga gcctgtccgg gagccccaga acccggtgga
     2521 ggcagtggga tgctgcagta tctccagtcc tggtttcctg gatggggtgg ctggtacggg
     2581 cagcagaccc cagaagggaa tgtggttgag ggactgtcag cagagcaaca ggagcagtgg
     2641 attcctgaag agatcctggg cactgaggag ttttttgacc ccactgcaga tgcctcgtgt
     2701 atgaacacgt atacaaagcg agatcatgtc tttgccaaac tgaatttgca gttgcagcga
     2761 ggtacagtga ctctgttaca caaggagcaa ggaactcctc aaatgaatga aagtgctttc
     2821 atgcagctcg agttttcaga tgtaaaactt ctagcagagt ctcttcctcg aagaaattcc
     2881 tcgttgcttt cagtccggtt gggtggactg tttcttcgag acctggctac agaaggaact
     2941 atgtttcctc ttctagtctt ccctaatcca caaaaagaag ttggcagagt ctcacaatct
     3001 tttggtctac aaactacatc tgcagacaga agtgatcatt acccagctgc agatccagat
     3061 ggccccgttt ttgagatgct gtatgagaga aatccggcgc acagccactt tgagaggcgg
     3121 ctcaatgtca gcacaaggcc cttgaacatc atatacaatc cgcaggccat taaaaaagta
     3181 gcagactttt tctacaaggg aaaggttcat acctcaggtt ttggttatca gtctgaactt
     3241 gagctgagag tggctgaagc tgcccgaaga caatataaca agctgaagat gcagaccaag
     3301 gcagaaatcc ggcaaactct tgatcgtttg ctagtgggtg atttcattga ggagagtaaa
     3361 cgatggaccg tgcggctgga tatttctgcc cctcaggtga tatttcctga tgatttcaaa
     3421 ttcaagaatc ctgtgttagt tgtcgtggat ctaggaagaa tgcttttgac gaacacccaa
     3481 gataactcca ggaggaaaag tagggatggg tcagcatctg aagagaccca gtttagtgat
     3541 gatgaatata agacccccct ggccacacct cctaacaccc cacctcccga gtcaagcagc
     3601 agcaacggag agaaaacacc tcccttttct ggagttgagt tcagtgaaga acagcttcaa
     3661 gcacatttaa tgagcacaaa gatgtatgag aggtactcgc tgtcatttat ggacctccag
     3721 atcatggttg gacgagtgaa agacaattgg aagcatgtcc aggatattga cgtgggacca
     3781 acacatgtgg tagagaagtt caacgttcac ctacagttag agcgtcgatt gatttatact
     3841 tcagatccca aatatccagg agccgtgctc tcaggcaact taccagactt aaaaatccac
     3901 attaatgaag ataaaatatc tgcactaaag aattgctttg ctctcctcac caccccagaa
     3961 atgaaaactt ctgacactca gattaaagaa aagatttttc cccaggagga gcagcgggga
     4021 agtttgcaag actccgtaat gaatttaacc cagagcattg tgttgttgga gcagcatacc
     4081 cgcgaggttc tggtggagtc gcagctcctc ctggcggaat ttaaagtgaa ctgtatgcag
     4141 cttggtgttg agagcaatgg ccggtacatt tctgtgctca aggtgtttgg taccaatgct
     4201 cactttgtga agaggcctta tgatgctgaa gtctccctaa ctgttcatgg tttgctcctg
     4261 gtggatacca tgcagacata tggtgctgat tttgaccttt tgatggcttc acataagaac
     4321 ttgagctttg atattccaac gggaagcctt cgggatagca gggcccagtc tcctgtctct
     4381 ggaccgaatg tggcccactt aactgatgga gctacactga acgaccgatc agctactagt
     4441 gtttcacttg acaaaattct taccaaagag caagagtccc ttattaagtt ggaatatcag
     4501 tttgtgagtt cagagtgccc atcgatgaat ttagacagta ctcttcaggt gatttcccta
     4561 caggtgaata atttagatat tatcctcaat ccagagacga ttgtggagct aattggtttt
     4621 cttcaaaaat cctttcccaa ggaaaaagat gatttaagtc ctcaaccttt aatgactgat
     4681 tttgaaagaa gcttcagaga acaaggaact taccagtcta catatgaaca aaacactgag
     4741 gttgcagtgg aaatccatag gctgaactta ctgcttcttc ggacagtggg catggcaaat
     4801 agagagaaat atggcagaaa aattgcaact gcaagtatag gtggcaccaa agttaatgtc
     4861 tcaatgggta gcacgtttga catgaatggt tctcttggct gtttacagct tatggatttg
     4921 acacaagata acgttaaaaa ccagtatgtt gtcagcattg ggaattctgt aggctatgaa
     4981 aatatcatca gtgatattgg ctactttgaa tctgtgtttg tcagaatgga agatgcagcc
     5041 ctcactgaag ctttgagttt cacgtttgtt gagagatcta aacaggagtg ttttctcaac
     5101 ctgaagatgg cttctttaca ttataaccac tctgctaagt ttttgaagga gttgacgtta
     5161 tccatggatg aactggaaga aaattttcga ggtatgctga aaagcgcagc caccaaagtc
     5221 accacagtac tagctaccaa gactgccgag tatagcgaga tggtatcgct ctttgaaact
     5281 ccaaggaaga ctcgggaacc ctttatctta gaggaaaatg aaatatatgg gtttgaccta
     5341 gcttcgtctc atttggacac tgtaaagcta atcttgaaca taaacattga atcaccagtt
     5401 gtttctatcc ctcggaagcc ggggagtcct gagttgttgg tgggacactt gggacagata
     5461 ttcatccaga attttgtggc gggagatgat gaatccagaa gtgaccgtct gcaggtggaa
     5521 atcaaggaca ttaaactgta ttctttgaat tgcacccagt tggcaggtag agaagctgtt
     5581 gggtctgaag gaagccggat gttttgccca ccttccgggt ctggcagtgc caacagtcag
     5641 gaggaagctc atttcacacg acatgatttc tttgaatctt tgcatagagg tcaagctttt
     5701 cacatcctga acaacaccac cattcagttt aaactggaga agatccctat agagagagaa
     5761 tctgaattga ctttttctct tagcccagat gacctgggaa cttctagcat catgaagatt
     5821 gaaggaaaat ttgtcaatcc agttcaggtg gtgttagcaa agcatgtata tgagcaggtt
     5881 ttacaaaccc tggacaatct cgtgtacagt gaagatctga ataagtatcc agccagtgct
     5941 acctcctccc cttgccctga ttctcctctg cctcccctca gtacctgtgg agaatcttct
     6001 gttgaaagga aggagaatgg attgttcagc cactccagcc tttctaacac ctctcagaag
     6061 tcattgtcag tgaaggaagt caaatccttt actcagattc aagccacctt ttgtatatca
     6121 gagcttcagg ttcagctaag tggagatctg actttggggg cccaaggtct tgtgagctta
     6181 aagtttcagg actttgaggt ggaattcagt aaagaccatc cccagacttt atctattcag
     6241 attgccctgc attctctgct gatggaggac ttattggaga agaatccaga ttctaaatat
     6301 aagaacctga tggtgtctcg aggagcccct aagccatcta gtttagcaca aaaagaatac
     6361 ctttctcagt cttgcccctc agtgtccaat gtggaatatc ctgatatgcc tcggtctctc
     6421 ccttcccaca tggaagaagc tcctaatgtc ttccagttgt atcaaaggcc cacctctgcg
     6481 tcccggaaaa agcaaaagga agtccaagac aaggactatc ccttgacccc acctccttct
     6541 ccaacagtgg atgagcccaa gatacttgtt ggaaagagta aatttgatga ttccttagtc
     6601 cacatcaaca tattcttggt agataagaaa catccagaat tctcttccag ttacaatcga
     6661 gttaaccgga gcattgatgt tgattttaat tgcttggatg tgctgatcac actgcaaacc
     6721 tgggttgtga tattagactt ttttggaatc ggctccactg cagacaacca cgcaatgagg
     6781 ctgcctcctg agggcattct gcacaacgtg aagttggagc cacatgcctc catggagtct
     6841 ggacttcagg atccagtgaa caccaaactg gatctcaagg ttcattcact ttctctagtg
     6901 ctgaataaga ccaccagtga gcttgccaaa gcaaatgtgt ccaaattagt agcacacctg
     6961 gaaatgattg agggagacct ggccttacag ggcagcattg ggagtctgtc tctaagtgac
     7021 ctcacatgcc atggagagtt ctacagagaa cggttcacta ccagtggtga agaagcactc
     7081 atcttccaga cttttaaata cggacggcct gaccctctgc tccggagaga acacgacatt
     7141 cgcgtgagcc tccggatggc ctctgtgcag tatgtgcata ctcagcgttt ccaggcagag
     7201 gtggtggcct tcattcagca tttcactcag ctgcaggatg tcttagggcg ccagcgagct
     7261 gctattgagg ggcagacggt gagagatcaa gcccagcgct gttcacgggt tctcctggat
     7321 attgaggctg gtgctcccgt tctcttgatc ccagaaagtt ccagatcaaa taatctgatt
     7381 gtagcaaatt tggggaagtt gaaagtcaaa aataagtttc tgtttgctgg ttttcctggc
     7441 accttttccc tacaagataa ggaatctgtg ccttcagctt ccccaacggg tattcccaaa
     7501 cacagtctga ggaaaacgac aagcacggag gagcccaggg gaacccattc ccaggggcag
     7561 ttcacgatgc ctcttgctgg aatgagccta ggaagcctga agagtgagtt tgtgcccagt
     7621 acctccacca agcagcaagg gccgcaaccc acactgtctg ttggccaaga gtccagtagt
     7681 ccagaagacc atgtctgcct gctggattgc gttgtcgtgg atctccagga catggacatc
     7741 tttgctgcag agagacatcc gagagaatac tcgaaggcac cagaggatag tagtggagat
     7801 ctgatcttcc cttcctattt tgtgcgacag acaggaggaa gcctcttaac cgagccttgt
     7861 aggctgaaat tgcaggtgga aaggaatttg gacaaagaaa taagtcatac tgtgccagac
     7921 atatctatcc atggcaatct ctcctcagtc cactgctctc tggatctgta taaatacaag
     7981 ctgatccgcg gcttattaga gaacaacctg ggagaaccca tagaggaatt tatgcggcct
     8041 tatgatttac aagatccaag aattcatact gtcctgagtg gagaagtgta cacctgtatg
     8101 tgcttcctca ttgatatggt gaatgtaagt ctggagctta aagatccaaa aagaaaagaa
     8161 ggtgctgggt ccctagccag atttgacttc aagaaatgca aactgctcta tgaaagtttt
     8221 tccaaccaaa ccaagtccat taacttggtt tcccattcca tgatggcttt tgacacccgt
     8281 tatgctgggc agaagaccag ccctggcatg acgaatgtgt tcagctgtat ctttcagccc
     8341 gctaagaaca gcagcaccac ccaagggtcc attcagattg aactacattt cagatctacc
     8401 aaggattcct cctgctttac agtagttctc aacaatctcc gtgtgtttct catatttgac
     8461 tggctactgt tagtccatga ttttctccac actcccagtg atattaagaa acaaaatcat
     8521 gttactcctt ctcgccaccg taactctagc agcgaatctg ctatagttcc caaaactgtg
     8581 aagagtggag tagttaccaa gcggtcttcc cttcctgtgt ccaatgaaag gcacctggag
     8641 gtcaaggtca atgtaacagg tacggagttt gtggtcattg aagatgtgtc ctgcttcgac
     8701 accaatgcca ttattctgaa aggcaccaca gtgctcacct ataagccccg gtttgttgat
     8761 cgcccctttt caggaagttt gtttggcatt gaggtgtttt catgccgact agggaatgag
     8821 catgatacag ctctttcaat tgtggatccc gtacaaattc aaatggagtt ggtggggaat
     8881 tcttcttatc aaaatagttc aggattgatg gatgcattca atagtgaaga tttcccacct
     8941 gtcctggaga ttcagttaca agccctggat atcagactct cctataatga tgttcagctg
     9001 tttcttgcca ttgcaaaatc catcccagag caagctaatg ctgcagtgcc agactcagtg
     9061 gccctggagt cagactccgt tggcacttac cttccaggtg catctcgcgt tggagaggaa
     9121 atcagagaag ggacaagaca caccttagat cctgtcttgg agttacagct ggctaggctg
     9181 caggagctgg gattcagcat ggatgattgt cgcaaagctc ttttggcgtg tcaaggccaa
     9241 ttgaaaaagg cagcaagttg gttgtttaag aatgcggaac ctctgaagtc tctttccttg
     9301 gcctccacca gccgagatag cccaggggct gtggcagcgc cattgatctc tggcgtggag
     9361 atcaaagctg agagtgtgtg catctgtttc atcgatgact gcatggattg tgatgttcct
     9421 ctcgctgaac tcaccttttc ccgtctgaat tttcttcagc gtgtaagaac tagccctgaa
     9481 ggctatgccc acttcaccct ttctggagat tattataacc gtgctctttc aggctgggag
     9541 ccatttattg agccttggcc atgctctgta tcctggcaac agcaggcagc tagtcgtctc
     9601 catcctcctc gactgaagct agaagccaag gccaaacctc gtttggatat caatatcact
     9661 tctgtgctaa ttgaccagta tgtaagtacc aaggaatcgt ggatggcaga ttactgtaaa
     9721 gatgacaagg acatagagtc agctaaatca gaagactgga tgggctcttc ggtggatcct
     9781 ccatgttttg gacaaacaga ggtgaaaacc cccaagcgcc ggcagccatt tgtccccttt
     9841 gctctgagga accacacggg gtgcactttg tggtttgcca ccctgaccac cacacccacc
     9901 agagctgcac tctctcacag tgggagtcca ggggtagttc cagaagggaa cggaacattt
     9961 ctcgatgata ctcacaatgt tagtgaatgg cgagaagtcc ttacaggtga agagattccc
    10021 tttgaatttg aagcaagagg aaagttaaga cacagacaca cccatgacct ccggattcat
    10081 caactgcaag tgagagtaaa tggctgggag caagtgagcc cagtgtctgt ggacaaagtc
    10141 gggacctttt ttcgatatgc agcaccagat aaaaattcat cttcctctac gattggcagc
    10201 ccaagcagca gaacaaatat tatacatccc caggtttatt tctcttcact cccaccagtg
    10261 cgggtggtct ttgcagtgac tatggaaggc agtgcacgga aagtcatcac tgtccggtca
    10321 gccctcattg tgaggaacag acttgagaca ccaatggaac taagactgga tagcccatca
    10381 gctccagaca agccagtggt gcttcctgct atcatgccag gggattcgtt tgctgtgcct
    10441 ttacacctca cttcttggcg gctacaggcc cggcccaaag gattgggtgt atttttctgt
    10501 aaggctccca ttcattggac caatgtagtg aagactgcag aaattagtag cagtaaacga
    10561 gagtgccact ctatggacac agaaaaaagc cgatttttca ggttttgtgt ggctataaag
    10621 aaagagaatt atccagatta tatgccctca aacatatttt ctgacagtgc aaaacagatt
    10681 ttcagacagc ctgggcatac catatatctc ctgccaactg tggtaatctg caacttgcta
    10741 ccctgtgaac ttgattttta tgttaaagga atgccaatta atgggacgct gaaacctggc
    10801 aaggaggcag ctctccatac agctgataca tcccagaaca ttgagctggg ggtatcactg
    10861 gagaatttcc ccctctgtaa agaattgctc attccacctg gaacccaaaa ctatatggtg
    10921 agaatgcgac tctatgacgt caaccgtcgg cagctgaacc tcaccatccg gattgtgtgt
    10981 cgagcagaag gatccttaaa gatcttcatt tctgctccat attggctgat taacaaaaca
    11041 gggttgccac tgatcttcag acaggacaat gccaagacag atgctgcagg ccagtttgag
    11101 gagcatgagc tggcccgtag cctgagtcct ctcttattct gctatgctga caaagagcag
    11161 ccaaacctct gcacgatgag aatcggaagg gggattcatc cagaaggcat gccgggctgg
    11221 tgtcagggct tctccctgga tggtggtagt ggtgtccgag ctttgaaagt catccagcaa
    11281 ggaaaccgcc cagggctgat ctataacatt ggtattgatg tcaagaaagg ccgaggtcga
    11341 tacattgata cctgcatggt catctttgcc ccccgttacc tgttagataa taaatcatct
    11401 cacaagcttg catttgcaca gagggaattt gccaggggac agggaacagc caatcccgaa
    11461 ggttacattt ccacccttcc tggttccagt gtggtgttcc actggcctcg gaatgactat
    11521 gatcagctat tgtgtgtcag actgatggac gttcccaatt gtatttggtc tggaggcttt
    11581 gaagtcaaca agaataattc cttccatatc aacatgaggg ataccttggg aaaatgcttc
    11641 ttcctacgag tggaaattac tctccgagga gctacgtata ggatctcatt tagtgacaca
    11701 gatcagttac ctcctccttt ccgaattgac aacttttcta aggtcccggt tgtctttact
    11761 cagcatggcg tagctgaacc caggctccgg actgaagtga agcccatgac ttcattggat
    11821 tatgcctggg acgaacccac cttgccacct tttatcactc tgactgttaa aggggcaggg
    11881 tcctctgaga tcaactgcaa catgaatgat ttccaggata atcggcagct ttattatgaa
    11941 aatttcattt acattgctgc tacatataca ttctctggct tgcaggaggg aacaggcagg
    12001 cctgtggctt ccaacaaggc cattacctgt gcggagctcg ttttggatgt ctcacccaag
    12061 acacaaagag tcattttaaa aaagaaggaa ccaggaaagc gttctcagct gtggaggatg
    12121 acaggaacag gaatgctggc ccatgagggc tcctcagttc ctcacaatcc caataagccc
    12181 tcagccgccc gctccaccga ggggtctgcc atcttagata ttgctggtct cgctgcagtg
    12241 actgacaaca gatacgagcc actgatgctg agaaagcctg accgcaggcg aagcacaact
    12301 cagacgtgga gtttccgaga aggaaaactg acctgtgggt tacatgggtt ggtcgtccag
    12361 gccaaaggag gactttctgg tttgtttgat ggagctgaag ttgttcttgg tcctgacact
    12421 tccatggagc ttttggggcc agttccacct gaacaacaat ttattaatca aaaaatgaga
    12481 cctggttctg gaatgttatc catcagagtc atcccagatg gaccaactag agcactccag
    12541 ataacagatt tctgccaccg gaaaagcagc cgttcatatg aagtggatga acttcctgtc
    12601 accgaacaag agctgcagaa attaaagaat ccagatacag agcaggaatt ggaagtgctt
    12661 gtgaggttag aaggtggaat tgggttgtcc ttaattaata aagtcccaga agaactggtc
    12721 tttgcaagtc ttacaggaat caatgtgcac tatacacagc tggcaaccag tcacatgctt
    12781 gaactcagca tacaggatgt acaggtggac aatcagctca ttggtaccac gcagcccttc
    12841 atgctctatg tgactcccct gagcaatgag aatgaggtca tcgagaccgg cccagctgtg
    12901 caagtcaacg cagtgaagtt ccccagtaag agtgcactga ccaacatcta caagcatctg
    12961 atgatcacag ctcagagatt cacagtgcaa attgaggaga aactgctcct caagctgcta
    13021 agtttctttg gctacgatca agcagaatca gaggtggaaa aatatgatga aaacctccat
    13081 gaaaagacag ctgagcaagg tggaacacca attcgatact actttgaaaa tctcaaaatc
    13141 agcattcctc agatcaagct aagtgtgttc acctccaaca agctcccatt ggatcttaag
    13201 gccctaaaaa gcaccttggg gtttcctttg atacggtttg aagacgctgt gattaatcta
    13261 gatccattca ctcgggtaca tccctatgag accaaggagt tcatcatcaa tgatatcctc
    13321 aaacatttcc aggaggaact cctcagccag gcagctcgaa tcctgggatc agtggatttt
    13381 cttggcaatc ctatggggct tttgaatgat gtttctgaag gggttactgg actgataaaa
    13441 tatggaaatg tcgggggcct catcagaaat gttacacacg gagtatcaaa ctctgctgcc
    13501 aagtttgctg gaacattatc agatggctta gggaagacga tggacaatcg gcatcagtca
    13561 gagcgggagt acatcaggta ccatgcagcc acaagtggtg aacaccttgt agccggcatc
    13621 catggcctgg ctcatggtat cattggtgga ctgaccagtg ttataacttc gacagtggaa
    13681 ggtgtgaaaa cagaaggggg tgtcagcggt ttcatatctg gccttggaaa agggcttgtt
    13741 ggcactgtaa ccaagccagt ggcaggcgcc ctggattttg catcagaaac agcccaggcg
    13801 gtgagagaca cagccacact cagcggcccc aggactcaag cacagagggt tcggaaaccg
    13861 cgttgctgca cggggcccca ggggctgctt ccccgatatt ctgagagcca ggcggaagga
    13921 caggagcagc tcttcaaact cacagacaac atacaggacg aattcttcat cgctgtggag
    13981 aacattgaca gctactgcgt gctcatctcc tccaaagctg tttacttcct gaaaagtgga
    14041 gactacgtgg atcgagaagc cattttccta gaagtcaaat acgatgacct ctaccactgc
    14101 cttgtctcca aagaccatgg gaaggtgtat gtgcaggtga ccaagaaagc cgtgagcacg
    14161 agcagtggag tgtccatccc cggcccctcc caccagaagc ccatggtcca tgtgaaatct
    14221 gaggtccttg ctgtcaagtt gtcacaagaa ataaactacg caaagagcct ctactatgaa
    14281 cagcagctta tgttaagact cagcgaaaac cgagagcagc tggagctgga ctcctgatct
    14341 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    14401 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    14461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    14521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
    14581 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    14641 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
    14701 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    14761 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    14821 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    14881 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    14941 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    15001 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
    15061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    15121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
    15181 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    15241 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    15301 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    15361 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    15421 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    15481 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
    15541 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
    15601 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    15661 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    15721 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
    15781 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    15841 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    15901 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    15961 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    16021 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    16081 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    16141 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    16201 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    16261 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    16321 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    16381 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    16441 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    16501 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
    16561 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
    16621 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    16681 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    16741 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    16801 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    16861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    16921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    16981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    17041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    17101 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    17161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    17221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    17281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    17341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    17401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    17461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    17521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    17581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    17641 gtggataacc gtattaccgc catgcat
//
