LOCUS       sequence-341977-       13104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous zebrafish expression vector containing secreted human 
            AnnexinV tagged to mKate2. Parton lab clone BDN..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actb2-SecAnxaV-mKate2-acry-EGFP
FEATURES             Location/Qualifiers
     source          1..13104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1056..1177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1300..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1303..2013,2014..2016,2017..2019))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1345..1366)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1694..1713
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1953..1974
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2016..2025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2667..2683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2703..2723
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8030..8054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8055..8064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9076..9100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9102..9797
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    9919..10000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10001..10021)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10029..10045)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10061..10195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(10634..10821,10822..10833)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10867..10883)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10867..10883)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10920..10940)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10920..10938)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10959..10975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10972..10994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10983..10999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11007..11013,11014..11031,11032..11037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11052..11073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11190..11207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11361..11949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11441..11460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12120..12911,12912..12980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12743..12762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12981..13085)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatt ggccttaaga tacattgatg agtttggaca
     1081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1141 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1201 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1261 atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat
     1321 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     1381 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     1441 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     1501 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     1561 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     1621 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     1681 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     1741 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     1801 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     1861 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     1921 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     1981 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtaatgtcag
     2041 acctggtaac tccttactgt aacttattgc acctgatgaa caaaagatgt gaactgaacg
     2101 caagtaggag gctaatagag gctagtacag tacaaagatc cccaaatgat gtgtgccggc
     2161 cttagtggag gcagacccct ggttatatac ctgtgctgca gaaagagggg cttacaaatg
     2221 gatctctctg gaatggcatt atctcatggt gggaacagtg ttgtcagcag aatcagcggg
     2281 gtggactctg acctgagaat gacagtctag cactgaatgg ctcagacaat actcttgtgg
     2341 ccaactatca ccggaaaata agtgcctgcc aaaacaaacc agaccagatg aagctacatg
     2401 gctgtagaac gtcagtattt aatgtgttgt ggccacttgg ttcaccagat ttaagagcta
     2461 atttttttaa atatcacaga taatcaatcg cttttttttt tcttgactga aaagcttaga
     2521 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
     2581 aatgcacact attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat
     2641 tctgtgcatt tcacaatttt ctcgagcagg aaacagctat gaccatgatt acgccaagct
     2701 atcaactttg tatagaaaag ttgaaaattc cagtttgaag aaacttttca agactcacct
     2761 tttcactgtt ggtcttaatt taggttgatt atttttgtca atttaataat tacattgtat
     2821 tgttcatatt ttattgtctt attgtggtgt ttttacagct tttttattgt tctgctttgt
     2881 tactgtttgc cttgtatgca ttgtagcgct ttgggtctga gaaaagcgca ctataaataa
     2941 actgtattat tattattagg gatggacatc acggtggcgc gatgggtggc acaattacct
     3001 cacagcaagt aggttgctgt ttagagcccc ggctggatca gttggcagtt ctgtgtggag
     3061 tttgcatgtt ctccctgttc aagtaggttt cctcccggtg ctctggtttc ccctcacaag
     3121 tccaaagaca tgatgtatag gtgaatgggg taagctaaat tgaccatagt gagtgtgtgt
     3181 gtgtgtgtga atgagagtgt ataggtgttt cccagtgtta ggttgcaact ggaaggacat
     3241 ctgctgtgta aaacatatgc aggataagtt ggcggttcat tcccctatgg tgacccctgg
     3301 ttaattaaag gactaagttg aaaatgaatg aatgaatgaa tgattagaga taaaccgata
     3361 ccactttttt acaaaccgat atgagtacaa gtataatagt ttctgttact cgacaatact
     3421 gagtaccgat tctgatactt cttggatttg gtttcaatgt aattaatttg aaagaaggtt
     3481 ttatttcttg cctgtcgcaa ggatgaacac aaaaatcttt accagaagga tattttaagc
     3541 taagaatata cgcacgttgt tgacaaccct gtatcgatta ccgattaaac gtggacattt
     3601 aaaggataag ttatgcttgc gccatcacaa ctttacacat tacatgatca aagatcatat
     3661 tgaatattcg gtttattcgt cagaagccta tagatttgtt aaaagtgtat tttataaacg
     3721 gatggtttat aaagactaag ctattaatat atcagacatt cattaataca tcaagacatc
     3781 gatgtcaggt aggacaacaa atcacatcca tgtaatgaaa tcttgttgag tggtattggt
     3841 gcgtggtttc agcatttcat gtgcaatacg agtaagaata ttttaaccaa gtatcgaccc
     3901 aaaacctgat actggtatcg gtgcatccct tattattatt attatcaaaa acacacttgt
     3961 tttatttatg tgggtgccaa acaacaaact atgaaatgca gcatgacacg cccaaaaagt
     4021 gttcctttga gaacaacttc ataaatatgt ttcttatcat tatgcatgaa tggtgtactg
     4081 ggagagcttt ggtatcagaa ttgagataag agactttggc attctaaagg acaatgacat
     4141 tatcacactg acagtcaaca acactttatt gctctggaaa tcttatgtca tggggcaaag
     4201 ctgaggttca ttaggacagt accttattta gagatgaatg ggaaggctgt agtgggctgc
     4261 tatcagggaa gggtggtggt cctcggggga actatggact cacaaaagca catctgctgt
     4321 aacaagatcc cctgtgcaat ctacaaaagt tacaaagaga agcatggttg gttaaaaaat
     4381 agaattgact ggatatgctg aatgcattgt ttgttgtgtg ttacatcaca ttaaccacag
     4441 attggtgcta aatatttgct tatcttataa aaacctgtgt gtttataaag gggggtgtgg
     4501 atggatgctt ggcattcttt gaaaagatgt tgggggatgg aaggaggaga ccttattgat
     4561 gagtatccag tttgcagata acaagttttt agacattcat actatgatgc tgtttatgtc
     4621 tgttttttct cattttttct atacacaaac agctataaaa cattcacttt tatatctgat
     4681 gctgtatatt aaatttcgtt gtatttattc tgtttgctct gttgggtcat agctagggcc
     4741 agacagaatc tgctgacatt ttttgctttt tctgcagaga attttgtaaa caatctgtga
     4801 tttatgcgag attttttcgg gagtatcgta actaaaaact tactatatga aataaaaaaa
     4861 aataatacct ttttaacttt taataaatat atacaatgca agtccaccta gatccaatta
     4921 tttggtcgac aaagcaagtc tctcacataa tatatcttct aaaagacaga aaacattact
     4981 ttcagaccat attgtaatca aatgaacact ttcatattag tcaataatat tacggaaatg
     5041 tatttaaaaa ctgaataaac atatatttac acctttgtct ttatttctag agtgctttta
     5101 caatgtagat tgtgtcaaag cagcttaact tagaggatta gtatagtaaa ttgaaactgt
     5161 gtcagtccat tttttaagag tttaagttca gttcagttta gttcagtgtg agttaatttt
     5221 tactgctgag agtccaaaca ctaaagagca aatccaaata cacaagtcaa taaataaact
     5281 caatgatggg ctaaagatct ggggaaatct gcagatttct gtgcgcacag attctgtgtg
     5341 ggcctagtca ttacctatac tcaacgttta ctctgagaaa cttccatatt ttagaggtaa
     5401 aatgctcaga attgtaaaaa gtcttgtcaa aatacttgga atttgccaaa aacaaaataa
     5461 aagtcacatc acctcattgt ccttggtatc agcaagcact tttaacacaa tcagctgttt
     5521 ttgtttataa ctcccactga aggccatgtg cattactccc aagttacaca gtaaaaagtc
     5581 acatgtagtt tatcatgatc actgctgctc aagcacagct ttatctgtta ttcagtgtgg
     5641 caatatctga aatgtgtgca aaatattatt tattgtagct gatcaccaac actttcagtg
     5701 tttttttttt ctacttttaa tgagaattat gttcttagga tttatcctta taaacctctt
     5761 taaaaagaaa tgttatgtct gcatagacac gacatgaaat tatgaacaga aaatttaact
     5821 atttaagatc aagacttgac atataacatt tctaatgaac attaaacctg aggtaggccc
     5881 acttgcaaaa atgtaactgc tatcactcac tttatacatc attgtgtaac ttgtacacac
     5941 aaaaatagtg aagcaatgtg gcatggaatg caggtcccat tcattcaaag ttattaaagc
     6001 actttcttaa agtgcattac tactgagcta agaccagcta gttgctcata caacatacta
     6061 caagtagtgt catcctaatt tggataaaaa aaatgtccat tttatacatg gaaaagtact
     6121 cattagatcc cccattgttt gtattacggt atttcgtgaa cacaagaggt aaatgaccta
     6181 cagagctgct gttgtgttag attgaaaaca caacacagga tcatggaggc atccatcatt
     6241 atgacgcatt ctgccacttg taactctgca cagacttttt gaaaagttta attgagtctt
     6301 ggcgttgtcg cagaaaaata cgaaaaattc tgtgttaaaa tttgcaaata aaaattccag
     6361 ttttatagga aatcgccatg gccttgttct ttaaccagtt aagcttcccc ttctttcact
     6421 ctcaagttgc aagaagcaag tgtagcaatg tgcacgcgac agccgggtgt gtgacgctgg
     6481 accaatcaga gcacagagct ccgaaagttt accttttatg gctagagccg ggtatgtgcc
     6541 gtcatataaa agagcgcgcc cagcttttca gcctcacttt gagctcctcc acacgcagct
     6601 agtgcggaat atcatctgct tgtaacccat tctcttaagt cgacaacccc ccaaacccaa
     6661 ggtgagttgg ttttacagtt tttaagcgtt taattatagt ttctatctat taatgttaag
     6721 taaaatgtgt aaatggataa atatgatttg atgaagtctt tagacagttc ataacggtct
     6781 ctttgtagtt atctactagg tttgatttat aagtggtggt tcttttgata atctatgtta
     6841 catttaataa atattggctg tatcttaact tttttgtagc gttagtggat gtggcaggtg
     6901 agaatgtcgg taatttaacg tgaccagtcg taggcacgac attgaacatt gaataggccg
     6961 gtgtgaaatg ggtgttaagt cttaatataa agttgtgttc tggctaatct ctctttaaca
     7021 gccataaatg tgatttttaa ctacagtttt gagggtgctg gtgagcatgt tgcacacttg
     7081 atggatagcc ggcatgggaa gttctttgtg caggcagtgc tgcagcaggg tgtgacctac
     7141 tttagctagc cggctaacca gctctcatct gatgtaacgt aaaccccatc aatctaacat
     7201 cggctcattc gtgctttata agatcgaaga tcttgttatt agtaacgggt attggcattt
     7261 agttttaact tggttaattt tctttttgtt atgtgttcta tttaaatgta gcctgcagcc
     7321 ccctctgtct ttaatgaagc tgaagattaa cgttaacgtt agtgaagtgt tgtatccgct
     7381 atgaaagatt tgaattttag aggtgtggtc cactttaata acggctgcgg agttctctgg
     7441 ctgcacttca gccgctgtga ctgtcgaagc agagcccatc agtttttcag gatctcggtg
     7501 atttttgagg ctagactatg aactgaaccg actgagagtg accgtccaga aatgctttag
     7561 tgtgaagtct ccgctcacga ggagtccgtg gactgactgc agatctgtga cgcagtaaca
     7621 cccggcggca gacacccgtt gaaatgcggt tgtgtaattg atcgcaggcg agggtggaag
     7681 aggatgtgaa acttcatttg tgtaaaattt agggagtggt cctggtgtga tgaatgtcga
     7741 aatctgttcc tttttactga gccctacgac tctggctgag tgccacaccg ccggcagccg
     7801 caaagcggct caaaccattg ccttttatgg taataatgag agaatgcaga gggacttcct
     7861 ttgtctggca tatctgaggc gcgcgctgtc actagcgccc accagcggtc agactgtaga
     7921 atgcagagca aaacaggaag ttgactccag atggtcacat gcttgctgaa acgcctctcc
     7981 ctggcagcag tgcaattcta acagtttccc tttcttttac agttcagcct caagtttgta
     8041 caaaaaagca ggctgccacc atggtgcaca aggtgctgct ggccctgttc ttcatcttcc
     8101 tggcccccgc cggcaccgca caggttctca gaggcactgt gactgacttc cctggatttg
     8161 atgagcgggc tgatgcagaa actcttcgga aggctatgaa aggcttgggc acagatgagg
     8221 agagcatcct gactctgttg acatcccgaa gtaatgctca gcgccaggaa atctctgcag
     8281 cttttaagac tctgtttggc agggatcttc tggatgacct gaaatcagaa ctaactggaa
     8341 aatttgaaaa attaattgtg gctctgatga aaccctctcg gctttatgat gcttatgaac
     8401 tgaaacatgc cttgaaggga gctggaacaa atgaaaaagt actgacagaa attattgctt
     8461 caaggacacc tgaagaactg agagccatca aacaagttta tgaagaagaa tatggctcaa
     8521 gcctggaaga tgacgtggtg ggggacactt cagggtacta ccagcggatg ttggtggttc
     8581 tccttcaggc taacagagac cctgatgctg gaattgatga agctcaagtt gaacaagatg
     8641 ctcaggcttt atttcaggct ggagaactta aatgggggac agatgaagaa aagtttatca
     8701 ccatctttgg aacacgaagt gtgtctcatt tgagaaaggt gtttgacaag tacatgacta
     8761 tatcaggatt tcaaattgag gaaaccattg accgcgagac ttctggcaat ttagagcaac
     8821 tactccttgc tgttgtgaaa tctattcgaa gtatacctgc ctaccttgca gagaccctct
     8881 attatgctat gaagggagct gggacagatg atcataccct catcagagtc atggtttcca
     8941 ggagtgagat tgatctgttt aacatcagga aggagtttag gaagaatttt gccacctctc
     9001 tttattccat gattaaggga gatacatctg gggactataa gaaagctctt ctgctgctct
     9061 gtggagaaga tgactaccca gctttcttgt acaaagtggc tgtgagcgag ctgattaagg
     9121 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     9181 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg
     9241 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     9301 aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccccgagg
     9361 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     9421 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
     9481 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagaccc
     9541 tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg
     9601 ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc
     9661 tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca
     9721 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     9781 aactggggca cagatgagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     9841 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9901 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9961 agcatcacaa atttcacaaa taaagcattt ttttcactgc caactttatt atacatagtt
    10021 gataattcac tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata
    10081 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
    10141 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
    10201 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
    10261 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga
    10321 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
    10381 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
    10441 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
    10501 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
    10561 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
    10621 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
    10681 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
    10741 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
    10801 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
    10861 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
    10921 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    10981 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11041 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11101 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11161 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    11821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12001 tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13081 ccgcgcacat ttccccgaaa agtg
//
