LOCUS       sequence-34222-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1612-HQ858849
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      347..356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatgga gcttgacgag gagtccttct tggatgagct catgtcgctg
      181 cggcgtgatg gatcggcgcc gtggcaggcg ccgccgtacc cgggtggcgg cggcggcggc
      241 ggcggtggcg gcatgatgat gagtgacctc ctcttctacg gcggcgacgg cggcagcgcg
      301 gaggcgaggg gcgggatgga cgcgtcgccg ttccaggagc tggcgtccat ggcggcgccg
      361 ccgcctcagc atccgcacga ggagttcaac ttcgactgct tgagcgaggt ctgcaacccg
      421 taccgcagct gcggcgccca gctcgtcccc tcggaggcgg ctagccagac gcagacgcag
      481 ctcactcctc tccgcgacgc aatggtggcg gaggaggaga cgagcggcga caaggcgctg
      541 ctccatggcg gcggcggctc gtcgtcgccg acgttcatgt ttggaggtgg cgccggagag
      601 agctcggaga tgatggccgg catcagaggc gtcggcggcg gcgtccaccc gcggagcaag
      661 ctccacggca cgccgtcgaa gaacctcatg gccgagaggc ggcgtcgcaa gcggctcaac
      721 gaccggctgt ccatgctccg gtc
//
