LOCUS       sequence-342234-        7099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CopyCatcher insertion donor for the pale locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Li Z, Marcel N, Devkota S, Auradkar A, Hedrick SM, Gantz VM, Bier E
  TITLE     CopyCatchers are versatile active genetic elements that detect and 
            quantify inter-homolog somatic gene conversion.
  JOURNAL   Nat Commun. 2021 May 11;12(1):2625. doi: 10.1038/s41467-021-22927-1.
  PUBMED    33976171
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-11"; volume: "12(1):2625. doi"; pages: " 
            10.1038/s41467-021-22927-1"
COMMENT     Sequence Label: pUC19-pleCopyCatcher
FEATURES             Location/Qualifiers
     source          1..7099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(207..226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(886..1677,1678..1746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1509..1528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1747..1851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1919..1937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3165..3218
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3219..3893
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3399..3419)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3811..3834
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3903..3923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4064..4185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4101..4120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4155..4174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4188..4608
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        4628..4703
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    5003..5132
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     regulatory      5863..5872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
       61 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1621 aactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1861 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     1921 taggcgtatc acgaggccct ttcgttgttc accccaaacg aaaacaaccc aaacacacaa
     1981 aacaaaatga tggccgttgc agcagcccaa aagaaccgcg agatgttcgc catcaagaaa
     2041 tcctacagta ttgaggtgag tcgaaaatcc agcaaaacat acaatttcca aatcgaattg
     2101 taaaaaaaaa gaagcaaatg agtgactaga tttggtctac gtgactatct aaaggttttg
     2161 tgttttcaaa aaaaaccagt taaccggcca gtagtcgagc ccatttttgt gatcccgcat
     2221 ttctttttcg ccaattcaat ttgaccaaac tcaatcctcg gttggccatt aagtcacacc
     2281 tctaagaaca ggcggcccaa atcgaaaggc ccaaatcgcc aacggacaca acgggttgtg
     2341 tgttaagtta ttatttaaca aataagtgaa agtggctgcc tttgggcccc atgcacttag
     2401 cctggccgaa acagtcgcgc ctcattgttg ttgactcgtc tctgtttatg tttatgctgc
     2461 gtccgagcaa aacaaaaaat atgaaatcaa tataaatgat tcaatgagtt ggtcatcttt
     2521 catcagccaa agcggtggcc caaaaaaaaa aaactgaaga aaacaaacaa aaaaaaaaaa
     2581 aaacgagctg acagtgaaaa ctgcaagtga tggaaacctt tttcccccca tcaagaaaaa
     2641 gaaagaaaaa aaacctttaa cgatcgccag ttgcattttt ttcccgccag aatcgcatgg
     2701 ggattttcga tttctcagtc acatataatt tatcttcgca cctgcccgct tcgaagcggc
     2761 gacatcgcat taaattacca ttcgtggctt tgaatcacaa tcaatacagt tccatgaata
     2821 gccagcttta ttcaaagaaa aagatctgaa gacggcgctt tcacacacca ttttatataa
     2881 tattttcaaa attgcgcaat actctgctca aagtcaccac ccaagcgtgg agccgaagaa
     2941 agccaccctg gcttgaaggt caaggcaatc gtctaatttc cgctccacat cctgccagtg
     3001 gtccatcatg atggtcgact ctagaagtcg atccaacatg gcgacttgtc ccatccccgg
     3061 catgtttaaa tatactaatt attcttgaac taattttaat caaccgattt atctctcttc
     3121 cgcaggtggg aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc
     3181 tgctgacctg cggcgatgtg gaggagaacc ccgggcccat ggcctcctcc gagaacgtca
     3241 tcaccgagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg
     3301 agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg
     3361 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
     3421 gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
     3481 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga
     3541 cccaggactc ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga
     3601 acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctccaccg
     3661 agcgcctgta cccccgcgac ggcgtgctga agggcgagac ccacaaggcc ctgaagctga
     3721 aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc
     3781 agctgcccgg ctactactac gtggacgcca agctggacat cacctcccac aacgaggact
     3841 acaccatcgt ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgagatctc
     3901 gacccaagaa aaagcggaag gtggaggacc cgtaagatcc accggatcta gataactgat
     3961 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4021 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4081 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4141 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaccttt tttgctcacc
     4201 tgtgattgct cctactcaaa tacaaaaaca tcaaattttc tgtcaataaa gcatatttat
     4261 ttatatttat tttacaggaa agaattcctt ttaaagtgta ttttaaccta taatgaaaaa
     4321 cgattaaaaa aaatacataa aataattcga aaatttttga atagcccagg ttgataaaaa
     4381 ttcatttcat acgttttata acttatgccc ctaagtattt tttgaccata gtgtttcaat
     4441 tctacattaa ttttacagag tagaatgaaa cgccacctac tcagccaaga ggcgaaaagg
     4501 ttagctcgcc aagcagagag ggcgccagtg ctcactactt tttataattc tcaacttctt
     4561 tttccagact cagttcgtat atatagacct attttcaatt taacgtcgaa gtccgtgtta
     4621 gtgacacgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     4681 aaaaagtggc accgagtcgg tgcttttttg cctacctgga gcctgagagt tgttcaataa
     4741 aataaaaatg tttcgttttt ttgctttcgc cagtatttat tatttttcat caatatgtat
     4801 tcaatttggt atgtatttag taattgtaat atatagacaa tggttttccg ttgacgtaca
     4861 tacatctgac gtgtgtttat ttagacataa tagttatgtt ttcacatctt tttaatgttc
     4921 gcttaatgcg tatgcattta gacctacgcc cccaactgag agaactcaaa ggttacccca
     4981 gttggggcac tacgatcgat cccacgcgct tgaaaggagt gtgtaaatgg acaatgttgt
     5041 tacaataaaa gttgaaatta ttttataaaa tttattaaaa acatatttaa acataacata
     5101 ttgtattaaa acatgacaat acaaactaag atttagtcag ataaacctat ttgtagagtt
     5161 cgtccatacc caaagtaatt cctgccgcgg tcacgaactc cagcaacacc atatgatccc
     5221 tcttttcgtt gggatcctta ctgagtttac tctgcgtgct caggtagtgg ttgtcaggca
     5281 agagcacggg accgtcgccg ataggtgtat tttgctgata atgatcggcc aattggaccg
     5341 atccatcttc tatgttacaa ttcagaccga aattcgcttt gataccgttt ttctgtttat
     5401 cggctgttat ataaacattt ccatggatag cgttatattc caacttatgg cccagaatat
     5461 taccatcttc cttaaagtct atacccttca actcgatacg attcaccagt gtatcacctt
     5521 caaacttgac ctctgcccgt gttttgtagt tgccatcgtc tttgaaaaat attgtccgtt
     5581 cctgcacata accttctggc attgcacttt taaagaagtc gtgctgcttc atgtggtccg
     5641 ggtaacgagc gaaacactga actccccacg agagtgtagt cacgagtgtc ggccagggta
     5701 caggcagttt gcccgtggtg caaatgaatt tcagggtcag tttaccgtaa gtagcatctc
     5761 cttcaccttc tccactgaca ctgaatttgt gaccgttcac gtcaccatcc aattcgacga
     5821 gtatcgggac cacgccagtg aagagttctt cacctttact caccatggtg gcgaccggct
     5881 tcgagccgat tgtttagctt gttcagctgc gcttgtttat ttgcttagct ttcgcttagc
     5941 gacgtgttca ctttgcttgt ttgaattgaa ttgtcgctcc gtagacgaag cgcctctatt
     6001 tatactccgg cggtcgaggg ttcgaaatcg ataagcttgg atcctaattg aattagctct
     6061 aattgaatta gtctctaatt gaattagatc cccgggcgag ctcgcctagg tcactaacac
     6121 ggacttcaat cttttcgctt tcgtttccag aatggctatc catcccgccg tcgcagcctg
     6181 gtggatgatg cccgtttcga gaccctggtg gtcaagcaga ccaaacaaac cgtcctcgag
     6241 gaggcccgca gcaaggcaaa tggtaagtga atagggtcca acaagtgggt cactaaaata
     6301 gctccaaaga gggtttctat ttgggatcaa aagggaaatt ttgcagctgg ggctttacct
     6361 gttgattaaa aacttaaaaa tgtgcaattt ttatttcatt ttattcgtgt tgacttagat
     6421 ttgcgttttt ttcttattca ttttaaccct ttatttatta tttgccttcg ttcttatttt
     6481 tatgatttta aagacgattc cctggaagat tgcattgtgc aggctcagga gcacattccc
     6541 tccgagcaag atgtggagct ccaggacgag catgccaatc tggagaacct gcccttggag
     6601 gaatatgttc caggttaata tccatttcca tttcaattcc catgccagtt actcctcttt
     6661 ctgagcctat tatctttgat ttgattttga ttatgatttt catgggcttt attattattt
     6721 ttatttcatt cgcttttagt tgaggaggat gttgagtttg agagcgttga acaggagcag
     6781 tcggagtcgc agtcgcagga gccagaaggc aaccaacagc cgaccaagaa cggtgagcag
     6841 ggggttctag aatccagcag ccaaccataa gtagtactta acttctgaga ggggcttttg
     6901 aaaagcgtgc gacattgatt taagtgctta atatgataaa gtgccagggc agaggaaaag
     6961 cactcagcca cacggacaat tgtgagtggg tgcgctcgct cgctctctct ctctctctct
     7021 ctctctgtgg aaatgtcgca tttccctttt tcatttgtta cgctcgactt aatacaagta
     7081 cgaaatgcgt ttgcctggc
//
