LOCUS       sequence-342232-        7131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CopyCatcher insertion donor for the white locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Li Z, Marcel N, Devkota S, Auradkar A, Hedrick SM, Gantz VM, Bier E
  TITLE     CopyCatchers are versatile active genetic elements that detect and 
            quantify inter-homolog somatic gene conversion.
  JOURNAL   Nat Commun. 2021 May 11;12(1):2625. doi: 10.1038/s41467-021-22927-1.
  PUBMED    33976171
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-11"; volume: "12(1):2625. doi"; pages: " 
            10.1038/s41467-021-22927-1"
COMMENT     Sequence Label: pUC19-whiteCopyCatcher
FEATURES             Location/Qualifiers
     source          1..7131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(207..226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(886..1677,1678..1746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1509..1528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1747..1851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1919..1937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3162..3215
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3216..3890
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3396..3416)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3808..3831
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3900..3920
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4061..4182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4098..4117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4152..4171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4185..4605
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        4625..4700
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    5000..5129
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     regulatory      5860..5869
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
       61 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1861 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     1921 taggcgtatc acgaggccct ttcgggtcat tatttggcta gacgaatgct ttgggctcag
     1981 catctgatat ctaggtatct tcgtgcgtat cttgctttaa attcttagca cctcggcttg
     2041 tataacaaaa taaataagtg agcacgatat gcatatctag ccccgggctc tttgaaacaa
     2101 ttttgaaaag tctcaaaaag ttatacaagg agataagaac tttaattctt ttgggaagta
     2161 agtaacgcag taaaggtaac aaagtattga aaaatatgat atgtatggaa tatttgaagc
     2221 catctttaat tatatgttcg ttgcatatat gtacatattg ggccgtttac gctctgatat
     2281 ttccctaatc atatcgagtg gtcgtcagct tttccgctga ataattgcgc ctccttcagt
     2341 atttcgttct catcaaagtg aatgactttt gagtgcactc attagccact cgcacaatcg
     2401 acaaaacggg aggaacaaca aattgagtcg cttctttatt cagtcatttc aatttgtcaa
     2461 gttcatcacg atactccttc gcctccctgc acagcgccat ctagcgccca ctcctcgagt
     2521 ttccttcgta tttcgtaagt aattcaaaaa tagcacgcaa agtaaataaa aaaaaaagta
     2581 agccaaaaaa ttaaataaat atgaaatgta gcatctttcg ctacttattt caacaaaata
     2641 cgaaaatagg gaaaaggtgg gagagtaaac cagaggaggc agggagataa gaaaacccag
     2701 tgggaaaggg acggcgaaaa cagaaaagaa aagaaaactt tttaataaca tttttcggcc
     2761 tcatttgttg tgaaaacaat ttcgccaggt ccctctatct ctttcccagc catatggcca
     2821 tctcgctctg ctcgaggagt gcctgaaaac ggatattaca ttttttcgcg tctacctctc
     2881 gtaacgtcac caattgtgct tttttttttg gtaacaggcc aaaaactgga cactgagtgg
     2941 ctagaagaat aagctgtcaa agcatcgcag cagaagaggg gcagtgaagc ctcgctagtc
     3001 catcatgatg gtcgactcta gaagtcgatc caacatggcg acttgtccca tccccggcat
     3061 gtttaaatat actaattatt cttgaactaa ttttaatcaa ccgatttatc tctcttccgc
     3121 aggtgggagg ttccggtgga agcggaggta gcggcggatc cgagggccgc ggcagcctgc
     3181 tgacctgcgg cgatgtggag gagaaccccg ggcccatggc ctcctccgag aacgtcatca
     3241 ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga
     3301 tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga
     3361 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     3421 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
     3481 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc
     3541 aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact
     3601 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     3661 gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg aagctgaagg
     3721 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc
     3781 tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca
     3841 ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg agatctcgac
     3901 ccaagaaaaa gcggaaggtg gaggacccgt aagatccacc ggatctagat aactgatcat
     3961 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4021 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tacctttttt gctcacctgt
     4201 gattgctcct actcaaatac aaaaacatca aattttctgt caataaagca tatttattta
     4261 tatttatttt acaggaaaga attcctttta aagtgtattt taacctataa tgaaaaacga
     4321 ttaaaaaaaa tacataaaat aattcgaaaa tttttgaata gcccaggttg ataaaaattc
     4381 atttcatacg ttttataact tatgccccta agtatttttt gaccatagtg tttcaattct
     4441 acattaattt tacagagtag aatgaaacgc cacctactca gccaagaggc gaaaaggtta
     4501 gctcgccaag cagagagggc gccagtgctc actacttttt ataattctca acttcttttt
     4561 ccagactcag ttcgtatata tagacctatt ttcaatttaa cgtcgcagtg aagcctcgct
     4621 aagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     4681 aagtggcacc gagtcggtgc ttttttgcct acctggagcc tgagagttgt tcaataaaat
     4741 aaaaatgttt cgtttttttg ctttcgccag tatttattat ttttcatcaa tatgtattca
     4801 atttggtatg tatttagtaa ttgtaatata tagacaatgg ttttccgttg acgtacatac
     4861 atctgacgtg tgtttattta gacataatag ttatgttttc acatcttttt aatgttcgct
     4921 taatgcgtat gcatttagac ctacgccccc aactgagaga actcaaaggt taccccagtt
     4981 ggggcactac gatcgatccc acgcgcttga aaggagtgtg taaatggaca atgttgttac
     5041 aataaaagtt gaaattattt tataaaattt attaaaaaca tatttaaaca taacatattg
     5101 tattaaaaca tgacaataca aactaagatt tagtcagata aacctatttg tagagttcgt
     5161 ccatacccaa agtaattcct gccgcggtca cgaactccag caacaccata tgatccctct
     5221 tttcgttggg atccttactg agtttactct gcgtgctcag gtagtggttg tcaggcaaga
     5281 gcacgggacc gtcgccgata ggtgtatttt gctgataatg atcggccaat tggaccgatc
     5341 catcttctat gttacaattc agaccgaaat tcgctttgat accgtttttc tgtttatcgg
     5401 ctgttatata aacatttcca tggatagcgt tatattccaa cttatggccc agaatattac
     5461 catcttcctt aaagtctata cccttcaact cgatacgatt caccagtgta tcaccttcaa
     5521 acttgacctc tgcccgtgtt ttgtagttgc catcgtcttt gaaaaatatt gtccgttcct
     5581 gcacataacc ttctggcatt gcacttttaa agaagtcgtg ctgcttcatg tggtccgggt
     5641 aacgagcgaa acactgaact ccccacgaga gtgtagtcac gagtgtcggc cagggtacag
     5701 gcagtttgcc cgtggtgcaa atgaatttca gggtcagttt accgtaagta gcatctcctt
     5761 caccttctcc actgacactg aatttgtgac cgttcacgtc accatccaat tcgacgagta
     5821 tcgggaccac gccagtgaag agttcttcac ctttactcac catggtggcg accggcttcg
     5881 agccgattgt ttagcttgtt cagctgcgct tgtttatttg cttagctttc gcttagcgac
     5941 gtgttcactt tgcttgtttg aattgaattg tcgctccgta gacgaagcgc ctctatttat
     6001 actccggcgg tcgagggttc gaaatcgata agcttggatc ctaattgaat tagctctaat
     6061 tgaattagtc tctaattgaa ttagatcccc gggcgagctc gaggaggaaa tgcaacagtt
     6121 ttcaggcttt ggtcttttag ctagacatac atatgtagca atgtaaacga taaatccaaa
     6181 tcccccaatc gttttgaaaa ataaatttaa ttttaaatag ttcaaaaaat gaaagttcta
     6241 agcagggctc gctgtagcaa ggaccgccag gcgctgagtg caaggtggac attatttttt
     6301 cttgctttta ttgaatgtca gctttttatt tggccctctt cttcggattt tgcctttctc
     6361 atttgcttgc atttttttgc taaatttttt ttatgattat ttatacaaat tattatgaga
     6421 gcagcggcgg ataaaatgct ttccatattg tggccatcat tttgtcttgt tttttccatt
     6481 ttatttttgc ctggacgcca attagtggat ccctgttatt tatgctttgc cgcaggccgc
     6541 tgcgatggca agaaacccca caaatggaaa acggaaaatt gtttatgaaa atgaaaatat
     6601 gcgcgaacgg aagtggtatg gggcgaaaag ttggggcggg gaggatggct tgatttgacc
     6661 ataccgcaaa cgtcttcggt taccgccatt tcgaaaaggg aagccccacc tgggcgaaaa
     6721 ctgacagctc agtgggtttg gaaatcactt tacccgtaaa caaaataacg taaataagaa
     6781 tttgtatttc ggatttataa acttaaactc aattgcagaa gtagtagttt gaacactatt
     6841 gcgttcaaca taattggatc aagtaaatgg actgctttat actgctcatt gcacttatct
     6901 acaagagatt gagttttccc accacccaaa aaaagccaaa gtatccttaa agttttatat
     6961 ttaataaaaa caaaactggc gaagctcaaa gtccccaaac gaaagccaaa gatgccagcg
     7021 atttacactc gcaattgtct tatgaggtgg caacatggtg gaggaacctt gccactgcca
     7081 ccacccatcg tcccatcgcc ccatcgtcca tcgcccaacg acctgtgacc c
//
