LOCUS       sequence-342073-        5884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mtrC from Shewanella oneidensis MR-1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   
  TITLE     Turn on the Mtr pathway genes under pLacI promoter in Shewanella 
            oneidensis MR-1
  JOURNAL   Bioresour. Bioprocess. 5, 35 (2018)
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour. 
            Bioprocess. 5, 35 (2018)"
COMMENT     Sequence Label: pETSXM2-pLacI-mtrC
FEATURES             Location/Qualifiers
     source          1..5884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1163..1183
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(1190..1217)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1233..1304)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             3306..3317
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    3335..3351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3359..3365,3366..3383,3384..3389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3404..3425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3526..3547
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3550..3593
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3597..3615)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(3843..4431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3923..3942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4542..4561)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4553..5368
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4553..5368
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4626..4645)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(join(5460..5884,1..31))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5597..5618)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5809..5828
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccgcgctta atgcgccgct acagggcgcg tcccattcgc caatccggat atagttcctc
       61 ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aagggatccg agcctttagg
      121 cgaactaggg cgaagccctt ggtcgcggcg cagccgccag atgctcaaat ttacttgttt
      181 atccacaggc gaaacccttt ttaatagatc tttcttgttt tttaagatct ttcttgtttt
      241 tagcgccgct gtagcccttg gtgctaaagg gctacagcgg tttaatgtgg agagatttac
      301 ggtaaaacag cccctaaagg tggagagatt tacggtaaaa caatccctaa aggtggagag
      361 atttacggtg atattcacag ttaattgtgg agaacgttga tagcaattcc acaattggtt
      421 attgtggaat ccccaaaaat aaactccaca atagtgacgt aaaatgaaca acctatctgt
      481 aactaaatca aatagcttga tagatgctag ttacaaacta aatgtacaag ctcaaaaact
      541 ggtcttagct tgcctcggga aagtagattc tagaggtaat gcaccaaagg aaatgaccct
      601 aacagccctt gaattctcag aacttatggg gatagacatc aaaaatgctc atagagagct
      661 atacaaggcg tctgataccc ttttcgatgc cgtaatactc ctaagagatg agcaagaaga
      721 ggtaaagctt aggtggattc aaaaaggagt gaaaaaactc aaaggacaag gagcaataac
      781 aatcacttgg actgatgaag ttttgaagta catcagtagt ttacagagca ggtttacaac
      841 ttataagctg cgacatattg cgaatctaca gtcagctcat tctattcgtt tatacgaact
      901 tttgatgaaa tttaacgcta ctggagaaag agttatctat gtggatgact tccgctcagc
      961 attaggtatc tccgacaaat acccacaatt tagagactta aataaatggg ttatcaagcc
     1021 ggccgtagat gaactaaatc agcgttcaga cctcaccata aattacgaga ccattaagaa
     1081 agggcgaaca gtggctgcat tgtcatttga gttcaagcag agcgcacagc taaaattaga
     1141 cgtataatca gtcgacccat ggctgcagcg gccgctacta gtatataaac gcagaaaggc
     1201 ccacccgaag gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata
     1261 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggctctagta
     1321 catatgttac attttcactt gagtgtgatc tgcaactgtt ggggtatggc agtagaaaca
     1381 ggtttctgac tgtgccgctt ggttggcttg agtataatca ccatcaataa cagcaccgta
     1441 gcttacaagg gtgtcgacag aatgtttggt cattttatct gtaccaatgg tatgacatga
     1501 ggtacaagtg gctgtgattg gggtggtata gccttttgtt gtcgctagtg cacctttcag
     1561 tttaaatgca tccaaattga agtcgttatg acattgggta cattctttaa tgataccttg
     1621 ctcaccatgc accacgtgta gtttcatctc taatgcaccg agtttagtcg ggctataggt
     1681 accgtctggt gtatggcatg ccacacaagc atcaacgccg acgataggct taccgcttgc
     1741 atctttggca tgtgaaactt gttcagacat tacaaagcct gcatggtggc ctttgtggat
     1801 ttcaaaggtg tcgccgtgac agttttggca tgtcgcaaac tcaaccgagt taacatggcg
     1861 catgcttggt gctttgccag acttagtgcc aaaggctaac tcggctttca tactggtggt
     1921 tgtgctatta gcggtacaag ctgttagatc tgtgccggta ttgcacattt ctagaccgat
     1981 gaagctaaag gcggtatcag tatcgcccgt gccaaatggc agggcaggtg tggtgaacac
     2041 cagtttaccg tcaactacgc tgacatctgc ttgcagtgcg ccatccttga ctaagtcttt
     2101 agtcactttc ttatattgtg ttactgggct tgggttatag cccatgatgg ggaagttagg
     2161 accaacgtta gtgatagttt ctaaacgttt gatcttattt tgtatgctcg ccgcatcaat
     2221 ggcattgccg tctttatcta agatagacac ggttaatacc gcagtattat cagcttgagc
     2281 agctaacgtt gcagacatac ctaattgagc gataacggct tttttgtcag cggttttagc
     2341 tgtatgtaac tcggctgtcc aatcactctt atgacaggcg atacagtttg agttatcgag
     2401 ctgttgtgag tggcctttac ccgcagcaaa atcgatgtct acgtgacagc tagaacagac
     2461 ttccattgtt ggaactcgtg accagttttt tgcctcggta agcatgtcgg attcaatgtg
     2521 acacacttgg caattatctt ggacaatttt ttgcgcaact tcagggactt tattgttttt
     2581 gccccaagct ttgttggcat tatgcacatt gtgaattaag tgactaaagg cgacatgggg
     2641 tttaccgcga ccatcagcaa actcttgtgt gtggcaagat atacaggttt cgacttcagt
     2701 tgcttggtga taaatctttt caccttcaac gtggcaagaa gcacatactt cgtggctaac
     2761 gatatttttg gtgtattgcg gagcattgcc ttggccgtcg aaatcttcaa ccatttcggc
     2821 aacggggacg gtcgttccgt ctgctaactt acccgcagca gaaacaacgt taaaccgttg
     2881 cgttaattgg gcattaaaaa ctttattgct atcgaaggcg tcgaatttaa aggtatagct
     2941 accgttttta ttatcgacat agctctttga agagcctaat ccttgccagt tagcactatt
     3001 accagggcct gtcgcccctt ccggtattaa ttgcagtgct tttttgattt ctaaatttgc
     3061 gagacccaca attggcatat cggcttcgtt ggtagcgaaa acagtgacca caggcgaacc
     3121 gttttcatag ctcactttcg tgatatcgag gtttacctta tggatagtac cagcgggttc
     3181 tccacctggc tcaccagggt taccatcggt accatcgcta ccaccacaac cagttaaggc
     3241 catggagatg gcacttgctg cgagcagcag tgcgattttt gatttttttg cgttctcatg
     3301 agctctttct cctctttctc tagtatgtgt gaaattgtta tccgctcaca attccacaca
     3361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgctcta gaagcggccg
     3541 cgaattccag aaatcatcct tagcgaaagc taaggatttt ttttatctga aattctgcct
     3601 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3661 tggcactcga gaatgcaaca cacgcaacac cgcatctctt gatgactagt gcgagcggta
     3721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagaag
     3901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4261 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4321 accttcggaa aaagagttgg tagctcttga tccggcaaac taaccaccgc tggtagcggt
     4381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4501 gtcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     4561 tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt
     4621 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     4681 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     4741 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     4801 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     4861 gaaaacagca ttccaggtat tagaagagta tcctgattca ggtgaaaata ttgttgatgc
     4921 gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc cttttaacag
     4981 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     5041 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     5101 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     5161 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     5221 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     5281 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     5341 tcatttgatg ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat
     5401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     5461 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5881 accc
//
