LOCUS       sequence-34194-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1320-HQ858822
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(557..656)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(673..692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(674..692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(697..713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(697..713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccccctt caccatggcc
      121 gggattggtc cgatcaggca ggactgggag ccggtggtgg tgcggaagaa ggcgcccacc
      181 gccgccgcca agaaggatga gaaggccgtc aacgccgccc gccgctccgg cgccgagatc
      241 gagaccatga agaagtataa cgctggaaca aacaaggcgg cgtccagtgg cacatccctc
      301 aacaccaagc ggctggatga cgacaccgag agccttgccc atgagcgtgt ctcaagtgac
      361 ctgaagaaaa acctcatgca agcaaggctg gacaagaaga tgacccaggc acagcttgca
      421 cagatgatca atgagaagcc ccaggtgatc caggagtacg agtcaggtaa agctattccg
      481 aaccagcaga tcatcgggaa gcttgaaagg gctcttggaa caaagctgcg cggcaagaaa
      541 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
      601 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      661 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      721 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      781 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgtt
//
