LOCUS       sequence-342071-       15182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Rosa multiflora MYB108 for subcellular localization in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Dong J, Cao L, Zhang X, Zhang W, Yang T, Zhang J, Che D
  TITLE     An R2R3-MYB Transcription Factor RmMYB108 Responds to Chilling 
            Stress of Rosa multiflora and Conferred Cold Tolerance of 
            Arabidopsis.
  JOURNAL   Front Plant Sci. 2021 Jul 27;12:696919. doi: 
            10.3389/fpls.2021.696919. eCollection 2021.
  PUBMED    34386027
REFERENCE   2  (bases 1 to 15182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-07-27"; volume: "12:696919. doi"; pages: " 
            10.3389/fpls.2021.696919. eCollection 2021"
COMMENT     Sequence Label: pBI121-GFP-RmMYB108
FEATURES             Location/Qualifiers
     source          1..15182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    137..161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(1757..2905)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(3204..3998)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  4190..4957
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      5580..6291
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6418..6789)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6822..6931
                     /note="incP origin of transfer"
     primer_bind     7154..7173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7407..7424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(7612..8262)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    8727..8751
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8875..9054
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8907..9090
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9111..9905
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9165..9184)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9775..9794
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10295..10547
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    11096..11117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11132..11137,11138..11155,11156..11162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11170..11186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11175..11197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11194..11210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11194..11210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11734..12079
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     12022..12042
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     regulatory      13642..13651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13648..13650,13651..13653,13654..14367)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(13648..13650,13651..13653,13654..14364)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13693..13714)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13954..13973)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14301..14322
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      14424..14676
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(14685..14702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14685..14701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14694..14716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(14841..14860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15051..15072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
       61 actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
      121 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
      181 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
      241 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
      301 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
      361 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag
      421 atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct gtcgatcttg
      481 agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg
      541 ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc tcaccgaatg
      601 gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg ctgatcgacc
      661 ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc
      721 ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg
      781 agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg
      841 agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt
      901 tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct
      961 gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca
     1021 gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca
     1081 gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc
     1141 aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg
     1201 atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat
     1261 aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc
     1321 tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg
     1381 aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc
     1441 aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac
     1501 atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc
     1561 gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc
     1621 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
     1681 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
     1741 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
     1801 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
     1861 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
     1921 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
     1981 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
     2041 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
     2101 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
     2161 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
     2221 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
     2281 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
     2341 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
     2401 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
     2461 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
     2521 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
     2581 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
     2641 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
     2701 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
     2761 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
     2821 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
     2881 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
     2941 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
     3001 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
     3061 tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct
     3121 cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg
     3181 catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata atattttatt
     3241 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     3301 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     3361 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     3421 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     3481 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     3541 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     3601 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     3661 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     3721 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     3781 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     3841 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
     3901 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3961 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     4021 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
     4081 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
     4141 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc
     4201 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
     4261 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
     4321 gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca
     4381 ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat
     4441 gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa
     4501 actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc
     4561 ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta
     4621 cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc
     4681 gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga
     4741 tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata
     4801 tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg
     4861 gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct
     4921 gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga atttacaagc
     4981 tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat gctttgccca
     5041 ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat ccttacatac
     5101 ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc
     5161 aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatggcgctt
     5221 catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata atcttaccta
     5281 tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg cacccgcgac
     5341 cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc
     5401 cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac
     5461 ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct
     5521 cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc
     5581 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
     5641 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
     5701 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
     5761 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
     5821 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
     5881 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
     5941 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
     6001 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
     6061 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
     6121 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
     6181 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
     6241 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg ctcggtcttg
     6301 ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc
     6361 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     6421 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     6481 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     6541 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     6601 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     6661 cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     6721 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     6781 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     6841 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     6901 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     6961 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     7021 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgcc acgcttcccg
     7081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7441 agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga
     7501 aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg
     7561 tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc
     7621 accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac
     7681 aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat
     7741 cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc
     7801 atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat
     7861 cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc
     7921 gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc
     7981 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca
     8041 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag
     8101 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc
     8161 caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc
     8221 cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact
     8281 gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac
     8341 cagcggaggc tggtccggag gccagacgtg aaacccaaca tacccctgat cgtaattctg
     8401 agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc
     8461 ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg
     8521 tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg
     8581 cggcggccaa tcttgctcgt ctcgctggcc ggcgccagat ctggggaacc ctgtggttgg
     8641 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
     8701 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
     8761 agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac
     8821 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     8881 caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta agcacatacg
     8941 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt
     9001 tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct
     9061 catattcact ctcaatccaa ataatctgca ccggatctgg atcgtttcgc atgattgaac
     9121 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     9181 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     9241 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     9301 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     9361 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     9421 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     9481 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     9541 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     9601 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc
     9661 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     9721 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     9781 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9841 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     9901 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     9961 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    10021 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccacgggat
    10081 ctctgcggaa caggcggtcg aaggtgccga tatcattacg acagcaacgg ccgacaagca
    10141 caacgccacg atcctgagcg acaatatgat cgggcccggc gtccacatca acggcgtcgg
    10201 cggcgactgc ccaggcaaga ccgagatgca ccgcgatatc ttgctgcgtt cggatatttt
    10261 cgtggagttc ccgccacaga cccggatgat ccccgatcgt tcaaacattt ggcaataaag
    10321 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
    10381 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
    10441 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
    10501 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg cctcctgtca
    10561 atgctggcgg cggctctggt ggtggttctg gtggcggctc tgagggtggt ggctctgagg
    10621 gtggcggttc tgagggtggc ggctctgagg gaggcggttc cggtggtggc tctggttccg
    10681 gtgattttga ttatgaaaag atggcaaacg ctaataaggg ggctatgacc gaaaatgccg
    10741 atgaaaacgc gctacagtct gacgctaaag gcaaacttga ttctgtcgct actgattacg
    10801 gtgctgctat cgatggtttc attggtgacg tttccggcct tgctaatggt aatggtgcta
    10861 ctggtgattt tgctggctct aattcccaaa tggctcaagt cggtgacggt gataattcac
    10921 ctttaatgaa taatttccgt caatatttac cttccctccc tcaatcggtt gaatgtcgcc
    10981 cttttgtctt tggcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11101 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11161 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11221 ccaagcttgc atgcctgcag gtccccagat tagccttttc aatttcagaa agaatgctaa
    11281 cccacagatg gttagagagg cttacgcagc aggtctcatc aagacgatct acccgagcaa
    11341 taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
    11401 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc
    11461 agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga ttggagtctc
    11521 taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa tagaggacct
    11581 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
    11641 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
    11701 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
    11761 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
    11821 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
    11881 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
    11941 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
    12001 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
    12061 atttcatttg gagagaacac gggggactct agaatggatg tcaaaccagg aagggtttcg
    12121 gacatcatta gaaacagttc tgaggaagac gaagatgcta tgatgatgga cctgagaaga
    12181 gggccatgga cggttgagga agacgttgtt ctcatgaatt acattgctaa ccacggcgaa
    12241 ggtcgctgga actcccttgc tcgctgtgca ggtctgaaaa gaactggaaa gagctgcaga
    12301 ttaaggtggc tcaactatct ccgacccgac gttcgacgtg gcaacatcac tctcgaagaa
    12361 cagcttctca ttcttgagct tcattctcgc tggggcaacc gatggtccaa aattgcacag
    12421 cacttgcccg gaaggactga caatgaaatc aaaaactact ggaggactcg cgtgcaaaaa
    12481 cacgcaaagc agctcaagtg tgacgtcaac agcaagcagt tcaaggacac catgcgctac
    12541 ctctggatgc caagattggt cgagagaatt caagccgccg cgtccgccgc caccgccccc
    12601 accggagccc cttcttctat cacaaccgcc gcggccaaca cttatcattt caacaataac
    12661 aataacagct tccaagcccc cgcgggagca ggacacgtcg ctttacaaca gccaacgccc
    12721 attggcaata acaatgactt ggccggaagt tacaccactc cggaaaactc cagcactgcg
    12781 gcgtcatcgg actcgtttgg gactcaggtc tcgcctgtgt cagagctaac cgactattac
    12841 agtaatatgt cggttaacaa taataaacct agtagtcagg ctctggatta cttccaagcc
    12901 accaatcctc atcaccaagt cggttatcat gattccatga ctagtccatc tggttactca
    12961 ttcaatcaag gaggactaaa cagccataac tttcaagctg cttcggcgcc ggagcaaaac
    13021 aacaacggcc agtggagcat ggacggtggg gacttttcgg acaatctgtg gaacgttgag
    13081 gaatccgaca tgtggttctt acagcagcaa ctcggcaata tttctagagg atccatgtcg
    13141 tcggtgttca gcggagatga gaccgcgccc ttcttcggct tcctcggcgc cgccgccgcc
    13201 ctcgtcttct catgcatggg ggcggcctac gggacggcca agagcggcgt gggggtggcg
    13261 tccatgggcg tgatgcgccc ggagctcgtc atgaagtcca tcgtgcccgt cgtcatggcg
    13321 ggagtgctcg gaatctacgg cctcatcatc gccgtcatca tcagcaccgg gatcaacccc
    13381 aaggccaagc catacttcct cttcgacgga tacgcccacc tctcctcagg gctcgcatgt
    13441 ggcctcgcgg ggctcgccgc cggcatggcc atcggcaccg tcggtgacgc gggagtcagg
    13501 gcgaacgctc aacaaccaaa gctgttcgtg ggcatgatcc tcatcctcat tttcgctgaa
    13561 gcgctcgccc tttacggtct cattgtgggc atcatcctct cgtcccgcgc cggccaatcc
    13621 cgcgcagatt tggtaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
    13681 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
    13741 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
    13801 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
    13861 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
    13921 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
    13981 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
    14041 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
    14101 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
    14161 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
    14221 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
    14281 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
    14341 ctcggcatgg acgagctgta caagtaaagc ggccgctcga gcaagcttat gcatgcggcc
    14401 gcaattcgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
    14461 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
    14521 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
    14581 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
    14641 aattatcgcg cgcggtgtca tctatgttac tagatcggga attcactggc cgtcgtttta
    14701 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    14761 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    14821 cgcagcctga atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    14881 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    14941 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
    15001 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    15061 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
    15121 tttgccgatt tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
    15181 ga
//
