LOCUS       sequence-342168-        3768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SO_4387.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Cartwright IM, Dowdell AS, Lanis JM, Brink KR, Mu A, Kostelecky RE, 
            Schaefer REM, Welch N, Onyiah JC, Hall CHT, Gerich ME, Tabor JJ, 
            Colgan SP
  TITLE     Mucosal acidosis elicits a unique molecular signature in epithelia 
            and intestinal tissue mediated by GPR31-induced CREB 
            phosphorylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2023871118. doi:
            10.1073/pnas.2023871118.
  PUBMED    33972436
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-18"; volume: "118(20). pii: 
            2023871118. doi"; pages: " 10.1073/pnas.2023871118"
COMMENT     Sequence Label: pKD280.7
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..64
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        239..273
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     terminator      1828..1899
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1915..1942
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2142..2933
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      2945..3039
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3153..3698
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3599..3618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
       61 cgaggaagcg gaatatatcc cctaggcgtt cggctgcggc gagcggtatc agctcactca
      121 aaggcggtaa tacgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg
      181 gataatgttt tttgcgccga catcatagcg gttctggcaa atattctgaa atgagctgtt
      241 tacggctagc tcagccctag gtattatgct agcaaaaatt aagttctata ttacagctta
      301 tagtagaatg acacagaatt atgaacacta cctttttcta tttgttatca tcactggcct
      361 gataatggga gttattagtt attttggctt tcgagcgctg acacaagaaa gcaccctgaa
      421 tgaataccaa actcaacaat tggctaaaag ccacgtagct caaactcaat catttattct
      481 gcaacagctg aatcaaaaac aggttagatt agatgccatg ctagcctatc tgtctctaga
      541 taaaaatgcg ctacatacct tgatatccca agacagcgat atcgagaaca tctttgttct
      601 gcaaaaaaat aagctcgctt ttcctgatat atctcaacct ttaagtttga aagatcaaac
      661 gctggctgaa ctgattatgc caataagcca tgaccctaca ttgttaatgg ctaaacaacc
      721 cacatctgaa gataaaagcc ctaactctgg ctggttagtc ttacaagaca ataactatcc
      781 agcacttatt tactggcgta aaaccgatga agatacggta ggtataaaac tctcttacgt
      841 taaattttta gctgatgtta tctctgctct cgatacaaat tttacacctg atggtataaa
      901 aatttccgat aacggccagg tactttacca actgtcccca gagcctatcg gtaataatca
      961 aacgccttct tatagccaga atttaccttt tccgctacac ggttggcaga ttgactatta
     1021 taccgcacct gtgaaagggc aatctcttta ctatctaggc accatattaa ttgtcaccat
     1081 tattttggcc gttggcttta tggcatttat gctatatcgc gagtttaatc gcgcaaccag
     1141 attagcaagc caacaggtta gttttgtcgg acaggtttct cacgaactta aaacgccact
     1201 gacgaatatc acgctttatg ctgaaatgct taacgaaatg gaagcagagg aaaacgacca
     1261 gagagcccat tatcttggag tcattatcag tgaaagccaa cgcctatctc gcctgattca
     1321 aaacgtgttg accttcacca aactgccaca aatttgcatt cgccaagtcg atattaatca
     1381 attaatgaat caaatttata ctgcgttcaa acccgtattt gaagccaaag aaatccatct
     1441 caacctcaat attaatgggc aattatcaac gaaaacagac gttgatctgg taacccagat
     1501 tatcagtaac ctactcagta atgccgaaaa atatgctgca acaggtaagc aggttgacct
     1561 taatgtcgaa caagataacg acaatatttt tatacatatc cgagactacg gtaacggcat
     1621 aacggctaaa gagttaaaac aaatattcca gccgttttat cgggttagat catcgattac
     1681 cgaaggagtg agcggaacag gtatcggact caccattgcc cgccagttag ctgaaacgct
     1741 gagcggtgag attaacgcaa tcaacaatga gcccggaatg acgtttacgc taagacttcc
     1801 aaaacgctaa taatctagac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1861 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1921 ccttcgggtg ggcctttctg cgtttatagg tacccgggga tcctctagag tcgacctgca
     1981 ggcatgcaag cttagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     2041 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     2101 gatcaagatc tgatcaagag acaggatgag gatcgtttcg tatgagggaa gcggtgatcg
     2161 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2221 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2281 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2341 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2401 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2461 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2521 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2581 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2641 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2701 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2761 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2821 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2881 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taagagctcg
     2941 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     3001 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcactag taacaactta
     3061 tatcgtatgg ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag
     3121 taatatttta tctgattaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc
     3181 tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct
     3241 accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt
     3301 tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt
     3361 ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc
     3421 ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     3481 aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg
     3541 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg
     3601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     3661 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct
     3721 cacttccctg ttaagtatct tcctggcatc ttccgggaaa tctccgcc
//
