LOCUS       sequence-341889-        6653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of sarA with its STAT3-binding YxxQ motif 
            mutated to FxxQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, 
            Brennan RG, Ko DC
  TITLE     The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor 
            Signaling to Activate STAT3.
  JOURNAL   Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 
            10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.
  PUBMED    31901521
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.11.012"; journalName: "Cell Host Microbe"; 
            date: "2020-01-8- 8"; volume: "27"; issue: "1"; pages: "129-139.e4"
COMMENT     Sequence Label: pWSK129-stm2585_Y167F
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(754..773)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             765..1580
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             765..1580
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(838..857)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     protein_bind    1678..1699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1714..1719,1720..1737,1738..1744)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1752..1768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1757..1779
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1776..1792
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1776..1792
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1811..1831
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1813..1831
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3246..3265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3247..3265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3275..3292)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3275..3291)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3284..3306)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3433..3861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3520..3539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3730..3751
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4861..5083
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5131..6081
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 agatccatta tgttaaacgg gcgagtttac atctcaaaac cgcccgctta acaccatcag
       61 aaatcctcag cgcgatttta agcaccaacc cccccccgta acacccaaat ccatactgaa
      121 agtggctttg ttgaataaat cagatttcgg gtaagtctcc cccgtagcgg gttgtgtttt
      181 caggcaatac gcacgctttc aggcatacct gctttcgtca ttttgttcag cgctcgtacc
      241 agggccatag cctccgcaac ctgaccatcg tagtcacgca gcgtcagtga acccccgaac
      301 agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa tcaataaagc
      361 cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca gtcgtgatgg
      421 caaggtcaga atagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg
      481 cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt
      541 tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt
      601 cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc
      661 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
      721 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
      781 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      841 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
      901 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      961 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     1021 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
     1081 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     1141 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     1201 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     1261 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     1321 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     1381 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1441 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1501 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     1561 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     1621 cgctgactgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     1681 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1741 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     1801 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
     1861 cggccgctct agactacatg ccaactttac ttagtatcat attttcttgc tacatatctg
     1921 tttggcaagg gtatgaataa aaaacaagat taatttaatt gatatcgcaa cagataagca
     1981 cctgcattta tctttaaact tttttatggc caaccagatt tttcaccatc tgcgcacgtt
     2041 tattcgggca tgcgttgaaa acctgcaaaa aaagaaaaat atcgatgctt attatttttt
     2101 ctttaattaa attttcgctc aacaaactta attattaatc caatgacagt gaagtgtgaa
     2161 ctatgctgag cataaacgac atcaatagca agggcaaggg caagggcaag ggcaagggca
     2221 agggcaaggg caagggcaag ggcaatctga gtatttacag gtgaaattat gaaaaatagt
     2281 atatttttac cattgcttct ggtgctgtcg gcaacgacct tttcggcatt ggtgatggct
     2341 gccagtcatt ctccccacca gataatacaa aacatttttc cagtggctgg ccgccagata
     2401 tcctgaagca actgaattgg tctcaggttt gtggttgtta gtatactgaa tattacttca
     2461 ggatggcatg ttttattgac accagcctga tttttcacaa cgttatatta cccgtttaca
     2521 gataattccc agctaataaa tcaggaagtt ccagatgtaa cagaagttat gaggtgtatt
     2581 aattgatagg tctaacaggc ttgaattact tttctttaaa aactactgca tgtaaaaggg
     2641 tctcctcttg ttgtgatgag attcgtatat atttatatct tagtgattta tggatcatac
     2701 ttgtggtttt ccttaggagg taacatgttt acaattaata gtactaacag ggtggcgagc
     2761 acaatcgctc catatgcatg tgtaagcgat gttaatttag aggacaaggc tacattttta
     2821 gatgaacata cctcaataca tgcgaatgat tcttctttgc aatgctttgt cttaaatgat
     2881 cagcatgttc cgcagaatac attagctaca gatgttgaag gctacaatag aggattacag
     2941 gaacgtataa gcctcgagta ccagcccctg gaaagcattg tttttctact cggcacccct
     3001 gcagttttag agactaaaga gtctttatca ttaccagttt ccccggatgc tttaacccaa
     3061 aaactattaa gcattagtag caatgatgaa tgtaaattat caggtagcac cagttgcact
     3121 accccagcca gtcataaccc accgtccggt tttatcgctc aatacaggca ttctgcagag
     3181 gttttcccgg atgaataaaa caaagcgtta taaatagctg cgccgagggc ccggtaccca
     3241 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3301 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3361 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3421 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3481 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3541 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3601 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3661 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3721 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3781 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3841 aacaaaaatt taacgcgaat tttaacaaaa ttcgaccgat gcccttgaga gccttcaacc
     3901 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     3961 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     4021 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     4081 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     4141 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     4201 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     4261 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     4321 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     4381 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     4441 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     4501 aagccggcgg cacctcgcta acggattcac cactccgcag acccgccata aaacgccctg
     4561 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
     4621 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca gcgaactgaa
     4681 tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
     4741 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     4801 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     4861 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     4921 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     4981 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     5041 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
     5101 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     5161 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     5221 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     5281 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     5341 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     5401 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
     5461 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     5521 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     5581 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     5641 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     5701 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
     5761 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     5821 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     5881 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     5941 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     6001 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     6061 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     6121 actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat
     6181 atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac gagtttttgg
     6241 tgcattcaaa gctgttcacc atgaacagat cgacaatgta acagatgaac agcatgtaac
     6301 acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa ttgaacacct
     6361 gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg cgatgctgct
     6421 tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg ggaaaaaatc
     6481 atggcaattc tggaagaaat agcgctttca gccggcaaac cggctgaagc cggatctgcg
     6541 attctgataa caaactagca acaccagaac agcccgtttg cgggcagcaa aacccgtact
     6601 tttggacgtt ccggcggttt tttgtggcga gtggtgttcg ggcggtgcgc gca
//
