LOCUS       sequence-341796-       17207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of the 7-subunit Human Arp2/3 complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17207)
  AUTHORS   Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, 
            Dominguez R
  TITLE     Cryo-EM structure of NPF-bound human Arp2/3 complex and activation 
            mechanism.
  JOURNAL   Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaaz7651. doi: 
            10.1126/sciadv.aaz7651. Print 2020 Jun.
  PUBMED    32917641
REFERENCE   2  (bases 1 to 17207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-06-5"; volume: "6(23). pii: 6/23/eaaz7651. doi"; pages: 
            " 10.1126/sciadv.aaz7651. Print 2020 Jun"
COMMENT     Sequence Label: Arp2/3_pBIG2abcd
FEATURES             Location/Qualifiers
     source          1..17207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..405
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             406..1065
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(472..491)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1365..1819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1452..1471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1661..1682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1845..1949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1950..2018,2019..2810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2168..2187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3723..3740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3874..4098
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4165..4698)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4887..4892,4893..4909,4910..4915))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5311..5344)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5442..5490
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5585..5676
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7080..7214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7370..7461
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8902..9036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9395..9443
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9538..9629
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    10751..10885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11041..11132
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11753..11773
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             11780..11803
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    11936..12070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    12429..12477
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        12572..12663
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    13389..13523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13679..13770
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    14445..14579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14938..14986
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        15081..15172
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             15768..15776
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    16510..16644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  16960..17125
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      121 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      181 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      241 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      301 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      361 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      421 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      481 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      541 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      601 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      661 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      721 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      781 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      841 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      901 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      961 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1021 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1081 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     1141 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag
     1201 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt
     1261 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg
     1321 atatgatcat ttattctgcc tcccagagcc tgatggcgaa tgggacgcgc cctgtagcgg
     1381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1561 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1621 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     1681 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1741 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1801 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactattct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3721 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3781 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     3841 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     3901 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     3961 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     4021 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     4081 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4141 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4201 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4261 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     4321 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     4381 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     4441 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     4501 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     4561 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4621 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4681 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4741 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     4801 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     4861 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     4921 tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     4981 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     5041 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
     5101 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     5161 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     5221 gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga
     5281 tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag
     5341 ttatctggcc taggttaatt aaacgctcta tggtctaaag tttttatcga ggctaactga
     5401 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5461 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5521 actctgtcaa gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta
     5581 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     5641 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     5701 accatcgggc gcggatctgc ggccgcgaat tccttgtccc cgctcgcgga tccatggatt
     5761 cgcagggccg caaagtcgtc gtatgcgaca atggtacagg ttttgtgaag tgtggatatg
     5821 ctggaagtaa ctttccagag catatattcc cagcattggt gggacgtcca ataatacgct
     5881 ctactacgaa ggtaggtaac attgaaatta aagaccttat ggttggtgac gaggcatcgg
     5941 agttgaggtc catgcttgag gttaactacc ccatggagaa cggcatagta cgtaactggg
     6001 atgacatgaa acatttgtgg gattacacat ttggacccga aaagctgaat atagacacga
     6061 gaaattgcaa gatcctcctt actgaaccgc ccatgaatcc tactaaaaac cgcgagaaaa
     6121 tcgtagaggt tatgtttgaa acctaccaat tcagcggtgt ttatgtagct atacaggccg
     6181 ttctcacgtt gtacgcacag ggtttgctga ctggtgtcgt ggttgattct ggagatggtg
     6241 tcacacatat ctgtcctgtg tatgagggct tttcacttcc gcacttgacg agacgtcttg
     6301 atattgcagg tcgtgatata acgaggtatt tgatcaagct gctgcttctc aggggatacg
     6361 cattcaatca cagcgcggat ttcgaaaccg taagaatgat taaagaaaag ctgtgttatg
     6421 tcggttacaa catagaacag gaacaaaagc tggctcttga aactactgtt ttggtagagt
     6481 cttacaccct tcctgatgga aggattatca aagttggtgg tgaacgcttc gaagccccgg
     6541 aagcgttgtt ccaaccgcat cttatcaacg tggaaggagt tggcgtagct gagctgctgt
     6601 ttaacacgat tcaagcagcg gatatagaca ctcgtagcga gttctacaaa catatagtcc
     6661 tttcgggagg cagcaccatg tatccgggtc tcccatctcg tcttgaacgt gagttgaagc
     6721 aactgtatct ggaacgtgtc cttaagggag acgtcgaaaa actgtcgaag ttcaagatta
     6781 ggattgaaga tccaccaaga aggaagcata tggtcttcct cggcggagct gtattggctg
     6841 atataatgaa agataaagac aatttctgga tgacaaggca ggaataccag gagaagggag
     6901 ttcgcgtact cgaaaaactc ggtgttactg taagataaac tagtggtacc aagcttgtcg
     6961 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     7021 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     7081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7201 atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga
     7261 aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc atttaaataa
     7321 actggatact attgcacgtt taaatcgacc tactccggaa tattaataga tcatggagat
     7381 aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt
     7441 gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga
     7501 tccatggcgg gacgcttgcc ggcctgcgta gttgactgcg gcacgggata taccaaactc
     7561 ggctatgcgg gcaatacgga accccaattt attataccat cctgtatcgc gataaaggaa
     7621 tccgctaagg taggcgacca ggcgcaaagg cgtgttatga agggtgttga tgacctggac
     7681 tttttcattg gtgacgaagc aatcgaaaaa cctacctacg cgactaagtg gcctatcagg
     7741 catggaatag tagaagattg ggaccttatg gagcgcttta tggaacaagt tatattcaaa
     7801 tacctccgtg cggaacctga agaccattac ttcttgctca ctgaacctcc attgaacaca
     7861 ccggagaacc gcgagtatac tgctgaaata atgttcgaat ctttcaacgt accgggactt
     7921 tacatagctg tacaggcggt gctcgctctt gctgcttctt ggacgtcccg tcaagttggt
     7981 gaacgcaccc tgactggaac agtaatagat agcggcgatg gagtgaccca cgtcattccc
     8041 gttgcggagg gttatgttat aggttcttgc atcaagcaca tacctatagc aggacgcgac
     8101 atcacctact ttatacaaca gcttctcagg gatagggagg tgggaatacc ccctgaacaa
     8161 tcgttggaga cagccaaggc tgtaaaagag agatactcct acgtctgccc cgacctcgta
     8221 aaggagttta ataaatacga cactgacggt agtaagtgga tcaagcaata tacgggtata
     8281 aacgccatca gcaagaaaga atttagtatc gacgtaggat atgaaaggtt tcttggtcct
     8341 gaaatatttt ttcatcctga atttgcgaat ccagatttca cacagccaat ctctgaggta
     8401 gttgacgaag ttatacaaaa ttgtcccatt gatgtaagaa ggccattgta taaaaatatc
     8461 gttctttcgg gtggctcgac tatgttccgt gattttggcc gtagacttca gcgtgatttg
     8521 aaacgcactg ttgacgcgag gctcaagctg tccgaagaac tgtctggcgg tcgcctgaaa
     8581 ccaaagccca tcgatgtgca ggttatcaca caccacatgc agagatacgc agtctggttt
     8641 ggtggatcta tgctggcctc gactccggag ttttatcagg tgtgccacac gaagaaagat
     8701 tacgaggaga tcggcccaag tatttgtcgt cataatcccg tgtttggcgt catgtcctaa
     8761 actagtggta ccaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     8821 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     8881 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     8941 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9001 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
     9061 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     9121 actatcataa ccatttaaat aaatctatat ctcaatcggg gttacgtcga taccccaccg
     9181 agaccccatt gggaccaata cgcccgcgtt tcttcctttt ccccacccca acccccaagt
     9241 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caagccctgc catagccact
     9301 acgggttaat taaaaactat atctcaatcg gggtttttat cgaggctaac tgaaacacgg
     9361 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     9421 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     9481 caagtgaacg ctctatggtc taaagattta aatcgaccta ctccggaata ttaatagatc
     9541 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     9601 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
     9661 ggcgcggatc tgcggccgcg aattccttgt ccccgctcgc ggatccatga ttctgcttga
     9721 ggtcaataat agaataattg aagagactct ggcacttaaa ttcgagaatg cggcggcagg
     9781 aaataagccc gaagccgtag aagttacatt cgctgacttc gatggagtcc tctaccatat
     9841 cagcaacccg aatggtgaca agaccaaagt tatggttagc atcagcctca agttttacaa
     9901 agaactgcaa gctcacggcg cggatgagtt gctcaaacgt gtctatggtt cctttcttgt
     9961 gaatccagaa agcggatata atgtgtcttt gctgtatgat cttgaaaacc tcccggcatc
    10021 aaaggactcc atcgtgcatc aggcgggcat gctgaaaagg aactgttttg cctctgtctt
    10081 cgaaaaatat ttccaatttc aagaggaagg caaggaggga gagaataggg cagttatcca
    10141 ttacagggat gatgagacca tgtatgtaga aagtaaaaag gaccgtgtta ctgttgtttt
    10201 ctcgactgtc tttaaagatg acgatgatgt ggtgataggc aaagtcttta tgcaagagtt
    10261 caaggagggt agaagggcat cgcataccgc tccccaggta ttgttttcgc atagagaacc
    10321 accgcttgag cttaaagata cagatgcggc agtcggagat aacatcggat acattacctt
    10381 cgtactcttt cccagacaca ccaatgcaag cgcgagggat aacactatta acctcatcca
    10441 caccttcagg gattatttgc attaccatat caaatgctct aaagcgtata tccatacccg
    10501 catgagggct aaaacatcag acttccttaa agttcttaat cgtgccagac cagacgcaga
    10561 gaaaaaagaa atgaagacga ttaccggcaa aactttctcc tcacgctaaa ctagtggtac
    10621 caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
    10681 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    10741 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10861 caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta
    10921 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac
    10981 catttaaata aactggatac tattgcacgt ttaaatcgac ctactccgga atattaatag
    11041 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
    11101 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
    11161 tcgggcgcgg atctgcggcc gcgaattcct tgtccccgct cgcggatcca tgcccgcata
    11221 ccactcttca ctcatggacc cggataccaa gctgatcggc aatatggcac ttctgccgat
    11281 tcgctcgcag tttaagggtc cagctccaag agaaaccaaa gacacggaca tcgttgacga
    11341 agccatctat tatttcaagg cgaacgtctt tttcaagaat tatgaaatta agaacgaagc
    11401 ggacagaacc ttgatttata tcactcttta cattagcgaa tgcctcaaga agctccagaa
    11461 gtgcaatagt aaaagccaag gcgaaaagga gatgtacaca ctcggcatta ccaatttccc
    11521 catcccaggc gagcccggtt tccccttgaa tgctatatat gcaaaaccag cgaacaagca
    11581 agaggacgag gtcatgcgcg cctatcttca acagctccgt caagagacag gtttgagact
    11641 gtgcgagaag gttttcgatc cccagaatga taaaccttct aagtggtgga cttgtttcgt
    11701 aaaaagacaa tttatgaata aaagtttgtc cggccccggc cagggaacta gtgagaatct
    11761 ctacttccag ggtggaggag actataaaga cgacgatgac aagtaaaagc ttgtcgagaa
    11821 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    11881 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    11941 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    12001 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12061 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
    12121 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccattt aaataaatct
    12181 atatctcaat cggggttacg tcgatacccc accgagaccc cattgggacc aatacgcccg
    12241 cgtttcttcc ttttccccac cccaaccccc aagttcgggt gaaggcccag ggctcgcagc
    12301 caacgtcggg gcggcaagcc ctgccatagc cactacgggt taattaaaaa ctggatacta
    12361 ttgcacgttt ttatcgaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    12421 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
    12481 aacgcggggt tcggtcccag ggctggcact ctgtcaagtg aacgctctat ggtctaaaga
    12541 tttaaatcga cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat
    12601 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
    12661 aatattccgg attattcata ccgtcccacc atcgggcgcg gatctgcggc cgcgaattcc
    12721 ttgtccccgc tcgcggatcc atgacagcga ccctcaggcc atatctgtct gcggttagag
    12781 caacgctgca agccgcgctc tgtttggaaa actttagcag tcaggtggtt gaacgtcata
    12841 acaagccaga agtcgaggtt cgcagtagta aagagttgct gttgcagcca gtcacgatta
    12901 gccgcaacga aaaagagaag gtgctgattg aaggctcaat caactctgtg cgtgtgtcca
    12961 tcgctgtgaa acaggcggac gagatcgaga agatcctgtg tcacaagttt atgcgcttta
    13021 tgatgatgcg tgctgagaat ttcttcatcc tgagacgcaa acccgtggag ggctatgata
    13081 tctcgtttct tatcacaaat tttcatacgg aacagatgta caaacataaa cttgtagact
    13141 tcgttatcca cttcatggaa gagatcgaca aggagatttc tgaaatgaag ctgagcgtca
    13201 atgcgagggc tcgcatagtt gccgaagagt tcctgaaaaa tttttaaact agtggtacca
    13261 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
    13321 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    13381 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    13441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    13501 atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac
    13561 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact atcataacca
    13621 tttaaataaa ctggatacta ttgcacgttt aaatcgacct actccggaat attaatagat
    13681 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
    13741 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
    13801 gggcgcggat ctgcggccgc gaattccttg tccccgctcg cggatccatg tcaaaaaaca
    13861 ccgtgtcctc tgcccgcttc aggaaggttg atgtagatga gtacgacgag aataagtttg
    13921 ttgacgaaga agacggcggt gacggacagg ctggtccaga tgagggtgag gtggattctt
    13981 gtctccgtca aggtaacatg actgccgcgc ttcaggccgc gttgaaaaac cctcctataa
    14041 acacaaagtc ccaggctgtg aaagatagag ctggttcaat agtcctgaag gtccttatat
    14101 cctttaaggc taacgacata gaaaaggccg tacagtcgct ggacaagaac ggagtggatc
    14161 tcctgatgaa atatatatac aaaggatttg aatcgccctc agataactct tcggcgatgc
    14221 tgttgcaatg gcatgaaaag gcactcgccg caggtggcgt aggctccatt gtccgcgttc
    14281 ttactgcgag aaaaaccgta taaactagtg gtaccaagct tgtcgagaag tactagagga
    14341 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    14401 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    14461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    14521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
    14581 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
    14641 gctgctgcca ccgctgagca ataactatca taaccattta aataaatcta tatctcaatc
    14701 ggggttacgt cgatacccca ccgagacccc attgggacca atacgcccgc gtttcttcct
    14761 tttccccacc ccaaccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
    14821 cggcaagccc tgccatagcc actacgggtt aattaaaaac ctaatgatgc ctgatgtttt
    14881 tatcgaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
    14941 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
    15001 cggtcccagg gctggcactc tgtcaagtga acgctctatg gtctaaagat ttaaatcgac
    15061 ctactccgga atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa
    15121 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga
    15181 ttattcatac cgtcccacca tcgggcgcgg atctgcggcc gcgaattcct tgtccccgct
    15241 cgcggatcca tggcctatca ctccttcttg gtagaaccta taagctgtca cgcatggaac
    15301 aaagacagga cgcaaatagc aatatgcccg aacaaccatg aagtccacat ttacgagaaa
    15361 agtggagcta agtggactaa ggtccatgaa ctgaaggagc acaatggcca agtaacgggc
    15421 atcgactggg ccccagaaag taacagaatc gtgacctgcg gtactgaccg taatgcgtac
    15481 gtttggacct tgaaaggaag aacatggaaa cctacgctgg taattctcag gattaatcgt
    15541 gcggcgagat gtgtccgttg ggctccgaat gagaacaaat ttgctgtagg tagcggaagt
    15601 cgtgtgatta gtatctgcta cttcgaacaa gagaacgact ggtgggtttg taaacacatt
    15661 aaaaaaccaa tcagatccac ggtcctttca ctggattggc atcctaataa cgtactcctg
    15721 gcggcgggtt cctgtgattt taagtgccgc atcttctccg cctatattaa ggaggtggaa
    15781 gaaagaccag ccccaactcc atggggcagt aagatgcctt tcggcgaact catgttcgaa
    15841 tctagttctt cttgcggatg ggttcatggc gtttgttttt ctgcaagcgg tagtcgcgtg
    15901 gcttgggtta gccacgacag cacggtttgc ctggcagacg cagataaaaa gatggctgta
    15961 gcaacccttg cgagcgagac tttgccactc ttggcactca ccttcatcac tgacaactcg
    16021 ctcgttgcgg caggccatga ttgctttccg gtactgttta cttacgacgc cgcagctggt
    16081 atgttgtctt tcggcggtcg ccttgacgtc cccaagcaga gctctcagcg cggtctcact
    16141 gcccgtgaaa gattccaaaa tcttgataag aaggcatcct ccgaaggagg tacagccgcg
    16201 ggcgctggat tggacagtct gcacaaaaac tcggtgtccc aaatatcggt attgtccgga
    16261 ggaaaagcta aatgcagtca gttctgcaca acgggaatgg atggaggtat gagcatttgg
    16321 gacgtcaaaa gcctcgaatc agccctcaaa gatttgaaaa ttaaataaac tagtggtacc
    16381 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
    16441 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    16501 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    16561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    16621 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa
    16681 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc
    16741 atttaaataa atctatatct caatcggggt tacgtcgata ccccaccgag accccattgg
    16801 gaccaatacg cccgcgtttc ttccttttcc ccaccccaac ccccaagttc gggtgaaggc
    16861 ccagggctcg cagccaacgt cggggcggca agccctgcca tagccactac gggttaatta
    16921 aaaacggttc acatagctta gtttcaatca ggagatccga accagataag tgaaatctag
    16981 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc
    17041 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag
    17101 ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca
    17161 aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctacttt
//
