LOCUS       sequence-342103-        3529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo homologous recombination substrate composed of two 
            differentially mutated GFP genes oriented as direct repeats and 
            separated by a drug selection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Pierce AJ, Johnson RD, Thompson LH, Jasin M
  TITLE     XRCC3 promotes homology-directed repair of DNA damage in mammalian 
            cells.
  JOURNAL   Genes Dev. 1999 Oct 15. 13(20):2633-8.
  PUBMED    10541549
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1999 Oct 15. 13(20):2633-8."
COMMENT     Sequence Label: pDRGFP
FEATURES             Location/Qualifiers
     source          1..3529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..51
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(316..336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     724..743
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    742..797
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     843..862
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     promoter        950..1447
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(985..1008)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1380..1399
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1466..2065
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1466..1485)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1962..1982
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2726..2847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2737..2756)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2791..2810
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3015..3036)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3364..3383
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
       61 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatccacgcc
      121 gggcttggcg aagtgcctca gctccaggtg cctcctcagg ttcctgctct gcctgatcac
      181 ggcgtagcag atggggcagg tggcgggctg ctcgctctgg ctcctgctcc tgggcttctc
      241 ggtgccgggg gggtgcttgg ccctcttggc cttgggggtg ctcagcacgc cgctggtgtt
      301 gcctcgtggg tcttctactt ttcgtttttt ttttggtggt gctgcatgct tcttcggcac
      361 ctttctcttc ttttttggcg atcttgatcc ctaaagcttg ggctgcaggt cgagggatct
      421 ccataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
      481 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
      541 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
      601 gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat
      661 atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc
      721 cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa
      781 aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata
      841 atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc
      901 agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattctaccg ggtaggggag
      961 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     1021 tacacaagtg gcctctggcc tcacacattc cacatccacc ggtaggcgcc aaccggctcc
     1081 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     1141 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     1201 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     1261 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     1321 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     1381 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     1441 cctttcgacc gatccagccg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     1501 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     1561 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     1621 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     1681 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     1741 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     1801 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     1861 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     1921 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     1981 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     2041 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     2101 gagcgcacga ccccatggct ccgaccgaag ccacccgggg cggccccgcc gaccccgcac
     2161 ccgcccccga ggcccaccgc gggggacaca ccgaacacgc cgaccctgct gaacacgcgg
     2221 cgcagttcgg tgcccaggag cggatcgaaa ttgatgatct attaaacaat aaagatgtcc
     2281 actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca gagtacctac
     2341 attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta gataaatgcc
     2401 tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag ttggatatca
     2461 taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc atgatctata
     2521 gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt gtggttctaa
     2581 gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca gcagcctctg
     2641 ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat cagaagcttc
     2701 agattaacgc ttacaattta cgccttaaga tacattgatg agtttggaca aaccacaact
     2761 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2821 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2881 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     2941 gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat gccgagagtg
     3001 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     3061 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     3121 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     3181 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     3241 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     3301 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     3361 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     3421 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     3481 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggca
//
