LOCUS       sequence-342248-        8840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at 
            amino acid 501.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R,
            Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, 
            Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, 
            McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, 
            Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
  TITLE     The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
  JOURNAL   Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7.
            Epub 2021 Apr 14.
  PUBMED    33854239
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03474-7"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "794-798"
COMMENT     Sequence Label: pPB-AMBRA1-501*-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        337..1515
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          568..1506
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1463..1483
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1515..1539
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3064..3129
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3133..3157)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3235..3254)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3300..3355
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3354..3373)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3710..4089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4090..4293
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4244..4264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4326..5120
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4326..5120
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4380..4399)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4990..5009
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5158..5175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5164..5388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5738..5800
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5866..5885)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5867..5885)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5892..5909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5892..5908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5901..5923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6050..6505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6137..6156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6347..6368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6531..6635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6636..6704,6705..7496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6854..6873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7667..8255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8156..8175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8409..8426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8543..8564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8579..8584,8585..8602,8603..8609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8617..8633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8622..8644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8641..8657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8641..8657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8678..8696
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca
      361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      541 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      601 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      661 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      721 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
      781 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      841 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
      901 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
      961 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1021 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc
     1081 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1141 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1201 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1261 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1321 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1381 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1441 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1501 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgaa ggttgtccca
     1561 gaaaagaatg ctgtccggat actctggggg cgagaacggg gtgctcgggc catgggagct
     1621 cagcggcttc tgcaggagct ggtagaggat aaaacccggt ggatgaaatg ggagggcaag
     1681 agagtagaac tgccggattc accacgctct accttcttat tggccttcag cccagacagg
     1741 actctcttag ccagcaccca tgtgaaccat aatatctata ttacggaggt gaagactggc
     1801 aagtgtgttc attccctgat tggacaccgc cgtactccat ggtgtgtcac ttttcatccc
     1861 accatctcag gccttattgc ttctggctgc ctagatgggg aggttaggat ttgggattta
     1921 cacggtggca gtgaaagctg gttcacagat agcaacaatg ccattgcctc cctggctttc
     1981 caccctacgg ctcagctcct gctgattgcc actgccaatg agatccactt ctgggactgg
     2041 agtcgacggg aaccctttgc tgtggtgaag acagctagtg agatggaacg ggtccgtctg
     2101 gtgagatttg atccacttgg acactactta ctcacagcaa ttgttaaccc ctctaatcaa
     2161 cagggtgatg acgaaccaga gatccccata gatggaacag aattatccca ctaccgtcag
     2221 cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc tcctccacaa tttcctgcac
     2281 atgctgtcct cccgctcttc tggcatccag accgagccct tccatccccc ggagcaggcc
     2341 tcgtcaacgc agcaggacca gggcctcctg aaccggccgt ctgccttcag tacagtccag
     2401 agcagcactg ccggcaacac gctccgcaac ctcagtctgg gtcctacccg ccgctctttg
     2461 ggagggcctc tgtctagcca cccttctagg tatcaccgag aaatagctcc tgggttgaca
     2521 ggatctgagt ggacccggac agtactcagt ctgaactccc gctctgaggc ggaatccatg
     2581 cccccgccca gaaccagtgc ctcttcggtg agtttgctgt ctgtgctgag acagcaggaa
     2641 ggtggctctc aggcatctgt gtacacttca gccacagaag ggaggggttt tccggcatca
     2701 gggttggcaa ctgagtcaga tggagggaat ggctccagcc aaaacaactc gggcagcatt
     2761 cgccatgagc ttcagtgtga cctgagacgc ttctttctgg agtatgaccg gcttcaggag
     2821 ctggatcaga gcctgagtgg ggaagctccc cagacccaac aggcccagga aatgctcaac
     2881 aataacattg aatctgagag gccaggccct tcccaccagc ccaccccaca cagcagtgag
     2941 aacaactcca acctgtcccg tggccacctg aatcgctgtc gtgcttgcca caatctcctg
     3001 accttcaaca acgataccct gcgctgggaa agaaccacac ctaacggcag cggcagcggc
     3061 agcgactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     3121 gatgacaagt agacccagct ttcttgtaca aagtggtgat cctcaggtgc aggctgccta
     3181 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     3241 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     3301 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     3361 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     3421 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     3481 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     3541 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     3601 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     3661 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttcgcgttg acattgatta
     3721 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     3781 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     3841 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     3901 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     3961 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4021 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4081 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4141 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4201 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4261 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgcg
     4321 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4381 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4441 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4501 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4561 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4621 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4681 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4741 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4801 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4861 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4921 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4981 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5041 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5101 ttcttgacga gttcttctga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     5161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5341 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct cgagttaatt
     5401 aacgagagca taatattgat atgtgccaaa gttgtttctg actgactaat aagtataatt
     5461 tgtttctatt atgtataggt taagctaatt acttatttta taatacaaca tgactgtttt
     5521 taaagtacaa aataagttta tttttgtaaa agagagaatg tttaaaagtt ttgttacttt
     5581 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
     5641 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
     5701 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
     5761 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaaataata gtttctaatt
     5821 tttttattat tcagcctgct gtcgtgaata ccgagctcca attcgcccta tagtgagtcg
     5881 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5941 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6001 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6061 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6121 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6181 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6241 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6301 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6361 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6421 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6481 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8521 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8581 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8641 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     8701 aaaagctggt acctcgcgcg acttggtttg ccattcttta gcgcgcgtcg cgtcacacag
     8761 cttggccaca atgtggtttt tgtcaaacga agattctatg acgtgtttaa agtttaggtc
     8821 gagtaaagcg caaatctttt
//
