LOCUS       sequence-341962-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway 
            JW, Conaway RC
  TITLE     Subunit organization of the human INO80 chromatin remodeling 
            complex: an evolutionarily conserved core complex catalyzes 
            ATP-dependent nucleosome remodeling.
  JOURNAL   J Biol Chem. 2011 Apr 1;286(13):11283-9. doi: 
            10.1074/jbc.M111.222505. Epub 2011 Feb 8.
  PUBMED    21303910
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.222505"; journalName: "J Biol Chem"; date: 
            "2011-04-1- 1"; volume: "286"; issue: "13"; pages: "11283-9"
COMMENT     Sequence Label: pcDNA5FRT/FLAG-Ino80 Delta-N-EQ  (267-1556)
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             673..696
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3657..3668
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4720..4737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4726..4950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5083..5102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5234..5281
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5289..6311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5289..6311
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6441..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6478..6497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6532..6551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6611..6627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6611..6627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6624..6646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6635..6651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6659..6665,6666..6683,6684..6689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6704..6725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6842..6859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7013..7601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7093..7112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7772..8563,8564..8632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8395..8414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8633..8737)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8812..8831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgc cagactacaa ggacgatgat gacaagctcg accacttatc cattgagcag
      721 ctgaatgctc gtcgcaggaa agtatggctc agcattgtga aaaaggaact accaaaggca
      781 aataagcaga aagcttcagc tcgtaacctg tttctcacca atagccgaaa gcttgctcac
      841 cagtgcatga aggaggtgcg tcgagctgcc ttgcaggccc agaagaactg taaggaaacc
      901 ttgcctcgtg cccgccgcct caccaaggag atgcttctgt actggaagaa atatgagaaa
      961 gtagagaagg agcaccgcaa gagagcagag aaggaagctt tggagcagcg gaagttggat
     1021 gaggaaatgc gggaggccaa gaggcaacag cgaaaactca acttcttaat tacccagaca
     1081 gagttgtatg cccatttcat gagtcgcaaa cgagatatgg gtcatgatgg tatccaggaa
     1141 gaaatcctaa ggaaactgga agacagttct acccagagac aaatcgatat aggtggagga
     1201 gtggtagtta acatcacaca ggaggattat gatagtaacc attttaaagc ccaggccctg
     1261 aagaatgctg aaaatgctta ccatattcac caagctcgga caaggtcatt tgatgaagat
     1321 gcaaaagaaa gtcgagcagc tgccctacgg gcagcaaaca agtctggcac tgggtttggg
     1381 gagagttata gcctggctaa cccatctatc cgggctggtg aggatattcc acagcccaca
     1441 atttttaatg gcaaattgaa aggttatcaa ctgaaaggca tgaattggtt ggccaatcta
     1501 tatgaacagg gtattaatgg cattcttgct gatgaaatgg gccttggtaa aacagtacag
     1561 agcattgccc ttctggccca tctggctgag agagagaaca tttggggacc tttcttaata
     1621 atttcacctg cgtctacact taacaattgg caccaggagt ttactagatt tgttcctaaa
     1681 tttaaggtgc taccatattg gggaaatcct catgatagaa aagtcatcag aaggttctgg
     1741 agtcagaaga ccctctacac tcaggatgcc cccttccatg tggttattac cagctatcag
     1801 ctggtggtgc aggatgtaaa gtatttccag cgggtcaagt ggcaatacat ggtactggat
     1861 caggctcagg cgctcaagag tagttccagt gttcgttgga agatcctctt acagttccag
     1921 tgtcggaatc ggcttttgct aaccgggacc ccaattcaga acaccatggc agagctttgg
     1981 gctctgctgc atttcattat gccaacatta tttgattcac atgaggaatt taatgaatgg
     2041 ttttccaagg acattgagag ccatgccgaa aacaaatctg ctattgatga gaatcaactt
     2101 tctcgcttac acatgatttt gaagccattt atgctgagga gaatcaagaa agatgtggaa
     2161 aatgaattat ctgacaagat tgagattcta atgtattgcc aactgaccag ccgacagaag
     2221 ctgctatatc aggcactaaa gaacaaaatt tccattgagg atttattgca gtcttctatg
     2281 ggctctaccc aacaagcaca gaacaccacc agcagcctca tgaatctggt catgcagttt
     2341 aggaaggtgt gtaatcaccc ggagttattt gaacggcaag aaacttggtc tccatttcat
     2401 atttccctaa agccatacca catttcaaag tttatctacc gtcatggaca gatcagggtc
     2461 ttcaatcatt cacgagacag gtggttaagg gttctttctc catttgcacc agactatatc
     2521 caacggtctc tctttcacag aaaaggtatt aatgaagaaa gctgtttctc tttccttcgc
     2581 tttattgata tatctccagc agaaatggca aaccttatgc ttcagggact tttggccaga
     2641 tggttagctc ttttcctgtc tctgaaagcc tcctacaggc tccatcagct acgctcctgg
     2701 ggagcgccag aaggggagag ccaccagaga tacctgagga acaaggattt ccttcttggg
     2761 gttaattttc cactctcctt tccaaacctt tgcagctgcc ctttgttaaa gtctcttgtt
     2821 ttcagcagcc actgtaaagc agtgagtggc tactcagacc aggttgtcca tcagcggaga
     2881 tcagctacct cctcgctgcg tcgctgcctg ctcactgagc tgccatcttt tttgtgtgtg
     2941 gccagtccac gagttaccgc agtgccattg gattcttact gcaatgaccg aagtgcagaa
     3001 tatgaaaggc gagttctgaa ggaaggaggg agtctggcag ccaagcagtg tttgttgaat
     3061 ggggcccctg aactggctgc agactggcta aatagacgat cacagttctt cccagagcca
     3121 gctggaggtc tgtggagcat cagacctcag aatggctggt ctttcatcag gattccaggc
     3181 aaggagagcc tcatcactga cagtggaaag ctgtatgccc ttgatgtcct gctgactcgg
     3241 ctcaagtctc aagggcatag ggtccttatc tactcccaga tgaccaggat gatagaccta
     3301 ctggaggaat acatggttta caggaagcat acctacatga ggcttgatgg ctcatccaag
     3361 atctcggaga ggcgagacat ggttgctgat tttcagaaca ggaatgacat ctttgtgttc
     3421 ctgttaagca cacgagctgg aggactgggt atcaatctca ctgctgcaga cacagtgatt
     3481 ttctatgata gcgactggaa ccccactgtg gaccagcagg ccatggacag ggcccaccgc
     3541 ttagggcaga caaagcaggt tactgtgtac cggctcatct gtaaaggcac cattgaagaa
     3601 cgcattctgc aaagagccaa ggagaagagt gagattcagc ggatggtgat ttcaggtggg
     3661 aacttcaaac cagatacctt gaaacccaaa gaggtggtta gtcttcttct agacgacgaa
     3721 gagttggaga agaaactgag gctgcggcag gaagagaaac ggcaacagga ggaaaccaac
     3781 cgagtgaaag agcgcaagcg gaagcgggaa aagtatgcag agaagaagaa aaaagaagat
     3841 gaattggatg ggaaaaggag aaaagagggt gtgaacctgg tgatcccatt tgttccctcg
     3901 gctgataact ccaacctctc tgctgacgga gatgactcct tcattagcgt ggactcagcc
     3961 atgccaagcc ctttcagtga gatctccatc agcagtgagc tgcacactgg ctccattccc
     4021 ctggacgaga gcagcagtga catgctggtc attgtggatg acccagcctc ctcagcccct
     4081 cagtctcgag ctaccaactc tcccgcatcc ataacaggct ccgtctcaga taccgtgaat
     4141 ggaatttcca ttcaggaaat gccagctgca ggacgtggtc actcagcccg aagccgaggc
     4201 cgccccaaag gttcaggaag cacagccaaa ggagcaggga agggccggag ccgaaagtcc
     4261 acggcaggca gtgctgctgc aatggcagga gccaaagccg gggctgcagc ggcctctgca
     4321 gctgcctatg ccgcatacgg gtacaacgtg tctaaaggaa tctccgccag cagtcctctg
     4381 cagacatccc ttgttcggcc tgctggcctt gctgactttg gaccttcaag cgcctcttct
     4441 cctttgagtt cccctttgag caagggaaat aatgttcctg ggaatcccaa gaacctccac
     4501 atgaccagca gcctagcccc agactctctg gtccggaaac agggcaaagg caccaacccc
     4561 tctggaggac ggtaagcggc cgcagaaaca agggcgaatt ccagcacact ggcggccgtt
     4621 actagtggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
     4681 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4741 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4801 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4861 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4921 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4981 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5041 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5101 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5161 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5221 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     5281 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     5341 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     5401 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     5461 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     5521 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     5581 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     5641 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     5701 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     5761 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     5821 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     5881 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     5941 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     6001 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     6061 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     6121 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     6181 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     6241 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     6301 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6361 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6421 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6541 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8761 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     8821 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8881 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     8941 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     9001 tt
//
