LOCUS       sequence-341891-        8157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of sarA-gp130 chimera under native promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, 
            Brennan RG, Ko DC
  TITLE     The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor 
            Signaling to Activate STAT3.
  JOURNAL   Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 
            10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.
  PUBMED    31901521
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.11.012"; journalName: "Cell Host Microbe"; 
            date: "2020-01-8- 8"; volume: "27"; issue: "1"; pages: "129-139.e4"
COMMENT     Sequence Label: pWSK129-stm2585-gp130
FEATURES             Location/Qualifiers
     source          1..8157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(789..808)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             800..1615
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             800..1615
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(873..892)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3025..3042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3159..3180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3195..3200,3201..3218,3219..3225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3233..3249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3238..3260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3257..3273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3257..3273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3292..3312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3294..3312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3349..3365
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3349..3365
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4744..4760)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4745..4761)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4785..4804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4786..4804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4814..4831)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4814..4830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4823..4845)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4972..5400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5059..5078)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5269..5290
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      6400..6622
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6670..7620
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tttttgtggc gagtggtgtt cgggcggtgc gcgcaagatc cattatgtta aacgggcgag
       61 tttacatctc aaaaccgccc gcttaacacc atcagaaatc ctcagcgcga ttttaagcac
      121 caaccccccc ccgtaacacc caaatccata ctgaaagtgg ctttgttgaa taaatcagat
      181 ttcgggtaag tctcccccgt agcgggttgt gttttcaggc aatacgcacg ctttcaggca
      241 tacctgcttt cgtcattttg ttcagcgctc gtaccagggc catagcctcc gcaacctgac
      301 catcgtagtc acgcagcgtc agtgaacccc cgaacagctg taatccgggc agcgcaacgg
      361 aacattcatc agtgtaaaaa tggaatcaat aaagccctgc gcagcgcgca gggtcagcct
      421 gaatacgcgt ttaatgacca gcacagtcgt gatggcaagg tcagaatagc gctgaggtct
      481 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca
      541 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
      601 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
      661 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
      721 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      781 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      841 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      901 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
      961 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1021 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1081 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc
     1141 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1201 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     1261 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1321 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
     1381 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1441 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1501 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1561 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1621 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt
     1681 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     1741 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     1801 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg
     1861 tatgagtcag caacaccttc ttcacgaggc agacctcagc gctattctga ccttgccatc
     1921 acgactgtgc tggtcattaa acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt
     1981 attgattcca tttttacact gatgaatgtt ccgttgcgct gcccggatta cctgcatatt
     2041 aaagagctgt atgtttctca gtgcgatcga aataagggca cgggtaaagc gataatgcgc
     2101 tttatagccc ggcttgcgct tgaacaggaa tgccttagcc ttagctggaa cgctgaaaaa
     2161 tccaaccccg gcgctaaccg tttttatcag gctctgggag gcagaataaa tgatcatatc
     2221 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
     2281 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     2341 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     2401 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     2461 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     2521 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     2581 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2641 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2701 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2761 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2821 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2881 catatcacca gctcaccgtc tttcattgcc atacggaatt gatctcacat gttctttcct
     2941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3001 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3061 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3121 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     3301 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
     3361 ggatccctct agactacatg ccaactttac ttagtatcat attttcttgc tacatatctg
     3421 tttggcaagg gtatgaataa aaaacaagat taatttaatt gatatcgcaa cagataagca
     3481 cctgcattta tctttaaact tttttatggc caaccagatt tttcaccatc tgcgcacgtt
     3541 tattcgggca tgcgttgaaa acctgcaaaa aaagaaaaat atcgatgctt attatttttt
     3601 ctttaattaa attttcgctc aacaaactta attattaatc caatgacagt gaagtgtgaa
     3661 ctatgctgag cataaacgac atcaatagca agggcaaggg caagggcaag ggcaagggca
     3721 agggcaaggg caagggcaag ggcaatctga gtatttacag gtgaaattat gaaaaatagt
     3781 atatttttac cattgcttct ggtgctgtcg gcaacgacct tttcggcatt ggtgatggct
     3841 gccagtcatt ctccccacca gataatacaa aacatttttc cagtggctgg ccgccagata
     3901 tcctgaagca actgaattgg tctcaggttt gtggttgtta gtatactgaa tattacttca
     3961 ggatggcatg ttttattgac accagcctga tttttcacaa cgttatatta cccgtttaca
     4021 gataattccc agctaataaa tcaggaagtt ccagatgtaa cagaagttat gaggtgtatt
     4081 aattgatagg tctaacaggc ttgaattact tttctttaaa aactactgca tgtaaaaggg
     4141 tctcctcttg ttgtgatgag attcgtatat atttatatct tagtgattta tggatcatac
     4201 ttgtggtttt ccttaggagg taacatgttt acaattaata gtactaacag ggtggcgagc
     4261 acaatcgctc catatgcatg tgtaagcgat gttaatttag aggacaaggc tacattttta
     4321 gatgaacata cctcaataca tgcgaatgat tcttctttgc aatgctttgt cttaaatgat
     4381 cagcatgttc cgcagaatac attagctaca gatgttgaag gctacaatag aggattacag
     4441 gaacgtataa gcctcgagta ccagcccctg gaaagcattg tttttctact cggcacccct
     4501 gcagttttag agactaaaga gtctttatca ttaccagttt ccccggatgc tttaacccaa
     4561 aaactattaa gcatcagcag cagcgacgag aacgaaagca gccagaacac cagcagcacc
     4621 gtgcaataca gcaccgtggt tcacagcggt tatcgtcacc aggttccgag cgtgcaagtt
     4681 ttcccggatg aataaaacaa agcgttataa atagctgcgc cgagaattcg atatcaagct
     4741 tatcgatacc gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     4801 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4861 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4921 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     4981 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5041 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5101 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5161 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5221 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5281 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5341 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5401 ttaacaaaat tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     5461 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     5521 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     5581 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     5641 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     5701 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     5761 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     5821 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     5881 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     5941 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     6001 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     6061 cggattcacc actccgcaga cccgccataa aacgccctga gaagcccgtg acgggctttt
     6121 cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac
     6181 ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac
     6241 tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg
     6301 gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc
     6361 gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga
     6421 tctattcttt ttatcttttt ttattctttc tttattctat aaattataac cacttgaata
     6481 taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca
     6541 agttttccag caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact
     6601 agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa
     6661 ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat
     6721 gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg
     6781 attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag
     6841 atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg
     6901 atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg
     6961 acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg
     7021 ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt
     7081 gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa
     7141 actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa
     7201 aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat
     7261 ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg
     7321 ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac
     7381 cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc ctacctacat
     7441 aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt
     7501 gaggcaaaat ttttgagtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg
     7561 ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga
     7621 ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac
     7681 aactgttcac cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt
     7741 aaaaaagata gatacatcag agcttttacg agtttttggt gcattcaaag ctgttcacca
     7801 tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac
     7861 cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca
     7921 acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac
     7981 aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata
     8041 gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga ttctgataac aaactagcaa
     8101 caccagaaca gcccgtttgc gggcagcaaa acccgtactt ttggacgttc cggcggt
//
