LOCUS       sequence-341950-        8108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger 
            operators upstream of Cyc1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
  TITLE     Mapping the dynamic transfer functions of eukaryotic gene 
            regulation.
  JOURNAL   Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003.
  PUBMED    34469745
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003."
COMMENT     Sequence Label: pJL29
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        262..669
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     3572..3591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3808..3830
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3823..3839
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3849..3868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3849..3867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3892..3908
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3893..3909
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5012..5201
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5240..5260)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5240..5258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5279..5295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5279..5295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5292..5314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5303..5319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5327..5333,5334..5351,5352..5357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5510..5527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5681..6269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5761..5780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6440..7231,7232..7300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7063..7082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7301..7405)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgcaggga tcctaatctg gccgaggaga acttctagta tatctacata cctaatatta
       61 ttgccttatt aaaaatggaa tcccaacaat tacatcaaaa tccacattct cttcaaaatc
      121 aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat aggataatta
      181 tactctattt ctcaacaagt aattggttgt ttggccgagc ggtctaaggc gcctgattca
      241 agaaatatct tgaccgcagt taactgtggg aatactcagg tatcgtaaga tgcaagagtt
      301 cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta
      361 tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg
      421 catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg
      481 gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag
      541 ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac
      601 tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt tcaaggatat
      661 accattctaa tgtctgcccc taagttacca atagatagcg gtttaatata aggtttcaac
      721 aagaaatatt ttgtgaaaat caaaagttaa tagtttcact gtcgaaaata tgaaagttta
      781 agttttcagt tcatcccatt gtagttatca aatttctgtg tttcccgtat atgtgtgctc
      841 tgtattcatc gctatcttga aactggttat gatcgttgcg catgtgacct gtatcgctca
      901 aaagagcccg gcaccggtac cgggcaaaag cccctagcaa gctaaaatga ttcaactttc
      961 gtagtaattt gttcaaccgg tcacagtaaa gtcaatgact aacttttgaa tcgaggatgt
     1021 atttccaatt gaaagcaatg tatatataaa ccattgtgtt gaagcttgtc ttacagtttt
     1081 cttgtatcct cccattgttc atatagtact taaagtacca taatccttaa agaacaaact
     1141 tgaaagagaa tacactatac acaactaata aataaagaat aatcatggct gtgaccaaga
     1201 caattgtagt tctaccaggt gaccatgttg gtcaagaaat cactgaagag gccattaaag
     1261 ttttaaatgc tattcaggaa tgtcgtccag acaaggtcaa tttcaagttt gatcatcatt
     1321 tgatcggtgg tgctgcaatt gatgccactg gtgttccatt accagacgaa gctttggagg
     1381 cctctaagaa agctgatgcc gtgcttctgg gtgctgttgg tggtccaaaa tggggtactg
     1441 gcgctgtcag accagaacaa ggtcttttga aaatccgtaa ggagttgcaa ttgtatgcta
     1501 atctaagacc atgtaatttt gcatctgatt ccttactaga tctatcgcca ttgaagcctg
     1561 aaattgcaag aggtacagat ttcgtcgttg ttagagaact agtgggtggt atttatttcg
     1621 gggagagaaa agaagatgaa ggtgatggtg tcgcctggga tagcgaaaag tattctgtgc
     1681 ccgaagttca aagaatcact agaatggctg ctttcatggc tctgcaacac aacccacctt
     1741 tacctatttg gtctctagat aaagccaacg ttctagcatc atcaagatta tggagaaaaa
     1801 cagtggaaga gacgattaag aatgaattcc cacaattgac tgttcaacat caattgattg
     1861 attccgctgc tatgatcttg gttaagaacc caactcatct aaatggtatt attatcacaa
     1921 acaatatgtt tggtgatatt atctccgatg aagcctccgt gatcccaggt tcgttaggat
     1981 tacttccatc tgcctccttg gcatctcttc cagataagaa tacggcattt ggtttatatg
     2041 agccatgtca tggctctgct ccagacttac caaagggaaa ggttaacccc gtagcaacaa
     2101 tcctatctgc tgctatgatg ttgaagttgt ctctggacct gttcgaagaa ggtgaaatta
     2161 tagaacaagc agtcaagaaa gtgttggatt ctggtatcag aacagcggat ctaaagggta
     2221 ccaactctac tactgaggtc ggtgatgcag tagcgaaagc tgtcagggaa ctattagctt
     2281 agacttagta gatgtacagt tgcatacata agtctgaaca gaaagaagta aatatcatat
     2341 ctagttcggt ttagaaatca gtgactatat tggttttgac cagatcgcaa tccttgcaag
     2401 catttagtaa tgactatcaa accggtgaat catctgataa ggctatatta gcttgtgcat
     2461 tcgcatgtat cgggaaacga acggtaccgg gcccgcagat ccgccaggcg tgtatatata
     2521 gcgtggatgg ccaggcaact ttagtgctga cacatacagg catatatata tgtgtgcgac
     2581 gacacatgat catatggcat gcatgtgctc tgtatgtata taaaactctt gttttcttct
     2641 tttctctaaa tattctttcc ttatacatta ggacctttgc agcataaatt actatacttc
     2701 tatagacaca caaacacaaa tacacacact aaattaataa tgactgaact cgagggagca
     2761 aggccttgac ccatccttac cgtaccgatc cgatccgtac cgctccgagc catcccgttc
     2821 cgcaccgcac cgttccgaac cgctccgtcc cgctccgtaa tagtaaccta aggcgaccga
     2881 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
     2941 aatcagggac gagaatttgt ctgccgaccg ggtgatattt tgctgttccc gccaggagag
     3001 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
     3061 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggt
     3121 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
     3181 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
     3241 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
     3301 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
     3361 agcgtcgcag atctcatcag ccctagggac tgctccacgg gtgtcgctcc ttcatctgac
     3421 aatatgcagc cgctaccacc aaggattcat acaacgaacg gtgatgttgt catagattcg
     3481 gcacatttcc cttgtaggtg tgaaatggct caaagacgac agaccgctcg ccggtaacag
     3541 taaccccgcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3601 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3661 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     3721 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3781 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3841 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
     3901 acggtatcga taagcttgat atcgaattca gagtgaggac tcgaaaatat taatagagtg
     3961 aggacggatc cattatggga gaactagtca gatccgccag gcgtgtatat atagcgtgga
     4021 tggccaggca actttagtgc tgacacatac aggcatatat atatgtgtgc gacgacacat
     4081 gatcatatgg catgcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct
     4141 aaatattctt tccttataca ttaggacctt tgcagcataa attactatac ttctatagac
     4201 acacaaacac aaatacacac actaatctag atattaaaat ggcaactagc ggcatggtta
     4261 gtaaaggaga agaaaataac atggcaatca ttaaggagtt catgagattc aaagttcaca
     4321 tggaaggttc tgtaaatgga catgaatttg aaatagaagg tgaaggagaa ggaaggcctt
     4381 atgaaggaac ccaaaccgcg aagctaaaag ttactaaggg tggcccatta ccatttgcat
     4441 gggatatcct tagccctcaa ttcatgtatg ggtcaaaggc ttatgtcaag caccccgccg
     4501 acattccaga ctatctaaag ttatcttttc ccgaagggtt taagtgggag cgtgtgatga
     4561 acttcgaaga cggtggcgtg gtaacagtga ctcaggattc gtccctgcaa gatggtgaat
     4621 ttatctacaa agtcaaatta agaggaacta actttccatc tgacggcccg gttatgcaaa
     4681 aaaagacaat gggctgggag gcctcctcag aacgaatgta ccctgaagat ggtgccttga
     4741 agggtgagat taaacaaaga ttgaaattga aagatggtgg acattatgac gctgaggtta
     4801 aaacgacata caaagctaag aaacctgtcc agctcccagg tgcttacaat gtaaatataa
     4861 aacttgatat tacatcacat aatgaagatt atacgatagt tgaacaatac gaaagggctg
     4921 aggggagaca tagtactggt ggcatggatg aactatacaa aggttctggt accgcataac
     4981 tcgagtaagc ttggtaccgc ggctagctaa gatccgctct aaccgaaaag gaaggagtta
     5041 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
     5101 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
     5161 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa gatccggccg ccaccgcggt
     5221 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     5281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac ataggagccg
     5341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt
     5401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6121 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtccact
     7441 tagccgatcc gaaccggacc gctccgagcc gtcccgtacc gcaccgcgcc gttccgagcc
     7501 ggacctcccg ctccgtaccg ttcaccagag gccattcagg ccgttgatac ccgggactgc
     7561 tcggttacca tattcgcgta cgccaccgaa gtcggtgatg ctgtcgccga agaagttaag
     7621 aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat
     7681 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga
     7741 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa
     7801 tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta
     7861 acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac agaaaatcat
     7921 gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa aaaatacaac
     7981 aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata ccttcttgaa
     8041 ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa acggccttaa
     8101 cgacgtag
//
