LOCUS       sequence-342097-       11041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: JAK2 (V617F)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..11041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..226
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        38..234
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      85..220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             266..868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(266..285)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     762..782
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    911..1028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1086..1586
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1505..1523
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    1607..1631
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5046..5070)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5078..5119
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5144..5732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5197..5217)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5615..5626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5804..6037
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6115..6236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6152..6171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6206..6225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6276..6411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6431..6450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6432..6450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6460..6477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6460..6476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6469..6491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6618..7073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6705..6724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6915..6936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7099..7203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7204..7272,7273..8064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7422..7441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8235..8823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8724..8743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8977..8994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9111..9132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9147..9152,9153..9170,9171..9177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9185..9201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9190..9212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9209..9225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9209..9225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9244..9264
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9246..9264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9292..9518
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9519..9699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9746..9871
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10364..10597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10782..10826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
       61 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      121 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
      241 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
      301 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
      361 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
      421 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
      481 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
      541 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
      601 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
      661 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
      721 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
      781 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
      841 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
      901 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      961 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1021 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     1081 gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1141 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1201 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1261 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1321 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1381 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     1441 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     1501 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     1561 accgaatcac cgacctctct ccccaggggc tagcatcgat ggatcaacaa gtttgtacaa
     1621 aaaagttggc tggtgcggcg gcggcaccat gggaatggcc tgccttacga tgacagaaat
     1681 ggagggaaca tccacctctt ctatatatca gaatggtgat atttctggaa atgccaattc
     1741 tatgaagcaa atagatccag ttcttcaggt gtatctttac cattcccttg ggaaatctga
     1801 ggcagattat ctgacctttc catctgggga gtatgttgca gaagaaatct gtattgctgc
     1861 ttctaaagct tgtggtatca cacctgtgta tcataatatg tttgctttaa tgagtgaaac
     1921 agaaaggatc tggtatccac ccaaccatgt cttccatata gatgagtcaa ccaggcataa
     1981 tgtactctac agaataagat tttactttcc tcgttggtat tgcagtggca gcaacagagc
     2041 ctatcggcat ggaatatctc gaggtgctga agctcctctt cttgatgact ttgtcatgtc
     2101 ttacctcttt gctcagtggc ggcatgattt tgtgcacgga tggataaaag tacctgtgac
     2161 tcatgaaaca caggaagaat gtcttgggat ggcagtgtta gatatgatga gaatagccaa
     2221 agaaaacgat caaaccccac tggccatcta taactctatc agctacaaga cattcttacc
     2281 aaaatgtatt cgagcaaaga tccaagacta tcatattttg acaaggaagc gaataaggta
     2341 cagatttcgc agatttattc agcaattcag ccaatgcaaa gccactgcca gaaacttgaa
     2401 acttaagtat cttataaatc tggaaactct gcagtctgcc ttctacacag agaaatttga
     2461 agtaaaagaa cctggaagtg gtccttcagg tgaggagatt tttgcaacca ttataataac
     2521 tggaaacggt ggaattcagt ggtcaagagg gaaacataaa gaaagtgaga cactgacaga
     2581 acaggattta cagttatatt gcgattttcc taatattatt gatgtcagta ttaagcaagc
     2641 aaaccaagag ggttcaaatg aaagccgagt tgtaactatc cataagcaag atggtaaaaa
     2701 tctggaaatt gaacttagct cattaaggga agctttgtct ttcgtgtcat taattgatgg
     2761 atattataga ttaactgcag atgcacatca ttacctctgt aaagaagtag cacctccagc
     2821 cgtgcttgaa aatatacaaa gcaactgtca tggcccaatt tcgatggatt ttgccattag
     2881 taaactgaag aaagcaggta atcagactgg actgtatgta cttcgatgca gtcctaagga
     2941 ctttaataaa tattttttga cttttgctgt cgagcgagaa aatgtcattg aatataaaca
     3001 ctgtttgatt acaaaaaatg agaatgaaga gtacaacctc agtgggacaa agaagaactt
     3061 cagcagtctt aaagatcttt tgaattgtta ccagatggaa actgttcgct cagacaatat
     3121 aattttccag tttactaaat gctgtccccc aaagccaaaa gataaatcaa accttctagt
     3181 cttcagaacg aatggtgttt ctgatgtacc aacctcacca acattacaga ggcctactca
     3241 tatgaaccaa atggtgtttc acaaaatcag aaatgaagat ttgatattta atgaaagcct
     3301 tggccaaggc acttttacaa agatttttaa aggcgtacga agagaagtag gagactacgg
     3361 tcaactgcat gaaacagaag ttcttttaaa agttctggat aaagcacaca gaaactattc
     3421 agagtctttc tttgaagcag caagtatgat gagcaagctt tctcacaagc atttggtttt
     3481 aaattatgga gtatgtttct gtggagacga gaatattctg gttcaggagt ttgtaaaatt
     3541 tggatcacta gatacatatc tgaaaaagaa taaaaattgt ataaatatat tatggaaact
     3601 tgaagttgct aaacagttgg catgggccat gcattttcta gaagaaaaca cccttattca
     3661 tgggaatgta tgtgccaaaa atattctgct tatcagagaa gaagacagga agacaggaaa
     3721 tcctcctttc atcaaactta gtgatcctgg cattagtatt acagttttgc caaaggacat
     3781 tcttcaggag agaataccat gggtaccacc tgaatgcatt gaaaatccta aaaatttaaa
     3841 tttggcaaca gacaaatgga gttttggtac cactttgtgg gaaatctgca gtggaggaga
     3901 taaacctcta agtgctctgg attctcaaag aaagctacaa ttttatgaag ataggcatca
     3961 gcttcctgca ccaaagtggg cagaattagc aaaccttata aataattgta tggattatga
     4021 accagatttc aggccttctt tcagagccat catacgagat cttaacagtt tgtttactcc
     4081 agattatgaa ctattaacag aaaatgacat gttaccaaat atgaggatag gtgccctagg
     4141 gttttctggt gcctttgaag accgggatcc tacacagttt gaagagagac atttgaaatt
     4201 tctacagcaa cttggcaagg gtaattttgg gagtgtggag atgtgccggt atgaccctct
     4261 acaggacaac actggggagg tggtcgctgt aaaaaagctt cagcatagta ctgaagagca
     4321 cctaagagac tttgaaaggg aaattgaaat cctgaaatcc ctacagcatg acaacattgt
     4381 aaagtacaag ggagtgtgct acagtgctgg tcggcgtaat ctaaaattaa ttatggaata
     4441 tttaccatat ggaagtttac gagactatct tcaaaaacat aaagaacgga tagatcacat
     4501 aaaacttctg cagtacacat ctcagatatg caagggtatg gagtatcttg gtacaaaaag
     4561 gtatatccac agggatctgg caacgagaaa tatattggtg gagaacgaga acagagttaa
     4621 aattggagat tttgggttaa ccaaagtctt gccacaagac aaagaatact ataaagtaaa
     4681 agaacctggt gaaagtccca tattctggta tgctccagaa tcactgacag agagcaagtt
     4741 ttctgtggcc tcagatgttt ggagctttgg agtggttctg tatgaacttt tcacatacat
     4801 tgagaagagt aaaagtccac cagcggaatt tatgcgtatg attggcaatg acaaacaagg
     4861 acagatgatc gtgttccatt tgatagaact tttgaagaat aatggaagat taccaagacc
     4921 agatggatgc ccagatgaga tctatatgat catgacagaa tgctggaaca ataatgtaaa
     4981 tcaacgcccc tcctttaggg atctagctct tcgagtggat caaataaggg ataacatggc
     5041 tggattgcca actttcttgt acaaagtggt taccggtggt aagcctatcc ctaaccctct
     5101 cctcggtctc gattctacgt agtaatgacc gcgttaagtc gacaatcaac ctctggatta
     5161 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5221 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5281 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5341 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5401 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5461 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5521 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     5581 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5641 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5701 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     5761 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5821 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5881 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5941 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6001 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     6061 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6241 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6421 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6481 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6541 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6601 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7081 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7141 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7201 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7261 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7321 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7381 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7441 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7501 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7561 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7621 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7681 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7741 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7801 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7861 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7921 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7981 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8041 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8101 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8341 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8941 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9001 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9061 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9181 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9241 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     9301 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     9361 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     9421 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     9481 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     9541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9721 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     9781 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9841 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9901 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9961 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10021 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    10081 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    10141 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    10201 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    10261 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10321 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10381 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    10441 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    10501 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    10561 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    10621 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    10681 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    10741 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    10801 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    10861 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    10921 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    10981 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
    11041 g
//
