LOCUS       sequence-341957-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment 
            of TF. Used with pJL32..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
  TITLE     Mapping the dynamic transfer functions of eukaryotic gene 
            regulation.
  JOURNAL   Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003.
  PUBMED    34469745
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003."
COMMENT     Sequence Label: pJL30
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        245..459
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        905..1346
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     protein_bind    905..1022
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    1230..1248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1317..1340
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1373..1438
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1445..1465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1829..3664
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="KMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQF
                     YPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYD
                     PVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIE
                     SVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNE
                     FIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEE
                     SADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLV
                     DAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYI
                     SGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKAS
                     GVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEP
                     GLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVF
                     FVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     terminator      5344..5541
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5963..5982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5964..5982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5989..6006)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5989..6005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5998..6020)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6146..6601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6233..6252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6443..6464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6957..6976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7076..7098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7136..7154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7239..7343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7344..7412,7413..8204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7562..7581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8375..8963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8864..8883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9117..9134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9251..9272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9287..9292,9293..9310,9311..9317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9325..9341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9330..9352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9349..9365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9349..9365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9386..9404
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacctactg cgccaattga tgacaataca gacgatgata
       61 acaaaccgaa gttatctgat gtagaaaagg attaaagatg ctaagagata gtgatgatat
      121 ttcataaata atgtaattct atatatgtta attacctttt ttgcgaggca tatttatggt
      181 gaaggataag ttttgaccat caaagaaggt taatgtggct gtggtttcag ggtccataaa
      241 gcttttcaat tcatcttttt tttttgttct tttttttgat tccggtttct ttgaaatttt
      301 tttgattcgg taatctccga gcagaaggaa gaacgaagga aggagcacag acttagattg
      361 gtatatatac gcatatgtgg tgttgaagaa acatgagatt gcccagtatt cttaacccaa
      421 ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag
      481 gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa
      541 aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta
      601 gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
      661 ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta
      721 ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg
      781 ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca
      841 aatacctcga gggagcaagg caggtggtcg acgcgaattc ggatccacta gtggtaccga
      901 agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg
      961 cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc
     1021 cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt
     1081 aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg
     1141 cgattagttt tttagcctta tttctggggt actgcagcag cgaagcgatg atttttgatc
     1201 tattaacaga tatataaatg caaaaactgt ccctatcagt gatagagata tccctatcag
     1261 tgatagagat attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata
     1321 tacctctata ctttaacgtc aaggagaaaa aaccctgggc cctaaaaaaa tggactacaa
     1381 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat
     1441 ggcccccaag aaaaagagga aggtgggcat tcacggggtg ccgggtggag gttctggttc
     1501 tagacccggg gagcgcccct tccagtgtcg catttgcatg cggaactttt cgcgccagga
     1561 caggcttgac aggcataccc gtactcatac cggtgaaaaa ccgtttcagt gtcggatctg
     1621 tatgcgaaat ttctcccaga aggagcactt ggcggggcat ctacgtacgc acaccggcga
     1681 gaagccattc caatgccgaa tatgcatgcg caacttcagt cgccgcgaca acctgaaccg
     1741 gcacctaaaa acccacctga gaggatccgg aggttctggc ggtggaagtg gtggcggagg
     1801 tagcggtggt ggcagcggtt cctgcaggaa gatggacaaa aagactatag tttggtttag
     1861 aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg aaggatctgt
     1921 ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg gaagagcttc
     1981 aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg ctcttggatc
     2041 tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt gtatccgcgt
     2101 taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt tagttcggga
     2161 ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct acaatggaga
     2221 tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta cgagtttcaa
     2281 ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc ctcctccttg
     2341 gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga ttgaagaact
     2401 agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag cttggtctcc
     2461 aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt tgatagatta
     2521 tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc cgtatctcca
     2581 tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac aaattatatg
     2641 ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta ggggaatcgg
     2701 tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg agcaatcgtt
     2761 gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca aggcctggag
     2821 acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt gggctaccgg
     2881 atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt ttcttctcct
     2941 tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg atttggaatg
     3001 tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg agcttgatcg
     3061 cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt acataaggca
     3121 atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat gggacgctcc
     3181 tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga aacccattgt
     3241 agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc gtgaagcaca
     3301 gatcatgatc ggagcagcac ctgatgagat tgtagcagat agcttcgagg ccttaggggc
     3361 taataccatt aaagaacctg gtctttgccc atctgtgtct tctaatgacc aacaagtacc
     3421 ttcggctgtt cgttacaacg ggtcaaagag agtgaaacct gaggaagaag aagagagaga
     3481 catgaagaaa tctaggggat tcgatgaaag ggagttgttt tcgactgctg aatcttcttc
     3541 ttcttcgagt gtgtttttcg tttcgcagtc ttgctcgttg gcatcagaag ggaagaatct
     3601 ggaaggtatt caagattcat ctgatcagat tactacaagt ttgggaaaaa atggttgcaa
     3661 atgagcggcc gccaccgcgg tggagctcta agcaaatagc taaattatat acgaattaat
     3721 attatgatta agtgtttacg tgagtgcgat atttttatta ctatcttata cagttgtata
     3781 tactctataa aatgagttgt ctattaatta acgcgatgaa tgctttctgg gtttacctct
     3841 ccaacaactc tagtttactt ctcaatacat tcaattgtat ttgatttgtc aatacttcat
     3901 cattaatcaa ttctatagtt ttgtttttct cgtttatttc caaatttaat gcatcaattt
     3961 tattattcaa tttgtcgttg attttggtta atgattttat ggtttgatct ctggcattga
     4021 ttgtttgtgt tagtttttca ttattgataa ttaaattatt taagttagtt atcaactcgg
     4081 tgttttcaag tttcaagttt tcaatttctt tagagtttat tagatttgtc aaagtttctg
     4141 aattgcttga ttggtcctgt agaagagtat ttgttgttgt ggataattga ttcaattttt
     4201 gagacaattg ctggaaggcg ttgaaatatc tagcatcaat ctcatggttt ttttcccgag
     4261 agtctcgtag attcaattgt tttaatatat cttgggacca ctcttgattt gaactcatgg
     4321 aaattaaact gggtgttgtg ttgtggtgta atgattgtac cccctttgct tataattgtg
     4381 tggacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     4441 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     4501 acatcacatc cgaacataaa caaccatgac agtcaacact aagacctata gtgggagagc
     4561 agaaactcat gcctcaccag tagcacaacg attatttcga ttaatggaac tgaagaaaac
     4621 caatttatgt gcatcaattg atgttgatac cactaaggaa ttccttgaat taattgataa
     4681 attgggtcct tatgtatgct taatcaagac tcatattgat ataatcaatg atttttccta
     4741 tgaatccact attgaaccat tattagaact ttcacgtaaa catcaattta tgatttttga
     4801 agatagaaaa tttgctgata ttggtaatac cgtgaagaaa caatatattg gtggagttta
     4861 taaaattagt agttgggcag atattactaa tgctcatggt gtcactggga atggagtagt
     4921 tgaaggatta aaacagggag ctaaagaaac caccaccaac caagagccaa gagggttatt
     4981 gatgttagct gaattatcat cagtgggatc attagcatat ggagaatatt ctcaaaaaac
     5041 tgttgaaatt gctaaatccg ataaggaatt tgttattgga tttattgccc aacgtgatat
     5101 gggtggacaa gaagaaggat ttgattggct tattatgaca cctggagttg gattagatga
     5161 taaaggtgat ggattaggac aacaatatag aactgttgat gaagttgtta gcactggaac
     5221 tgatattatc attgttggta gaggattgtt tggtaaagga agagatccag atattgaagg
     5281 taaaaggtat agagatgctg gttggaatgc ttatttgaaa aagactggcc aattataatc
     5341 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     5401 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     5461 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     5521 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     5581 acgagctcga attcatcgac ccgggaatct cggtcgtaat gatttctata atgacgaaaa
     5641 aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat ccaatacctc
     5701 gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag acaggactgt
     5761 aaagatggac gcattgaact ccaaagaaca acaagagttc caaaaagtag tggaacaaaa
     5821 gcaaatgaag gatttcatgc gtttgtactc taatctggta gaaagatgtt tcacagactg
     5881 tgtcaatgac ttcacaacat caaagctaac caataaggaa caaacatgca tcatgaagtg
     5941 cgtttaaacg gccacgctgg ccccctatag tgagtcgtat tacaattcac tggccgtcgt
     6001 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6061 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6121 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6181 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6241 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6301 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6361 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6421 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6481 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6541 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6601 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     6661 aactgatata attaaattga agctctaatt tgtgagttta gtattaggat ctctaccctt
     6721 ggcgaaaagt cctctgccaa caatgatgat atctgatcca ccacttacaa cttcgtcgac
     6781 ggttctgtac tgctgaccca atatgcgtat atataccaat ctaagtctgt gctccttcct
     6841 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     6901 aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca
     6961 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     7141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcggcg
     7201 cgccgaggac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7261 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7321 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7381 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7441 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7501 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7561 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7621 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7681 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7741 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7801 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7861 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7921 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7981 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8041 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8101 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8161 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8221 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8281 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8461 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8521 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8581 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8641 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8701 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8761 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8821 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8881 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8941 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9001 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9061 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9121 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9181 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9241 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9301 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9361 atgaccatga ttacgccaag ctcggaatta accctcacta aagggaacaa aagctg
//
