LOCUS       sequence-341943-        8745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination transgenesis vector for expressing ReaChR fused with 
            citrine in zebrafish spinal motor neurons using 3 tandem repeats of 
            the mnx1 enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Harris JM, Wang AY, Boulanger-Weill J, Santoriello C, Foianini S, 
            Lichtman JW, Zon LI, Arlotta P
  TITLE     Long-Range Optogenetic Control of Axon Guidance Overcomes 
            Developmental Boundaries and Defects.
  JOURNAL   Dev Cell. 2020 Jun 8;53(5):577-588.e7. doi: 
            10.1016/j.devcel.2020.05.009.
  PUBMED    32516597
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2020.05"; journalName: "Dev Cell"; date: 
            "2020-06-8- 8"; volume: "53"; issue: "5"; pages: "577-588.e7"
COMMENT     Sequence Label: pDestTol2CG2-mnx1:ReaChR-citrine
FEATURES             Location/Qualifiers
     source          1..8745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1665..1689
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2894..2896,2897..2899,2900..3610)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(3614..3638)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3640..3774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3837..3857)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3865..3881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4151..4285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4333..5046,5047..5049,5050..5052))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4336..5046,5047..5049,5050..5052))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(6275..6462,6463..6474)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6508..6524)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6508..6524)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6561..6581)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6561..6579)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6600..6616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6613..6635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6624..6640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6648..6654,6655..6672,6673..6678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6693..6714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6831..6848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7002..7590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7082..7101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7761..8552,8553..8621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8384..8403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8622..8726)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgccctttc ctcgagagtg gttagctgat
     1261 gaattgagga aaactaatcc gctttattgg tagacaggtt aagggcaaca gagtgtcaat
     1321 aattctctcc ctgacctctt tctccattag cttaaagagg cttataagag ggcatgccga
     1381 gagtggttag ctgatgaatt gaggaaaact aatccgcttt attggtagac aggttaaggg
     1441 caacagagtg tcaataattc tctccctgac ctctttctcc attagcttaa agaggcttat
     1501 aagaggccta ggcgagagtg gttagctgat gaattgagga aaactaatcc gctttattgg
     1561 tagacaggtt aagggcaaca gagtgtcaat aattctctcc ctgacctctt tctccattag
     1621 cttaaagagg cttataagag gaagcttgta agggcgaatt cgacccaagt ttgtacaaaa
     1681 aagcaggctc cgcggccaat ctgctcagag aggacagagt gggcaggagc cagcattggg
     1741 tatataaagc tgagcagggt cagttgcttc ttacgtttgc ttctgagtct gttgtgttga
     1801 cttgcaacct cagaaacaga catccgcccc cttcaccatg gtgagcagaa gaccctggct
     1861 gctggccctg gccctggccg tggccctggc cgccggcagc gccggcgcca gcaccggcag
     1921 cgacgccacc gtgcccgtgg ccacccagga cggccccgac tacgtgttcc acagagccca
     1981 cgagagaatg ctgttccaga ccagctacac cctggagaac aacggcagcg tgatctgcat
     2041 ccccaacaac ggccagtgct tctgcctggc ctggctgaag agtaacggca ccaacgccga
     2101 gaagctggcc gccaacatcc tgcagtgggt ggtgtttgcg ctgagcgtgg cgtgcctggg
     2161 ctggtatgcg tatcaggcgt ggcgcgcgac ctgcggctgg gaagaagtgt atgtggcgct
     2221 gattgaaatg atgaaaagca ttattgaagc gtttcatgaa tttgatagcc cggcgaccct
     2281 gtggctgagc agcggcaacg gcgtggtgtg gatgcgctat ggcgaatggc tgctgacctg
     2341 ccccgtgatt ctgattcatc tgagcaacct gaccggcctg aaagatgatt atagcaaacg
     2401 caccatgggc ctgctggtga gcgacgtggg ctgcattgtg tggggcgcga ccagcgcgat
     2461 gtgcaccggc tggaccaaaa ttctgttttt tctgattagc ctgagctatg gcatgtatac
     2521 ctattttcat gcggccaaag tgtatattga agcgtttcat accgtgccga aaggcctgtg
     2581 cagacagctg gtgagagcca tggcctggct gttcttcgtg agctggggca tgttccccgt
     2641 gctgttcctg ctgggccccg agggcttcgg ccatattagc ccgtatggca gcgcgattgg
     2701 ccatagcatt ctggatctga ttgcgaagaa catgtggggc gtgctgggca actatctgcg
     2761 cgtgaaaatt catgaacata ttctgctgta tggcgatatt cgcaaaaaac agaaaattac
     2821 cattgcgggc caggaaatgg aagtggaaac cctggtggcg gaagaagaag ataagtacga
     2881 gagcagcctc gagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2941 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3001 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3061 gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc
     3121 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3181 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3241 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3301 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3361 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3421 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3481 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     3541 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3601 gctgtacaag ccgacccagc tttcttgtac aaagtggggg atccagacat gataagatac
     3661 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3721 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3781 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttccaac
     3841 tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc
     3901 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3961 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4021 ataaacaaga gatctgatct agaggatcat aatcagccat accacatttg tagaggtttt
     4081 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4141 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4261 caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc tagaggctcg
     4321 agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     4381 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     4441 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     4501 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     4561 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     4621 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     4681 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     4741 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     4801 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     4861 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     4921 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     4981 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     5041 cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg tcactggctt
     5101 actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa aggtctgcag
     5161 tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga gccctgataa
     5221 ctgcacaagt gctcagattc cagcagggtg gaaaatgaga taaagtgtgc agatggggag
     5281 ggggacgtga atgagagatt tgagggatga aaaggatgga tgaacgcatt gaaaatagcc
     5341 cctttcacaa aataatacca gtaaattgtc atataattaa tgaccagacc tttacctgta
     5401 aatgatgtga tgttcatata cagaagaatg ccaacaattt acagatgatt ttacaaattc
     5461 ttaattttcc ttttctaaat ggatttgttc acacattatc tcttcaaagc aattgaattt
     5521 tctggaaaag attggctgtg tgtgtaaaag gtgctactga ggaatttaat gtgacatgga
     5581 atgaagcaaa cagcagccta agacaggatg gcaggaaaaa tgtcacatgt ttaaaataag
     5641 agtgaaacca acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag ctcttgtaca
     5701 aacagtgctt gtgactgtga aattcggggt ttgcctggat tgtgttaaat gtgtgcttgg
     5761 atgtatcata aaaaagaatc ggttctctgt atttgacatt ttctgtaatt tatctaaagt
     5821 tacaactggc tatgcctgat ttttatcact attgaatcaa tcattttaaa gaatgttaag
     5881 tctgctgatt tggagagctc atttacattt attttgaatg tctcttattt aacacaactg
     5941 atttaagctt tagatctgcg aagatacggc cacgggtgct cttgatcctg tggctgattt
     6001 tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt cttccactga
     6061 ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg gtaatagatg
     6121 tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca tataatgtga
     6181 aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc agcgcaattc
     6241 aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa ataagtactt
     6301 tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa aaaatgtaat
     6361 taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa atccccaaaa
     6421 ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc tctggttctt
     6481 gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc gccaccgcgg
     6541 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     6601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7621 gacgctcagt ggaacgaaaa ctcacgttaa ggcattttgg tcatgagatt atcaaaaagg
     7681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtg
//
