LOCUS       sequence-341967-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV70 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV70 L1L2
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3125..3246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3136..3155)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3190..3209
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3434..4022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3923..3942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4102..4405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4520..5825
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4862..5793
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5844..5853
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7260..7704
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7737..7783
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7775..7793
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7803..8597
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7803..8597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7857..7876)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8467..8486
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8644..9216
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccctgtgga ggagcagcga caacaccgtg
     1621 tacctgcccc ctcccagcgt ggccaaggtg gtcaacaccg acgactacgt gaccaggacc
     1681 ggcatctact actacgccgg cagcagcagg ctgctgaccg tgggccaccc ctacttcaag
     1741 gtgcccgtga acggcggcag gaagcaggag atccccaagg tgagcgccta ccagtacagg
     1801 gtgttcaggg tgagcctccc cgaccccaac aagttcggcc tgcccgaccc cagcctgtac
     1861 aaccccgaca cccagaggct ggtgtgggcc tgcatcggcg tggagatcgg caggggccag
     1921 cccctgggcg tgggcgtgag cggccacccc ctgtacaaca ggctggacga caccgagaac
     1981 agccacttca gcagcgccgt gagcacccag gacagcaggg acaacgtgag cgtggactac
     2041 aagcagaccc agctgtgcat catcggctgc gtgcccgcca tgggcgagca ctgggccaag
     2101 ggcaaggcct gcaagagcac ccagcagggc gactgccccc ccctggagct ggtgaacacc
     2161 gccatcgagg acggcgacat gatcgacacc ggctacggcg ccatggactt caggaccctg
     2221 caggagacca agagcgaggt gcccctggac atctgccaga gcgtgtgcaa gtaccccgac
     2281 tacctgcaga tgagcgccga cgtgtacggc gacagcatgt tcttctgcct gaggaaggag
     2341 cagctgttcg ccaggcactt ctggaacagg ggcggcatgg tgggcgacac catccccagc
     2401 gagctgtaca tcaagggcac cgacatcagg gagaggcccg gcacccacgt ctacagcccc
     2461 agccccagcg gcagcatggt gagcagcgac agccagctgt tcaacaagcc ctactggctg
     2521 cacaaggccc agggccacaa caacggcatc tgctggcaca accagctgtt catcaccgtg
     2581 gtggacacca ccaggagcac caacttcacc ctgagcgcct gcaccgagac cgccatcccc
     2641 gccgtgtaca gccccaccaa gttcaaggag tacaccaggc acgtggagga gtacgacctg
     2701 cagttcatct tccagctgtg caccatcacc ctgaccgccg acgtgatggc ctacatccac
     2761 accatgaacc ccgccatcct ggacaactgg aacatcggcg tgaccccccc ccccagcgcc
     2821 agcctggtgg acacctacag gtacctgcag agcgccgcca tcgcctgcca gaaggacgcc
     2881 cccacccccg agaagaagga cccctacgac gacctgaagt tctggaacgt ggacctgaag
     2941 gagaagttca gcaccgagct ggaccagttc cccctgggca ggaagttcct gctgcaggtg
     3001 ggcgccagga ggaggcccac catcggcccc aggaagaggc ccgccagcgc caagagcagc
     3061 agcagcgcca gcaagcacaa gaggaagagg gtgagcaagt aatagtctag ctggccagac
     3121 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3181 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3241 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     3301 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg aaatgttaat taactagcca
     3361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4081 tgttcttaat taacctgcag gcgttacata acttacggta aatggcccgc ctggctgacc
     4141 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4201 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4261 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4321 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4381 cgtattagtc atcgctatta ccatgatgat gcggttttgg cagtacatca atgggcgtgg
     4441 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4501 gttttgacta gtggagccga gagtaattca tacaaaagga gggatcgcct tcgcaagggg
     4561 agagcccagg gaccgtccct aaattctcac agacccaaat ccctgtagcc gccccacgac
     4621 agcgcgagga gcatgcgccc agggctgagc gcgggtagat cagagcacac aagctcacag
     4681 tccccggcgg tggggggagg ggcgcgctga gcgggggcca gggagctggc gcggggcaaa
     4741 ctgggaaagt ggtgtcgtgt gctggctccg ccctcttccc gagggtgggg gagaacggta
     4801 tataagtgcg gtagtcgcct tggacgttct ttttcgcaac gggtttgccg tcagaacgca
     4861 ggtgagtggc gggtgtggct tccgcgggcc ccggagctgg agccctgctc tgagcgggcc
     4921 gggctgatat gcgagtgtcg tccgcagggt ttagctgtga gcattcccac ttcgagtggc
     4981 gggcggtgcg ggggtgagag tgcgaggcct agcggcaacc ccgtagcctc gcctcgtgtc
     5041 cggcttgagg cctagcgtgg tgtccgccgc cgcgtgccac tccggccgca ctatgcgttt
     5101 tttgtccttg ctgccctcga ttgccttcca gcagcatggg ctaacaaagg gagggtgtgg
     5161 ggctcactct taaggagccc atgaagctta cgttggatag gaatggaagg gcaggagggg
     5221 cgactggggc ccgcccgcct tcggagcaca tgtccgacgc cacctggatg gggcgaggcc
     5281 tgtggctttc cgaagcaatc gggcgtgagt ttagcctacc tgggccatgt ggccctagca
     5341 ctgggcacgg tctggcctgg cggtgccgcg ttcccttgcc tcccaacaag ggtgaggccg
     5401 tcccgcccgg caccagttgc ttgcgcggaa agatggccgc tcccggggcc ctgttgcaag
     5461 gagctcaaaa tggaggacgc ggcagcccgg tggagcgggc gggtgagtca cccacacaaa
     5521 ggaagagggc cttgcccctc gccggccgct gcttcctgtg accccgtggt ctatcggccg
     5581 catagtcacc tcgggcttct cttgagcacc gctcgtcgcg gcggggggag gggatctaat
     5641 ggcgttggag tttgttcaca tttggtgggt ggagactagt caggccagcc tggcgctgga
     5701 agtcattctt ggaatttgcc cctttgagtt tggagcgagg ctaattctca agcctcttag
     5761 cggttcaaag gtattttcta aacccgtttc caggtgttgt gaaagccacc gctaattcaa
     5821 agcaatccgg agtatacgga tccgccacca tggtgagcag cagggccagc aggaggaaga
     5881 gggccagcgc caccgacatc tacaagacct gcaagcagag cggcacctgc ccccccgacg
     5941 tggtgaacaa ggtggagggc accaccctgg ccgacaggtt cctgcagtgg gccagcctgg
     6001 gcatcttcct gggcggcctg ggcatcggga ccgggaccgg caccggcggc aggaccggct
     6061 acatccccct gggcggcagg cctagcaccg tggtggacgt gacccccgcc aggccccccg
     6121 tggtgatcga gcccgtgggc cccaccgagc ccagcatcgt gcagctggtg gaggagagca
     6181 gcgtggtgag cagcggcacc cccatcccca ccttcaccgg caccagcggc ttcgagatca
     6241 ccagcagcgc caccaccacc cccgccgtgc tggacatcac ccccgccagc ggcagcgtgc
     6301 agatcagcac caccagctac accaatcccg ccttcgccga ccccagcctg atcgaggtgc
     6361 cccagaccgg cgaggtgagc ggcaacatct tcgtgaccac ccccaccagc ggcacccacg
     6421 gctacgagga gatccccatg caggtgttcg ccagccacgg caccggcacc gagcccatca
     6481 gcagcacccc cgtgcccggc gtgagcaggg tggccggccc caggctgtac agcagggcct
     6541 accaccaggt gagggtgaac aacttcgact tcgtgaccag gcccagcagc ttcgtgacct
     6601 tcgacaaccc cgcctttgag cccggcgaca ccagcctgac cttcgagccc gccgacaccg
     6661 cccccgaccc cgacttcctg gacatcgtga ggctgcacag gcccgccctg accagccgga
     6721 ggggcaccgt gaggttcagc aggctgggca agaaggccac catgttcacc aggaggggca
     6781 cccagatcgg cgcccaggtg cactactacc acgacatcag caacatcacc gccaccgagg
     6841 acatcgagat gcagcccctg ctgaccagcg agagcaccga cggcctgtac gacatctacg
     6901 ccgacgccga catcgacaac gccatgctgc acaccaccag ccacaccggc agcaccggcc
     6961 ccaggagcca cctgagcttc cccagcatcc ccagcaccgt gagcaccaag tacagcaaca
     7021 ccaccatccc cttcaccacc agctgggaca tccccgtgac caccggcccc gacatcgtgc
     7081 tgcccaccgc cagccccaac ctgcccttcg tgccccccac cagcatcgac accaccgtgg
     7141 ccatcgccat ccagggcagc aactactacc tgctgcccct gctgtactac ttcctgaaga
     7201 agaggaagag gattccctac ttcttcaccg acggcttcgt ggccgtgtaa tagtctagga
     7261 gcaggtttcc ccaatgacac aaaacgtgca acttgaaact ccgcctggtc tttccaggtc
     7321 tagaggggta acactttgta ctgcgtttgg ctccacgctc gatccactgg cgagtgttag
     7381 taacagcact gttgcttcgt agcggagcat gacggccgtg ggaactcctc cttggtaaca
     7441 aggacccacg gggccaaaag ccacgcccac acgggcccgt catgtgtgca accccagcac
     7501 ggcgacttta ctgcgaaacc cactttaaag tgacattgaa actggtaccc acacactggt
     7561 gacaggctaa ggatgccctt caggtacccc gaggtaacac gcgacactcg ggatctgaga
     7621 aggggactgg ggcttctata aaagcgctcg gtttaaaaag cttctatgcc tgaataggtg
     7681 accggaggtc ggcacctttc ctttgcaatt actgacccta tgaatacact gactgtttga
     7741 caattaatca tcggcatagt atatcggcat agtataatac gactcactat aggagggcca
     7801 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7861 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7921 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7981 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8041 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8101 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8161 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8221 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8281 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     8341 acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc atggtggaaa
     8401 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8461 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8521 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8581 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgaattcgct
     8641 aggattatcc ctaatacctg ccaccccact cttaatcagt ggtggaagaa cggtctcaga
     8701 actgtttgtt tcaattggcc atttaagttt agtagtaaaa gactggttaa tgataacaat
     8761 gcatcgtaaa accttcagaa ggaaaggaga atgttttgtg gaccactttg gttttctttt
     8821 ttgcgtgtgg cagttttaag ttattagttt ttaaaatcag tactttttaa tggaaacaac
     8881 ttgaccaaaa atttgtcaca gaattttgag acccattaaa aaagttaaat gagaaacctg
     8941 tgtgttcctt tggtcaacac cgagacattt aggtgaaaga catctaattc tggttttacg
     9001 aatctggaaa cttcttgaaa atgtaattct tgagttaaca cttctgggtg gagaataggg
     9061 ttgttttccc cccacataat tggaagggga aggaatatca tttaaagcta tgggagggtt
     9121 gctttgatta caacactgga gagaaatgca gcatgttgct gattgcctgt cactaaaaca
     9181 ggccaaaaac tgagtccttg ggttgcatag aaagctg
//
