LOCUS       sequence-342210-        5446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent par-6(WT) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK258
FEATURES             Location/Qualifiers
     source          1..5446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    37..58
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(73..78,79..96,97..103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     116..138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     135..151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     135..151
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     170..190
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        172..190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2769..2785)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2770..2786)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2810..2829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2811..2829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2839..2856)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2839..2855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2848..2870)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2997..3452
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3084..3103)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3294..3315
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3478..3582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3583..3651,3652..4443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3801..3820)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4614..5202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5103..5122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5356..5373
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
       61 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      181 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaagggctgc
      241 aggaattcga ttggattttt gctttcgtcg taaatctaca cacgcgtctc ttccgtgcga
      301 gagtccaagc cagcagccaa attcgttgac tgagtattca acgtttatac gttgtcggca
      361 acgagaaata ggaaaatgca tcgggaaatg ttcttttttc gattttttcc aaggttttga
      421 caaattttac cacgaatttt gctatgtttt caattaaaaa atatgttatt caactgtttc
      481 tatgaggaaa ataaggcttt gcatgtaatt ttcttattca gcataatttt taattaattt
      541 gaattttctg tcctaacgtt tattttgttt tcttggttat gactgatctg aaattaattt
      601 ttgaatttta aggtaatatg gtctccgagc tcattaaaga aaacatgcat atgaagctct
      661 acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga gagggaaagc
      721 catacgaggg aacccaaacc atgcgtatca aggccgtcga gggaggacca ctcccattcg
      781 ccttcgacat cctcgccacc tccttcatgt acggatccaa ggtaagttta aacatatata
      841 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc
      901 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg
      961 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct
     1021 acaacgtcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1081 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat
     1141 gggaggcctc caccgagacc ctctacccag ccgacggagg acttgaggga cgtgccgaca
     1201 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cctcaaggta agtttaaaca
     1261 tgattttact aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc
     1321 cagccaagaa cctcaagatg ccaggagtct actacgtcga ccgtcgtctt gagcgtatca
     1381 aggaggccga caaggagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg
     1441 acctcccatc caagctcgga caccgtaaga tgtcctacaa cggctcctac catcaaaatc
     1501 atcattcaac acttcaagtc aaatcgaaat ttgattctga atggcgtcgt ttctcaatac
     1561 caatgcattc tgcgtctggt gtctcttacg atgggttccg gagccttgtc gagaagcttc
     1621 atcacctgga aagtgtccaa ttcacactgt gctacaactc gactggcggt gatcttctac
     1681 caatcacaaa tgacgataac ctccgaaaat cgttcgaatc ggctcgccca ctgctccgtt
     1741 tgctcattca acgccgcggt gaatcatggg aagagaaata tggttatggt acagactctg
     1801 ataaacgatg gaagggaatt tcatcactta tggcacaaaa accaccgaaa cgcagttatt
     1861 caatctcgaa tcccgaagat tttcgacagg tctctgcaat catcgacgtg gacattgtgc
     1921 cagaagctca tcgccgtgtc cgtctctgca agcacggaca agaacgacca cttggattct
     1981 atattcgtga cggaacatcg gttcgagtga cagaacgagg cgtcgtcaaa gtttcaggaa
     2041 ttttcatttc ccggcttgtc gatggtgggc tcgccgagtc cacaggcctt cttggtgtta
     2101 atgacgaggt gctcgaggtg aatggaattg aggttctcgg aaagacgctg gatcaggtca
     2161 ctgatatgat ggtcgccaat gctcataact tgattattac tgtaaaacct gccaaccaac
     2221 gaaacacact gtcacgagga ccgtcacaac aaggaacacc gaacgccagt gagatgtcgg
     2281 ccgccaccgc agccgctaca ggtggaattc agcgcccgat gaaaatgaac ggaagctccg
     2341 acggcagtta tcatccgaaa cagcacgacg caaatgattc ggacagtgga gaggacacgt
     2401 aatccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     2461 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     2521 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     2581 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     2641 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     2701 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttagtaatc
     2761 aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
     2821 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2881 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2941 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     3001 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3061 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3121 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3181 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3241 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3301 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3361 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3421 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     3481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4741 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5221 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5281 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5341 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg cccaatacgc aaaccgcctc
     5401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtt
//
