LOCUS       sequence-342131-       10164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a
            mNeonGreen fluorescent protein in its C-terminus. Inserted in a 
            lentiviral backbone. Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Bakhache W, Partiot E, Lucansky V, Bare Y, BonaventurB V, Goujon C, 
            Bories C, Deffieu M.S, Gaudin R
  TITLE     Pharmacological perturbation of intracellular dynamics as a 
            SARS-CoV-2 antiviral strategy
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro
FEATURES             Location/Qualifiers
     source          1..10164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4701..5408
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4953..4962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5412..5979
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5429..5981
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5573..5590)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5800..5819
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5993..6592
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5993..6012)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6489..6509
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6606..7194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6659..6679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7077..7088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7342..7522
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7569..7587)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7655..7759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7760..7828,7829..8620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7978..7997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8791..9379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9533..9550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9790..9811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9826..9831,9832..9849,9850..9856)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9864..9880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9869..9891
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9888..9904
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9888..9904
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9954..9973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc gccaccatga
     3421 gcgataacgg cccccaaaac caacggaatg cccctcggat aacatttggt ggccctagtg
     3481 attccacagg aagtaaccaa aacggagaac ggtctggagc ccgctctaaa cagaggcgac
     3541 cacagggatt gccaaataac acagcgtcat ggtttacagc cctgactcaa catggaaagg
     3601 aggacttgaa atttccacga ggacagggtg taccaataaa cacaaacagt agtccagacg
     3661 atcagatcgg gtattataga agggccacga gacgcatcag aggtggagat ggtaaaatga
     3721 aagatctcag tccgcgctgg tacttttatt atttgggaac cggcccagaa gcgggcctcc
     3781 catatggagc caataaggac gggattatat gggtggctac agagggagcc ctcaatacgc
     3841 caaaagatca catcggaaca agaaaccctg ctaataatgc cgcaatcgtg ctgcagttgc
     3901 ctcaggggac gacgcttcct aaaggctttt acgcagaagg atcacgcggc ggcagccaag
     3961 catcctcaag gtctagttcc agaagtcgaa acagctccag aaactccaca ccagggtcca
     4021 gtaggggcac aagtccggcg cggatggcgg gcaacggcgg agacgccgca ctcgctctgt
     4081 tgctcctgga ccggctcaac caacttgaat ccaagatgag tggtaaggga cagcagcaac
     4141 aaggtcaaac cgtaaccaag aaaagcgctg cagaagcttc taaaaaacct cgacaaaaac
     4201 ggaccgctac gaaggcatat aacgttactc aagccttcgg gagacgaggg ccggagcaaa
     4261 cccaggggaa ttttggagat caggagctca tccggcaagg gacagattac aaacattggc
     4321 ctcaaatcgc tcagttcgcg ccgtccgcaa gtgctttttt cggaatgtct cgcatcggta
     4381 tggaagtaac accatccggg acatggctca cttataccgg cgccatcaag ctggacgaca
     4441 aagatccaaa tttcaaagat caggtgattc tcttgaataa acacatagac gcgtataaga
     4501 cgtttccacc caccgaaccc aagaaggaca aaaagaaaaa agctgatgaa acacaagcgt
     4561 tgccccagcg acagaagaag cagcaaaccg tcaccctcct gccagccgct gatctcgatg
     4621 acttctccaa gcaacttcaa cagtccatga gctcagccga ttctactcag gcgggaggtt
     4681 ctggtggttc tggtggttcc gtgagcaagg gcgaggagga taacatggcc tctctcccag
     4741 cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac atggtgggtc
     4801 agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc accaagggtg
     4861 acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc catcagtacc
     4921 tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc tccggatacc
     4981 aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac taccgctaca
     5041 cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt ttccctgctg
     5101 acggtcctgt gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg aagaagactt
     5161 accccaacga caaaaccatc atcagtacct ttaagtggag ttacaccact ggaaatggca
     5221 agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg gcggctaact
     5281 atctgaagaa ccagccgatg tacgtgttcc gtaagacgga gctcaagcac tccaagaccg
     5341 agctcaactt caaggagtgg caaaaggcct ttaccgatgt gatgggcatg gacgagctgt
     5401 acaagtgagg atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5461 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5521 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5581 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5641 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5701 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5761 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5821 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5881 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5941 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga
     6001 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6061 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6121 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6181 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6241 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6301 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6361 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6421 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6481 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6541 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag
     6601 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6661 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6721 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6781 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6841 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6901 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6961 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7021 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7081 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7141 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     7201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7261 gggggactgg aagggctaat tcactcccaa cgaagacaag atcacctgca ggacaggcgc
     7321 gccctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7381 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7441 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7501 tcagtgtgga aaatctctag cacccgggcg attaaggaaa gggctagatc attcttgaag
     7561 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7621 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7981 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9541 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9601 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
     9661 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9721 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9781 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9841 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9901 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9961 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10021 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10081 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10141 aatatgtact acaaacttag tagt
//
