LOCUS       sequence-342199-        5404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent par-6(delPB1) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK259
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..42
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(57..62,63..80,81..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     100..122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     119..135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     119..135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     154..174
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        156..174
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2711..2727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2752..2771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2753..2771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2781..2798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2781..2797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2790..2812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2939..3394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3026..3045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3236..3257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3420..3524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3525..3593,3594..4385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3743..3762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4556..5144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5045..5064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5298..5315
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
       61 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      121 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
      181 aagctggagc tccaccgcgg tggcggccgc tctagaaggg ctgcaggaat tcgattggat
      241 ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag
      301 ccaaattcgt tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa
      361 tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa
      421 ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg
      481 ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa
      541 cgtttatttt gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa
      601 tatggtctcc gagctcatta aagaaaacat gcatatgaag ctctacatgg agggaaccgt
      661 caacaaccac cacttcaagt gcacctccga gggagaggga aagccatacg agggaaccca
      721 aaccatgcgt atcaaggccg tcgagggagg accactccca ttcgccttcg acatcctcgc
      781 cacctccttc atgtacggat ccaaggtaag tttaaacata tatatactaa ctaaccctga
      841 ttatttaaat tttcagacct tcatcaacca cacccaagga atcccagact tcttcaagca
      901 atccttccca gagggattca cctgggagcg tgtcaccacc tacgaggacg gaggagtcct
      961 caccgccacc caagacacct ccctccaaga cggatgcctc atctacaacg tcaaggtaag
     1021 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagatcc gtggagtcaa
     1081 cttcccatcc aacggaccag tcatgcaaaa gaagaccctc ggatgggagg cctccaccga
     1141 gaccctctac ccagccgacg gaggacttga gggacgtgcc gacatggccc tcaagctcgt
     1201 cggaggagga cacctcatct gcaacctcaa ggtaagttta aacatgattt tactaactaa
     1261 ctaatctgat ttaaattttc agaccaccta ccgttccaag aagccagcca agaacctcaa
     1321 gatgccagga gtctactacg tcgaccgtcg tcttgagcgt atcaaggagg ccgacaagga
     1381 gacctacgtc gagcaacacg aggtcgccgt cgcccgttac tgcgacctcc catccaagct
     1441 cggacaccgt aagatgtcct acaacggctc ctaccatcaa aatcatcatt caacatcaat
     1501 accaatgcat tctgcgtctg gtgtctctta cgatgggttc cggagccttg tcgagaagct
     1561 tcatcacctg gaaagtgtcc aattcacact gtgctacaac tcgactggcg gtgatcttct
     1621 accaatcaca aatgacgata acctccgaaa atcgttcgaa tcggctcgcc cactgctccg
     1681 tttgctcatt caacgccgcg gtgaatcatg ggaagagaaa tatggttatg gtacagactc
     1741 tgataaacga tggaagggaa tttcatcact tatggcacaa aaaccaccga aacgcagtta
     1801 ttcaatctcg aatcccgaag attttcgaca ggtctctgca atcatcgacg tggacattgt
     1861 gccagaagct catcgccgtg tccgtctctg caagcacgga caagaacgac cacttggatt
     1921 ctatattcgt gacggaacat cggttcgagt gacagaacga ggcgtcgtca aagtttcagg
     1981 aattttcatt tcccggcttg tcgatggtgg gctcgccgag tccacaggcc ttcttggtgt
     2041 taatgacgag gtgctcgagg tgaatggaat tgaggttctc ggaaagacgc tggatcaggt
     2101 cactgatatg atggtcgcca atgctcataa cttgattatt actgtaaaac ctgccaacca
     2161 acgaaacaca ctgtcacgag gaccgtcaca acaaggaaca ccgaacgcca gtgagatgtc
     2221 ggccgccacc gcagccgcta caggtggaat tcagcgcccg atgaaaatga acggaagctc
     2281 cgacggcagt tatcatccga aacagcacga cgcaaatgat tcggacagtg gagaggacac
     2341 gtaatccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     2401 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     2461 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     2521 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     2581 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     2641 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttagtaa
     2701 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
     2761 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2941 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3001 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3061 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3121 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3181 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3241 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3301 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3361 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     3421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggcccaatac gcaaaccgcc
     5341 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5401 agcg
//
