LOCUS       sequence-342172-        1070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for a UDP-GlcNAc sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Li Z, Zhang J, Ai HW
  TITLE     Genetically Encoded Green Fluorescent Biosensors for Monitoring 
            UDP-GlcNAc in Live Cells.
  JOURNAL   ACS Cent Sci. 2021 Oct 27;7(10):1763-1770. doi: 
            10.1021/acscentsci.1c00745. Epub 2021 Sep 30.
  PUBMED    34729420
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.1c00745"; journalName: "ACS Cent Sci"; date: 
            "2021-10-27- 27"; volume: "7"; issue: "10"; pages: "1763-1770"
COMMENT     Sequence Label: pcDNA3-UGAcS
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ganntataga atagggccct taatgatggt ggtgatggtg cgcccgggca acccggctca
       61 cttcatttgc cactcgctcg gtggcatccg gaatggatgc agcacgggcg cgttctgcca
      121 tggttaataa ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa
      181 gctgtggctg ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt
      241 attgctggcg gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga
      301 tttcactcgc cgttaacgca ccggagcggc aaacgacgac atccgcccac gcatacgccg
      361 ccgccatatc atcaataaat cccgtcacct tatgctgtgg ttgccctgct tcggcatacg
      421 cctgttcaac ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac
      481 ccagtttcgc agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc gtcgtaccac
      541 ccactaccag cacacgaacc ggaccttcac gtccagccaa acgttgccgc ggcaacggca
      601 gcgccaacac atcggtacgc accgggttac ccactacttc cgcattaggg aaagcacctg
      661 gaaacgcctg catcactttg gtggcaatct tcgccagcca tttattggtt aagcccgcaa
      721 taccgttttg ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccacctg
      781 gacctgacac gtagcctccc ataccgagca ccacgtcagg tttgtacgct ttcataatcg
      841 cccgcgcctg ctcccaggcg ttgaagatac gcagcggggc anctatcagt gcttttatac
      901 cttntccacg cagagnctcg tactcnagct tgtgccccag gangntgccg tcctccttga
      961 agtcaatgcc cttnngctcg atgnnggtca cagggtgnng cccntcaact cncctcgnng
     1021 cgggntctng nanntgcngt cntcntnnaa nnnangntgc acncctngaa
//
