LOCUS       sequence-342247-        7988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at 
            amino acid 217.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R,
            Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, 
            Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, 
            McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, 
            Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
  TITLE     The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
  JOURNAL   Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7.
            Epub 2021 Apr 14.
  PUBMED    33854239
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03474-7"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "794-798"
COMMENT     Sequence Label: pPB-AMBRA1-217*-3xFLAG
FEATURES             Location/Qualifiers
     source          1..7988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        337..1515
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          568..1506
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1463..1483
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1515..1539
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2212..2277
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2281..2305)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2383..2402)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2448..2503
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2502..2521)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        2858..3237
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3238..3441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3392..3412
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3474..4268
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3474..4268
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3528..3547)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4138..4157
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4306..4323)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4312..4536
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4886..4948
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5014..5033)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5015..5033)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5040..5057)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5040..5056)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5049..5071)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5198..5653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5285..5304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5495..5516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5679..5783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5784..5852,5853..6644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6002..6021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6815..7403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7304..7323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7557..7574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7691..7712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7727..7732,7733..7750,7751..7757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7770..7792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7789..7805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7789..7805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7826..7844
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca
      361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      541 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      601 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      661 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      721 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
      781 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      841 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
      901 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
      961 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1021 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc
     1081 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1141 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1201 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1261 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1321 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1381 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1441 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1501 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgaa ggttgtccca
     1561 gaaaagaatg ctgtccggat actctggggg cgagaacggg gtgctcgggc catgggagct
     1621 cagcggcttc tgcaggagct ggtagaggat aaaacccggt ggatgaaatg ggagggcaag
     1681 agagtagaac tgccggattc accacgctct accttcttat tggccttcag cccagacagg
     1741 actctcttag ccagcaccca tgtgaaccat aatatctata ttacggaggt gaagactggc
     1801 aagtgtgttc attccctgat tggacaccgc cgtactccat ggtgtgtcac ttttcatccc
     1861 accatctcag gccttattgc ttctggctgc ctagatgggg aggttaggat ttgggattta
     1921 cacggtggca gtgaaagctg gttcacagat agcaacaatg ccattgcctc cctggctttc
     1981 caccctacgg ctcagctcct gctgattgcc actgccaatg agatccactt ctgggactgg
     2041 agtcgacggg aaccctttgc tgtggtgaag acagctagtg agatggaacg ggtccgtctg
     2101 gtgagatttg atccacttgg acactactta ctcacagcaa ttgttaaccc ctctaatcaa
     2161 cagggtgatg acgaaccaga gatccccata gatggcagcg gcagcggcag cgactacaaa
     2221 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag
     2281 acccagcttt cttgtacaaa gtggtgatcc tcaggtgcag gctgcctatc agaaggtggt
     2341 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     2401 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     2461 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2521 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     2581 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     2641 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     2701 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     2761 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     2821 atggagatcc ctcgacctgc agcccaagct tcgcgttgac attgattatt gactagttat
     2881 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2941 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3001 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3061 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3121 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3181 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3241 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3301 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3361 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3421 gaggtctata taagcagagc tctctggcta actagagaac ccactgcgcc accatgattg
     3481 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3541 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3601 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     3661 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3721 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3781 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3841 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3901 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3961 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4021 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4081 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4141 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4201 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4261 tcttctgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4321 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4381 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4441 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4501 atagcaggca tgctggggat gcggtgggct ctatggctcg agttaattaa cgagagcata
     4561 atattgatat gtgccaaagt tgtttctgac tgactaataa gtataatttg tttctattat
     4621 gtataggtta agctaattac ttattttata atacaacatg actgttttta aagtacaaaa
     4681 taagtttatt tttgtaaaag agagaatgtt taaaagtttt gttactttat agaagaaatt
     4741 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     4801 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     4861 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
     4921 acgtcacaat atgattatct ttctagggtt aaataatagt ttctaatttt tttattattc
     4981 agcctgctgt cgtgaatacc gagctccaat tcgccctata gtgagtcgta ttacaattca
     5041 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5101 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5161 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5401 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5641 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6001 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7621 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7681 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7741 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7801 atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac
     7861 ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg tcacacagct tggccacaat
     7921 gtggtttttg tcaaacgaag attctatgac gtgtttaaag tttaggtcga gtaaagcgca
     7981 aatctttt
//
