LOCUS       sequence-342186-        4521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cry2PHR with C-terminal IBAR  and WH2 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wurz AI, Bunner WP, Szatmari EM, Hughes RM
  TITLE     CRY-BARs: Versatile light-gated molecular tools for the remodeling 
            of membrane architectures.
  JOURNAL   J Biol Chem. 2022 Aug 18;298(10):102388. doi: 
            10.1016/j.jbc.2022.102388.
  PUBMED    35987384
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-08-18"; volume: "298(10):102388. doi"; pages: " 
            10.1016/j.jbc.2022.102388"
COMMENT     Sequence Label: Cry2PHR-IBAR-WH2
FEATURES             Location/Qualifiers
     source          1..4521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1494
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      1513..1522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1519..2226
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1664..1682)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1932..1951
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat
       61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct
      121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt
      181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac
      241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt
      301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg
      361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata
      421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat
      481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg
      541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa
      601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta
      661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt
      721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac
      781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga
      841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata
      901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct
      961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg
     1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg
     1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat
     1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc
     1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc
     1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg
     1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt
     1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta
     1441 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcagcccgg
     1501 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1561 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1621 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1681 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1741 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1801 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1861 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1921 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1981 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2041 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2101 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2161 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     2221 tacaagggcg gcagcggcgg cagcatggag gctgtgattg agaaggaatg cagcgcgctc
     2281 ggaggcctct tccagaccat catcagcgac atgaagggga gctatccagt ttgggaagat
     2341 ttcataaaca aagcaggaaa gctgcagtcc cagcttcgga caacagtagt agcagcagct
     2401 gccttcttgg acgcctttca gaaagtggct gacatggcca ccaacacacg tggtgggacc
     2461 agggagattg gatctgctct caccaggatg tgcatgaggc acagaagcat tgaagccaag
     2521 ctgaggcagt tttcgagcgc tttaattgat tgtctgataa acccacttca agaacagatg
     2581 gaagaatgga agaaagtggc caaccagctg gataaagacc acgcaaaaga atataagaaa
     2641 gcccgccaag agataaaaaa gaagtcctcg gatacgctga aactgcagaa gaaagcaaaa
     2701 aaagtggaca ctcttgggag aggtgatatc cagcctcagt tggacagtgc tctccaagat
     2761 gtcaatgata agtatctctt attggaagaa acagaaaagc aggctgtccg gaaggctttg
     2821 attgaagaac gtggccgatt ctgtaccttc atctctatgc tgcggccagt gattgaagaa
     2881 gaaatctcaa tgctagggga aataacccac cttcagacca tctcggaaga tctaaaaagc
     2941 ctgaccatgg accctcacaa actgccctcc tcaagtgaac aggtgattct ggacttgaaa
     3001 ggttctgatt acagctggtc gtatcagacg ccaccctctt cccccagcac caccatgtcc
     3061 agaaagtcca gtgtctgcag cagcctgaac agtgtcaaca gcagtgactc ccggtccagc
     3121 ggctcccact cgcattcccc cagctcacat taccgctacc gcagctccaa cctggcccag
     3181 caggctcctg tgaggctgtc cagcgtgtcc tcccatgact caggattcat atcccaggat
     3241 gccttccagt ccaagtcacc atcccccatg ccgccagagg cccccaacca gttgtctaac
     3301 gggttttctc actatagttt atcaagtgag tcccacgtgg ggcccacggg tgcaggcctt
     3361 ttccctcatt gcctgcctgc ctctcgcctg ctccctcggg tcacctctgt ccaccttcca
     3421 gactacgctc attattacac cattgggccc ggcatgttcc cgtcatctca gatccctagc
     3481 tggaaggact gggctaagcc tgggccctat gaccagcctc tggtgaacac cctgcagcgc
     3541 cgcaaagaga agcgagaacc ggaccccaac gggggaggac ccactaccgc cagcggccca
     3601 cccgcagcag ctgaggaggc tcagaggcca cggagcatga ctgtatcggc tgccaccagg
     3661 cctggtgagg agatggaggc ttgtgaggag ctggccctgg ccctgtctcg gggcctgcag
     3721 ctggacaccc agaggagcag ccgggactcg cttcagtgct ccagcggcta cagcacccag
     3781 acaaccaccc cctgctgctc tgaggacacc atcccttccc aagtttcaga ttatgattat
     3841 ttctctgtaa gtggtgacca ggaggcagat cagcaggagt tcgacaagtc ctccaccatt
     3901 ccaagaaaca gcgacatcag ccagtcctac cgacggatgt tccaagccaa gcgtccagcc
     3961 tcaactgctg gcctccccac caccctggga cctgctatgg tcactccagg ggttgcaact
     4021 atccgacgga ccccttccac caagccttct gtccgccggg gaaccattgg agctggtccc
     4081 atccccatca agacacccgt gatccctgtc aagaccccaa ccgtcccaga cctcccaggg
     4141 gtgttgccag cccctccaga tgggccagaa gagcgcgggg agcacagccc tgagtcgcca
     4201 tctgtgggtg agggccccca aggtgtcacc agcatgccct cctcaatgtg gagcggccaa
     4261 gcttccgtta accctccact tccaggcccg aagcccagta tccctgagga gcacagacag
     4321 gcaattccag aaagtgaagc tgaagaccag gaacgggaac ccccaagtgc cactgtctcc
     4381 ccaggccaga ttccagagag tgaccctgca gacctgagcc caagggatac tccacaagga
     4441 gaagacatgc tgaacgccat ccgaaggggc gtgaaactga agaagaccac gacaaacgat
     4501 cgctcagccc ctcgcttttc t
//
