LOCUS       sequence-342180-        5239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   O'Reilly AM, Pluskey S, Shoelson SE, Neel BG
  TITLE     Activated mutants of SHP-2 preferentially induce elongation of 
            Xenopus animal caps.
  JOURNAL   Mol Cell Biol 2000 Jan;20(1):299-311.
  PUBMED    10594032
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2000-01"; volume: "20(1)"; pages: "299-311"
COMMENT     Sequence Label: pSP64R1 SHP2 WT
FEATURES             Location/Qualifiers
     source          1..5239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           12..54
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           2070..2195
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(2307..2323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2307..2323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2320..2342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2331..2347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2355..2361,2362..2379,2380..2385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2400..2421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2538..2555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2709..3297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2789..2808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3468..4259,4260..4328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4091..4110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4329..4433)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4501..4519
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4557..4579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4679..4698
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4912..4931
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5221..5239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5221..5238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacaagctt gcttgttctt tttgcagaag ctcagaataa acgctcaact ttggcagatc
       61 ggtaccgaat tccgggcggg aggaacatga catcgcggag atggtttcac ccaaatatca
      121 ctggtgtgga ggcagaaaac ctactgttga caagaggagt tgatggcagt tttttggcaa
      181 ggcctagtaa aagtaaccct ggagacttca cactttccgt tagaagaaat ggagctgtca
      241 cccacatcaa gattcagaac actggtgatt actatgacct gtatggaggg gagaaatttg
      301 ccactttggc tgagttggtc cagtattaca tggaacatca cgggcaatta aaagagaaga
      361 atggagatgt cattgagctt aaatatcctc tgaactgtgc agatcctacc tctgaaaggt
      421 ggtttcatgg acatctctct gggaaagaag cagagaaatt attaactgaa aaaggaaaac
      481 atggtagttt tcttgtacga gagagccaga gccaccctgg agattttgtt ctttctgtgc
      541 gcactggtga tgacaaaggg gagagcaatg acggcaagtc taaagtgacc catgttatga
      601 ttcgctgtca ggaactgaaa tacgacgttg gtggaggaga acggtttgat tctttgacag
      661 atcttgtgga acattataag aagaatccta tggtggaaac attgggtaca gtactacaac
      721 tcaagcagcc ccttaacacg actcgtataa atgctgctga aatagaaagc agagttcgag
      781 aactaagcaa attagctgag accacagata aagtcaaaca aggcttttgg gaagaatttg
      841 agacactaca acaacaggag tgcaaacttc tctacagccg aaaagagggt caaaggcaag
      901 aaaacaaaaa caaaaataga tataaaaaca tcctgccctt tgatcatacc agggttgtcc
      961 tacacgatgg tgatcccaat gagcctgttt cagattacat caatgcaaat atcatcatgc
     1021 ctgaatttga aaccaagtgc aacaattcaa agcccaaaaa gagttacatt gccacacaag
     1081 gctgcctgca aaacacggtg aatgactttt ggcggatggt gttccaagaa aactcccgag
     1141 tgattgtcat gacaacgaaa gaagtggaga gaggaaagag taaatgtgtc aaatactggc
     1201 ctgatgagta tgctctaaaa gaatatggcg tcatgcgtgt taggaacgtc aaagaaagcg
     1261 ccgctcatga ctatacgcta agagaactta aactttcaaa ggttggacaa gggaatacgg
     1321 agagaacggt ctggcaatac cactttcgga cctggccgga ccacggcgtg cccagcgacc
     1381 ctgggggcgt gctggacttc ctggaggagg tgcaccataa gcaggagagc atcatggatg
     1441 cagggccggt cgtggtgcac tgcagtgctg gaattggccg gacagggacg ttcattgtga
     1501 ttgatattct tattgacatc atcagagaga aaggtgttga ctgcgatatt gacgttccca
     1561 aaaccatcca gatggtgcgg tctcagaggt cagggatggt ccagacagaa gcacagtacc
     1621 gatttatcta tatggcggtc cagcattata ttgaaacact acagcgcagg attgaagaag
     1681 agcagaaaag caagaggaaa gggcacgaat atacaaatat taagtattct ctagcggacc
     1741 agacgagtgg agatcagagc cctctcccgc cttgtactcc aacgccaccc tgtgcagaaa
     1801 tgagagaaga cagtgctaga gtctatgaaa acgtgggcct gatgcaacag cagaaaagtt
     1861 tcagatgaga aaacctgcca aaacttcagc acagaaatag atgtggactt tcaccctctc
     1921 cctaaaaaga tcaagaacag acgcaagaaa gtttatgtga agacagaatt tggatttgga
     1981 aggcttgcaa tgtggttgac taccttttga taagcaaaat ttgaaaccat ttaaagaccg
     2041 gaattcctcg agagatctgg ttaccactaa accagcctca agaacacccg aatggagtct
     2101 ctaagctaca taataccaac ttacacttta caaaatgttg tcccccaaaa tgtagccatt
     2161 cgtatctgct cctaataaaa agaaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa
     2221 aaaaaaaaaa aaaaamcccc cccccccccc ccctgcaggt cgactctaga ggatccccgg
     2281 gcgagctcga attaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     2341 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2401 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2461 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2521 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2581 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2641 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2701 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2761 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2821 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2881 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2941 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3001 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3061 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3121 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4441 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4501 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4561 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4621 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     4681 gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg
     4741 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     4801 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     4861 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     4921 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     4981 ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg
     5041 cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt
     5101 ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta
     5161 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     5221 atttaggtga cactataga
//
