LOCUS       sequence-342167-        3875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Cartwright IM, Dowdell AS, Lanis JM, Brink KR, Mu A, Kostelecky RE, 
            Schaefer REM, Welch N, Onyiah JC, Hall CHT, Gerich ME, Tabor JJ, 
            Colgan SP
  TITLE     Mucosal acidosis elicits a unique molecular signature in epithelia 
            and intestinal tissue mediated by GPR31-induced CREB 
            phosphorylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2023871118. doi:
            10.1073/pnas.2023871118.
  PUBMED    33972436
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-18"; volume: "118(20). pii: 
            2023871118. doi"; pages: " 10.1073/pnas.2023871118"
COMMENT     Sequence Label: pKD279.8
FEATURES             Location/Qualifiers
     source          1..3875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      283..354
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      370..397
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(599..1187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(679..698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1275..1369)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1390..2049)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1964..1983
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2218..2236
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2257..2291
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      3052..3123
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3139..3166
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3400..3411
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3418..3875,1..256)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
       61 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
      121 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
      181 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
      241 ggatgaactg tacaaatgat gataataatc tagaccaggc atcaaataaa acgaaaggct
      301 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      361 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtaccc gtggatcctc
      421 tagagtcgac ctgcaggcat gcaagcttcc taggcgttcg gctgcggcga gcggtatcag
      481 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca tgcaagaaca
      541 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      781 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      901 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      961 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1021 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1081 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1141 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1201 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1261 tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1321 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1381 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1441 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1501 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1561 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     1621 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccat aacacgccac
     1681 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1741 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1801 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     1861 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1921 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1981 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     2041 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgaccgg
     2101 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgggccggc caacgtctca
     2161 ttttcgccag atatcgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2221 aggcgtatca cgaggccctt tcgtcttcac ctcgagctga cagctagctc agtcctaggt
     2281 ataatgctag ctgccccgta taataggtta taagacggaa ttatttatga aaattcttat
     2341 cgccgaagat gatattcata tccgccaagg gttggccgat atgctttcca gagagggata
     2401 ttccgttctg cttgctgata atggcaaagt tgccctgtta aaatatcaac aagagcagcc
     2461 ggattttatt attctggata ttatgatgcc tgagttagat ggctattcag tctgcaaaga
     2521 aatcagaaag cacgatgaac aaacgccggt gatcttttta tccgctaaag gtgaagagct
     2581 tgataaagtt ctggggcttg agcttggtgc ggatgactat attaataaac catttggtat
     2641 tcacgaggtt cgggcgcgaa tcaaaacaat tgcccgacgc tgtctgaaag ccaaacagaa
     2701 ttcaccagat caaagcgtgg agcttggcgg gctgacgatt tatcctgatc agaatgaggc
     2761 cgaatggaac agcgtccgga ttttgttttc ccaaaaagaa ttccagcttc tctcgatttt
     2821 tgtgagggag cataaaaaaa ttgtctctcg tgatgaacta ttggaggcgt tatgggatga
     2881 cgttgatttt gtcgatgaca atacgttaac ggtcaatgtc aatcggctgc ggagaaaact
     2941 ggagaatgca ggactgacag attgcatttc cacaatcaga ggacaaggtt atcaatttca
     3001 agtcaatcgg aaggatgaag cggaatgtta ataataatct agaccaggca tcaaataaaa
     3061 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3121 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tagtaaatca
     3181 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
     3241 atcataacgg ttctggcaaa tattctgaaa tgagctggtt agctagtcac accctcgtga
     3301 atgtgacagc attgtaagat tggggagcgg aattgcaaga aagttcgatg ggaggatgct
     3361 gacttccttt ttataataaa gaaaaaggag gagcagaaca aagaggagaa atactagatg
     3421 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
     3481 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
     3541 ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc gactctggta
     3601 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
     3661 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     3721 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     3781 cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg
     3841 gaatacaatt ttaacagcca caatgtttac atcac
//
