LOCUS       sequence-341834-        2005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   White MA, Lin W, Cheng X
  TITLE     Discovery of COVID-19 Inhibitors Targeting the SARS-CoV-2 Nsp13 
            Helicase.
  JOURNAL   J Phys Chem Lett. 2020 Nov 5;11(21):9144-9151. doi: 
            10.1021/acs.jpclett.0c02421. Epub 2020 Oct 14.
  PUBMED    33052685
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.0c02421"; journalName: "J Phys Chem Lett"; 
            date: "2020-11-5- 5"; volume: "11"; issue: "21"; pages: "9144-9151"
COMMENT     Sequence Label: pET28a-SARS-CoV-2 NSP13
FEATURES             Location/Qualifiers
     source          1..2005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1960..1977
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat gactggtgga
      121 cagcaaatgg gtcgcggatc cgccatggct gtaggtgctt gtgtattgtg caattcacag
      181 acttcacttc gttgcggtgc ctgtattagg agaccattcc tatgttgcaa gtgctgctat
      241 gaccatgtca tttcaacatc acacaaatta gtgttgtctg ttaatcccta tgtttgcaat
      301 gccccgggtt gtgatgtcac tgatgtgaca caactgtatc taggaggtat gagctattat
      361 tgcaagtcac ataagcctcc cattagtttt ccattatgtg ctaatggtca ggtttttggt
      421 ttatacaaaa acacatgtgt aggcagtgac aatgtcactg acttcaatgc gatagcaaca
      481 tgtgattgga ctaatgctgg cgattacata cttgccaaca cttgtactga gagactcaag
      541 cttttcgcag cagaaacgct caaagccact gaggaaacat ttaagctgtc atatggtatt
      601 gccactgtac gcgaagtact ctctgacaga gaattgcatc tttcatggga ggttggaaaa
      661 cctagaccac cattgaacag aaactatgtc tttactggtt accgtgtaac taaaaatagt
      721 aaagtacaga ttggagagta cacctttgaa aaaggtgact atggtgatgc tgttgtgtac
      781 agaggtacta cgacatacaa gttgaatgtt ggtgattact ttgtgttgac atctcacact
      841 gtaatgccac ttagtgcacc tactctagtg ccacaagagc actatgtgag aattactggc
      901 ttgtacccaa cactcaacat ctcagatgag ttttctagca atgttgcaaa ttatcaaaag
      961 gtcggcatgc aaaagtactc tacactccaa ggaccacctg gtactggtaa gagtcatttt
     1021 gccatcggac ttgctctcta ttacccatct gctcgcatag tgtatacggc atgctctcat
     1081 gcagctgttg atgccctatg tgaaaaggca ttaaaatatt tgcccataga taaatgtagt
     1141 agaatcatac ctgcgcgtgc gcgcgtagag tgttttgata aattcaaagt gaattcaaca
     1201 ctagaacagt atgttttctg cactgtaaat gcattgccag aaacaactgc tgacattgta
     1261 gtctttgatg aaatctctat ggctactaat tatgacttga gtgttgtcaa tgctagactt
     1321 cgtgcaaaac actacgtcta tattggcgat cctgctcaat taccagcccc ccgcacattg
     1381 ctgactaaag gcacactaga accagaatat tttaattcag tgtgcagact tatgaaaaca
     1441 ataggtccag acatgttcct tggaacttgt cgccgttgtc ctgctgaaat tgttgacact
     1501 gtgagtgctt tagtttatga caataagcta aaagcacaca aggataagtc agctcaatgc
     1561 ttcaaaatgt tctacaaagg tgttattaca catgatgttt catctgcaat caacagacct
     1621 caaataggcg ttgtaagaga atttcttaca cgcaatcctg cttggagaaa agctgttttt
     1681 atctcacctt ataattcaca gaacgccgta gcttcaaaaa tcttaggatt gcctacgcag
     1741 actgttgatt catcacaggg ttctgaatat gactatgtca tattcacaca aactactgaa
     1801 acagcacact cttgtaatgt caaccgcttc aatgtggcta tcacaagggc aaaagttggc
     1861 attttgtgca taatgtctga tagagatctt tatgacaaac tgcaatttac aagtctagaa
     1921 ataccacgtc gcaatgtggc tacattacaa tgactcgagc accaccacca ccaccactga
     1981 gatccggctg ctaacaaagc ccgaa
//
