LOCUS       sequence-341984-        8648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved scaffold and puro/BFP
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     396..416
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             459..639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    686..811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1310..1543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1728..1772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2070..2187
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2262..2510
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2262..2282
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2433..2452
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2637..3136
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2672..2695)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3069..3088
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3176..3772
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3176..3195)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3672..3692
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3791..3844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3845..4546
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4606..4626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5024..5035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5487..5504)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5493..5717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5743..5763)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5743..5761)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5782..5798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5782..5798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5795..5817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5806..5822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5830..5836,5837..5854,5855..5860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5875..5896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6013..6030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6184..6772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6264..6283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6943..7734,7735..7803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7566..7585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7804..7908)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7934..8389)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8071..8092)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8283..8302
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8516..8538
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8530..8547
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8531..8547
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8557..8576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8557..8575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
       61 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      121 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      181 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      241 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      301 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      361 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      421 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1141 acagcaagcg gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt
     1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1921 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1981 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2041 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2101 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2161 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2221 tggttagtac cgggccctac gcgtccaagg tcgggcagga agagggccta tttcccatga
     2281 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     2341 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     2401 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     2461 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttcga
     2521 gaagacacgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     2581 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tggatccaat tctaccgggt
     2641 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2701 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     2761 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     2821 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     2881 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     2941 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     3001 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     3061 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     3121 atctccgggc ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac
     3181 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3241 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3301 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3361 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3421 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3481 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3541 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3601 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3661 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3721 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggcggcgg
     3781 gtccggagga gagggcagag gaagtctcct aacatgcggt gacgtggagg agaatcctgg
     3841 cccaatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     3901 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     3961 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     4021 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     4081 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaggacgg
     4141 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     4201 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     4261 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     4321 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     4381 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     4441 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     4501 cagatactgc gacctcccta gcaaactggg gcacaagctt aattgagcgg ccgctcaacc
     4561 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4621 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4681 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4741 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4801 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4861 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4921 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4981 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5041 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tacgccttcg
     5101 ccctcagacg agtcggatct ccctttgggc cgcctccccg caggtacctt taagaccaat
     5161 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5221 gctaattcac tcccaaagaa gtcaagatct gctttttgcc tgtactgggt ctctctggtt
     5281 agaccagagt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5341 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5401 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5461 tctagcagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5521 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5581 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5641 tggggtgggg caggacagca agggggagga ttgggaagtc aatagcaggc atgctgggga
     5701 tgcggtgggc tctatgggcg gccgttaatt aagcttttgt tccctttagt gagggttaat
     5761 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5821 aattccacac aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt
     5881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7921 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     7981 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8041 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8101 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8161 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8221 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8281 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     8341 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     8401 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8461 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8521 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     8581 ttgactagta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8641 ccattgac
//
