LOCUS       sequence-341965-        9193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV53 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV53 L1L2
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3110..3231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3121..3140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3175..3194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3419..4007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3908..3927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4087..4390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4505..5810
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4847..5778
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5829..5838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5833..5842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7236..7680
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7713..7759
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7751..7769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7779..8573
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7779..8573
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7833..7852)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8443..8462
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8620..9192
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccgtgtgga ggcccagcga cagcaaggtg
     1621 tacctgcccc ccacccccgt gagcaaggtg atcaccaccg acgcctacgt gaagaggacc
     1681 accatcttct accacgccgg cagcagcagg ctgctgaccg tgggccaccc ctactacccc
     1741 atcagcaaga gcggcaaggc cgacatcccc aaggtgagcg ccttccagta cagggtgttc
     1801 agggtgaggc tgcccgaccc caacaagttc ggcctgcccg acaccaacat cttcaacccc
     1861 gaccaggaga ggctggtgtg ggcctgcgtg ggcctggaga tcggcagggg ccagcccctg
     1921 ggcgtgggcg tgagcggcca ccccctgttc aacaggctgg acgacaccga gagcagcagc
     1981 atcgccatcc aggacaccgc ccccgacagc agggacaacg tgagcgtgga ccccaagcag
     2041 acccagctgt gcatcatcgg ctgcgccccc gccatcggcg agcactggac caagggcacc
     2101 gcctgcagga gcacccccac caccgccggc gactgccccc ccctggagct gatcaacagc
     2161 cccatcgagg acggcgacat ggtggacacc ggcttcggcg ccctgaactt caaggccctg
     2221 caggagagca agagcgacgt gcccctggac atcgtgcaga gcacctgcaa gtaccccgac
     2281 tacctgaaga tgagcgccga cgcctacggc gacagcatgt ggttctacct gaggagggag
     2341 cagctgttca ccaggcactt cttcaacagg gccggcgtga tcggcgagga gatccccaac
     2401 gacctgtaca tcaagggcag caacggcagg gacccccccc ccagcagcgt gtacgtggcc
     2461 acccccagcg gcagcatgat caccagcgag gcccagctgt tcaacaagcc ctactggctg
     2521 cagagggccc agggccacaa caacggcatc tgctggaaca accagctgtt cgtgaccgtg
     2581 gtggacacca ccaggaacac caacatgacc ctgagcgcca ccacccagag catgagcacc
     2641 tacaacagca agcagatcaa gcagtacgtg aggcacgccg aggagtacga gctgcagttc
     2701 gtgttccagc tgtgcaagat cagcctgagc gccgaggtga tggcctacct gcacaccatg
     2761 aacagcaccc tgctggagga ctggaacatc ggcctgagcc cccccgtggc caccagcctg
     2821 gaggacaagt acaggtacgt gaagagcgcc gccatcacct gccagaagga ccagcccccc
     2881 cccgagaagc aggaccccct gagcaagtac aagttctggg aggtgaacct gcagaacagc
     2941 ttcagcgccg acctggacca gttccccctg ggcaggaagt tcctgatgca ggtgggcgtg
     3001 aggaccaagc cccccgtgag cagcaagaag aggagcgcca gcaccaccag caccagcgcc
     3061 cccagcagca agaggaagag gaagtaatag tctagctggc cagacatgat aagatacatt
     3121 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3181 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3241 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     3301 taaaacctct acaaatgtgg tatggaaatg ttaattaact agccatgacc aaaatccctt
     3361 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3421 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3481 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3541 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3601 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3661 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3721 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3781 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3841 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3901 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3961 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4021 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc ttaattaacc
     4081 tgcaggcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4381 tattaccatg atgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4441 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt gactagtgga
     4501 gccgagagta attcatacaa aaggagggat cgccttcgca aggggagagc ccagggaccg
     4561 tccctaaatt ctcacagacc caaatccctg tagccgcccc acgacagcgc gaggagcatg
     4621 cgcccagggc tgagcgcggg tagatcagag cacacaagct cacagtcccc ggcggtgggg
     4681 ggaggggcgc gctgagcggg ggccagggag ctggcgcggg gcaaactggg aaagtggtgt
     4741 cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa cggtatataa gtgcggtagt
     4801 cgccttggac gttctttttc gcaacgggtt tgccgtcaga acgcaggtga gtggcgggtg
     4861 tggcttccgc gggccccgga gctggagccc tgctctgagc gggccgggct gatatgcgag
     4921 tgtcgtccgc agggtttagc tgtgagcatt cccacttcga gtggcgggcg gtgcgggggt
     4981 gagagtgcga ggcctagcgg caaccccgta gcctcgcctc gtgtccggct tgaggcctag
     5041 cgtggtgtcc gccgccgcgt gccactccgg ccgcactatg cgttttttgt ccttgctgcc
     5101 ctcgattgcc ttccagcagc atgggctaac aaagggaggg tgtggggctc actcttaagg
     5161 agcccatgaa gcttacgttg gataggaatg gaagggcagg aggggcgact ggggcccgcc
     5221 cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgtgg ctttccgaag
     5281 caatcgggcg tgagtttagc ctacctgggc catgtggccc tagcactggg cacggtctgg
     5341 cctggcggtg ccgcgttccc ttgcctccca acaagggtga ggccgtcccg cccggcacca
     5401 gttgcttgcg cggaaagatg gccgctcccg gggccctgtt gcaaggagct caaaatggag
     5461 gacgcggcag cccggtggag cgggcgggtg agtcacccac acaaaggaag agggccttgc
     5521 ccctcgccgg ccgctgcttc ctgtgacccc gtggtctatc ggccgcatag tcacctcggg
     5581 cttctcttga gcaccgctcg tcgcggcggg gggaggggat ctaatggcgt tggagtttgt
     5641 tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca ttcttggaat
     5701 ttgccccttt gagtttggag cgaggctaat tctcaagcct cttagcggtt caaaggtatt
     5761 ttctaaaccc gtttccaggt gttgtgaaag ccaccgctaa ttcaaagcaa tccggagtat
     5821 acggatccgc caccatggtg gcccacaggg ccaggcggag gaagagggcc agcgccaccc
     5881 agctgtacca gacctgcaag cagagcggca cctgccccga ggacgtgatc aacaagatcg
     5941 agcacaagac ctgggccgac aagatcctgc agtggggcag cctgttcacc ttcttcggcg
     6001 gcctgggcat cggcaccggc agcggcaccg gcggcaggac cggctacatc cccctgggca
     6061 ccaggcccag caccgtggtg gacgtgaccc ccgccaggcc ccccatcgtg gtggagagcg
     6121 tgggccccac cgaccccagc atcgtgaccc tggtggagga gagcagcgtg atcgagagcg
     6181 gcgccagctt ccccaacttc accggcaccg ccggcttcga ggtgaccagc agcagcacca
     6241 ccacccccgc cgtgctggac atcacaccca ccagcaccag cgtgcacgtg agcagcacca
     6301 cctacaccaa ccccaccttc gtggaccccc ccgtgatcga ggtgccccag accggcgagg
     6361 tgagcggcaa catcctgatc agcaccccca ccagcggcgt gcacagctac gaggagatcc
     6421 ccatgcagac cttcgccgtg cagggcaccg gcaacgagcc catcagcagc acccccatcc
     6481 ccggcctgag gaggatcgcc gcccccaggc tgtacaagaa ggccttccag caggtgaagg
     6541 tgaccgaccc cgccttcctg cacaagcccg agaccctgat caacgtggac aaccccatct
     6601 tccagaacgc cgacaccacc ctgaccttca gccccagcgg cgtggccccc gaccccgact
     6661 tcctggacat cgtggccctg cacaggcccg ccttcaccac caggaggggc ggcgtgaggt
     6721 tcagcaggct gggcaccaag gccaccatga ggaccaggag cggcaagcag atcggcgcca
     6781 gggtgcacta ctactacgac gtgagcccca tcacccagac cgaggagatc gagatgcagc
     6841 ccctgctgag caccgacaac accttcgacg gcctgtacga catctacgcc aacatcgacg
     6901 acgaggcccc cgtgagcagc aggttcagca tcgccacccc cagcaggctg cccaccaaca
     6961 ccgtgcccct gagcttcagc ggcagcacca gcaacgtgac catccccttc ggcaccagct
     7021 gggacgtgcc catctacagc ggccccgacg tggtgctgcc caccggcccc cccacctggc
     7081 cctacgcccc ccagagcccc ttcgacacca cccacgacgt ggtgatccag ggcagcacct
     7141 tcgccctgtg gcccgtgtac ttcctgaaga ggaggaggag gaagaggatt ccctacttcc
     7201 tggccgacgg cggcgtggcc gcctaatagt ctaggagcag gtttccccaa tgacacaaaa
     7261 cgtgcaactt gaaactccgc ctggtctttc caggtctaga ggggtaacac tttgtactgc
     7321 gtttggctcc acgctcgatc cactggcgag tgttagtaac agcactgttg cttcgtagcg
     7381 gagcatgacg gccgtgggaa ctcctccttg gtaacaagga cccacggggc caaaagccac
     7441 gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg actttactgc gaaacccact
     7501 ttaaagtgac attgaaactg gtacccacac actggtgaca ggctaaggat gcccttcagg
     7561 taccccgagg taacacgcga cactcgggat ctgagaaggg gactggggct tctataaaag
     7621 cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg gaggtcggca cctttccttt
     7681 gcaattactg accctatgaa tacactgact gtttgacaat taatcatcgg catagtatat
     7741 cggcatagta taatacgact cactatagga gggccaccat gattgaacaa gatggattgc
     7801 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7861 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7921 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     7981 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     8041 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8101 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8161 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8221 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8281 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgacac
     8341 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8401 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8461 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8521 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8581 tctggggttc gaaatgaccg accaagcgaa ttcgctagga ttatccctaa tacctgccac
     8641 cccactctta atcagtggtg gaagaacggt ctcagaactg tttgtttcaa ttggccattt
     8701 aagtttagta gtaaaagact ggttaatgat aacaatgcat cgtaaaacct tcagaaggaa
     8761 aggagaatgt tttgtggacc actttggttt tcttttttgc gtgtggcagt tttaagttat
     8821 tagtttttaa aatcagtact ttttaatgga aacaacttga ccaaaaattt gtcacagaat
     8881 tttgagaccc attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt caacaccgag
     8941 acatttaggt gaaagacatc taattctggt tttacgaatc tggaaacttc ttgaaaatgt
     9001 aattcttgag ttaacacttc tgggtggaga atagggttgt tttcccccca cataattgga
     9061 aggggaagga atatcattta aagctatggg agggttgctt tgattacaac actggagaga
     9121 aatgcagcat gttgctgatt gcctgtcact aaaacaggcc aaaaactgag tccttgggtt
     9181 gcatagaaag ctg
//
