LOCUS       sequence-342040-        9433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. 
            Part of optogenetic system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
  TITLE     Mapping the dynamic transfer functions of eukaryotic gene 
            regulation.
  JOURNAL   Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003.
  PUBMED    34469745
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 
            10.1016/j.cels.2021.08.003."
COMMENT     Sequence Label: pJL15
FEATURES             Location/Qualifiers
     source          1..9433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..501
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        1928..2369
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     protein_bind    1928..2045
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    2253..2271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2274..2292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2340..2363
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             2396..2461
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2468..2488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2852..4687
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="KMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQF
                     YPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYD
                     PVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIE
                     SVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNE
                     FIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEE
                     SADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLV
                     DAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYI
                     SGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKAS
                     GVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEP
                     GLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVF
                     FVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     primer_bind     complement(5980..5999)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5981..5999)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6006..6023)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6006..6022)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6015..6037)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6163..6618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6250..6269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6460..6481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6974..6993)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7093..7115
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7153..7171)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7256..7360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7361..7429,7430..8221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7579..7598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8392..8980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8881..8900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9134..9151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9268..9289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9304..9309,9310..9327,9328..9334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9342..9358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9347..9369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9366..9382
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9366..9382
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9403..9421
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 cgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc
     1981 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
     2041 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
     2101 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
     2161 atgcgattag ttttttagcc ttatttctgg ggtactgcag cagcgaagcg atgatttttg
     2221 atctattaac agatatataa atgcaaaaac tgtccctatc agtgatagag atatccctat
     2281 cagtgataga gatattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta
     2341 atatacctct atactttaac gtcaaggaga aaaaaccctg ggccctaaaa aaatggacta
     2401 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
     2461 gatggccccc aagaaaaaga ggaaggtggg cattcacggg gtgccgggtg gaggttctgg
     2521 ttctagaccc ggggagcgcc ccttccagtg tcgcatttgc atgcggaact tttcgcgcca
     2581 ggacaggctt gacaggcata cccgtactca taccggtgaa aaaccgtttc agtgtcggat
     2641 ctgtatgcga aatttctccc agaaggagca cttggcgggg catctacgta cgcacaccgg
     2701 cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc agtcgccgcg acaacctgaa
     2761 ccggcaccta aaaacccacc tgagaggatc cggaggttct ggcggtggaa gtggtggcgg
     2821 aggtagcggt ggtggcagcg gttcctgcag gaagatggac aaaaagacta tagtttggtt
     2881 tagaagagac ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc
     2941 tgtttttcct gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc
     3001 ttcaagatgg tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg
     3061 atctgacctc actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg
     3121 cgttaccggt gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg
     3181 ggaccatacc gtaaaggaga agctggtgga acgtgggatc tctgtgcaaa gctacaatgg
     3241 agatctattg tatgaaccgt gggagatata ctgcgaaaag ggcaaacctt ttacgagttt
     3301 caattcttac tggaagaaat gcttagatat gtcgattgaa tccgttatgc ttcctcctcc
     3361 ttggcggttg atgccaataa ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga
     3421 actagggctg gagaatgagg ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc
     3481 tccaggatgg agcaatgctg ataagttact aaatgagttc atcgagaagc agttgataga
     3541 ttatgcaaag aacagcaaga aagttgttgg gaattctact tcactacttt ctccgtatct
     3601 ccatttcggg gaaataagcg tcagacacgt tttccagtgt gcccggatga aacaaattat
     3661 atgggcaaga gataagaaca gtgaaggaga agaaagtgca gatctttttc ttaggggaat
     3721 cggtttaaga gagtattctc ggtatatatg tttcaacttc ccgtttactc acgagcaatc
     3781 gttgttgagt catcttcggt ttttcccttg ggatgctgat gttgataagt tcaaggcctg
     3841 gagacaaggc aggaccggtt atccgttggt ggatgccgga atgagagagc tttgggctac
     3901 cggatggatg cataacagaa taagagtgat tgtttcaagc tttgctgtga agtttcttct
     3961 ccttccatgg aaatggggaa tgaagtattt ctgggataca cttttggatg ctgatttgga
     4021 atgtgacatc cttggctggc agtatatctc tgggagtatc cccgatggcc acgagcttga
     4081 tcgcttggac aatcccgcgt tacaaggcgc caaatatgac ccagaaggtg agtacataag
     4141 gcaatggctt cccgagcttg cgagattgcc aactgaatgg atccatcatc catgggacgc
     4201 tcctttaacc gtactcaaag cttctggtgt ggaactcgga acaaactatg cgaaacccat
     4261 tgtagacatc gacacagctc gtgagctact agctaaagct atttcaagaa cccgtgaagc
     4321 acagatcatg atcggagcag cacctgatga gattgtagca gatagcttcg aggccttagg
     4381 ggctaatacc attaaagaac ctggtctttg cccatctgtg tcttctaatg accaacaagt
     4441 accttcggct gttcgttaca acgggtcaaa gagagtgaaa cctgaggaag aagaagagag
     4501 agacatgaag aaatctaggg gattcgatga aagggagttg ttttcgactg ctgaatcttc
     4561 ttcttcttcg agtgtgtttt tcgtttcgca gtcttgctcg ttggcatcag aagggaagaa
     4621 tctggaaggt attcaagatt catctgatca gattactaca agtttgggaa aaaatggttg
     4681 caaatgagcg gccgccaccg cggtggagct ctaagcaaat agctaaatta tatacgaatt
     4741 aatattatga ttaagtgttt acgtgagtgc gatattttta ttactatctt atacagttgt
     4801 atatactcta taaaatgagt tgtctattaa ttaacgcgat gaatgctttc tgggtttacc
     4861 tctccaacaa ctctagttta cttctcaata cattcaattg tatttgattt gtcaatactt
     4921 catcattaat caattctata gttttgtttt tctcgtttat ttccaaattt aatgcatcaa
     4981 ttttattatt caatttgtcg ttgattttgg ttaatgattt tatggtttga tctctggcat
     5041 tgattgtttg tgttagtttt tcattattga taattaaatt atttaagtta gttatcaact
     5101 cggtgttttc aagtttcaag ttttcaattt ctttagagtt tattagattt gtcaaagttt
     5161 ctgaattgct tgattggtcc tgtagaagag tatttgttgt tgtggataat tgattcaatt
     5221 tttgagacaa ttgctggaag gcgttgaaat atctagcatc aatctcatgg tttttttccc
     5281 gagagtctcg tagattcaat tgttttaata tatcttggga ccactcttga tttgaactca
     5341 tggaaattaa actgggtgtt gtgttgtggt gtaatgattg tacccccttt gcttataatt
     5401 gtgtggtgac accgattatt taaagctgca gcatacgata tatatacatg tgtatatatg
     5461 tatacctatg aatgtcagta agtatgtata cgaacagtat gatactgaag atgacaaggt
     5521 aatgcatcat tctatacgtg tcattctgaa cgaggcgcgc tttccttttt tctttttgct
     5581 ttttcttttt ttttctcttg aactccagaa aaaaaatata aaagagatgg aggaacggga
     5641 aaaagttagt tgtggtgata ggtggcaagt ggtattccgt aagaacaaca agaaaagcat
     5701 ttcatattat ggctgaactg agcgaacaag tgcaaaattt aagcatcaac gacaacaacg
     5761 agaatggtta tgttcctcct cacttaagag gaaaaccaag aagtgccaga aataacagta
     5821 gcaactacaa taacaacaac ggcggctaca acggtggccg tggcggtggc agcttcttta
     5881 gcaacaaccg tcgtggtggt tacggcaacg gtggtttctt cggtggaaac aacggtggca
     5941 gcggccggcc atttaaatgt ttaaacggcc acgctggccc cctatagtga gtcgtattac
     6001 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6061 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6121 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     6181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6481 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6601 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6661 acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta
     6721 ttaggatctc tacccttggc gaaaagtcct ctgccaacaa tgatgatatc tgatccacca
     6781 cttacaactt cgtcgacggt tctgtactgc tgacccaata tgcgtatata taccaatcta
     6841 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     6901 caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aaggtggtat
     6961 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     7021 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7081 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7141 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7201 tttcttagac gtcggcgcgc cgaggacgtc aggtggcact tttcggggaa atgtgcgcgg
     7261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8521 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9181 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9241 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9301 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9361 tcacacagga aacagctatg accatgatta cgccaagctc ggaattaacc ctcactaaag
     9421 ggaacaaaag ctg
//
