LOCUS       sequence-342166-        3795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The expression vector for genes of interest under mouse calmegin 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ikawa M, Nakanishi T, Yamada S, Wada I, Kominami K, Tanaka H, Nozaki
            M, Nishimune Y, Okabe M
  TITLE     Calmegin is required for fertilin alpha/beta heterodimerization and 
            sperm fertility.
  JOURNAL   Dev Biol. 2001 Dec 1;240(1):254-61. doi: 10.1006/dbio.2001.0462.
  PUBMED    11784061
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/dbio.2001";
            journalName: "Dev Biol"; date: "2001-12-1- 1"; volume: "240"; issue:
            "1"; pages: "254-61"
COMMENT     Sequence Label: pClgn1.1
FEATURES             Location/Qualifiers
     source          1..3795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     373..390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    507..528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(543..548,549..566,567..573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    581..597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     586..608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     605..621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     605..621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     640..660
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        642..660
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1147..1166)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1212..1267
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1266..1285)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1622..1641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1623..1641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1651..1668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1651..1667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1660..1682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1809..2264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1896..1915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2106..2127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2290..2394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2395..2463,2464..3255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2613..2632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3426..3795,1..219)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
       61 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      301 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      361 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      421 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      481 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      541 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      601 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      661 gaacaaaagc tggagctcat cgatgagctc cagagaggga ctgacgttgg aaggaccgca
      721 tccccccagg cgcgcggccg ctgggtcccg agcggcggtt gagcgggccg cttgcgcact
      781 ggggctgcca ctgcatcctg catcctccgg cgcggccgct cgcgcacgcg cagttagcag
      841 tcgctgctgg gcggctgccg gcgggatcgc tctggggaga cgccgggcac caagtagcgg
      901 ccagtcggac acctgagctg ccgctgctgg acacaagccc gcaggacagg tgtgtagacc
      961 gcgggccgcc ttcctgcggc ttgttctctg gggaccgggt gagaaatcgg atctctagag
     1021 gatccctgca ggaattcgat atcaagcttg tcgacgcggc cgcctcgaga ctcctcaggt
     1081 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     1141 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     1201 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     1261 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     1321 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     1381 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     1441 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     1501 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     1561 agtcatagct gtccctcttc tcttatgaag atccctcgac ttaattaagg tacccaattc
     1621 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     1681 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1741 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1801 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2041 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2221 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3781 acgacctaca ccgaa
//
