LOCUS       sequence-342250-        1569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NanoLuc enzyme fused to LAF-1 RGG domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Guan M, Garabedian MV, Leutenegger M, Schuster BS, Good MC, Hammer 
            DA
  TITLE     Incorporation and Assembly of a Light-Emitting Enzymatic Reaction 
            into Model Protein Condensates.
  JOURNAL   Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373.
  PUBMED    34648259
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373."
COMMENT     Sequence Label: RGG-NanoLuc-RGG
FEATURES             Location/Qualifiers
     source          1..1569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             511..1023
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1549..1566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaatcca atcaatccaa taacggtggc tcgggtaatg ccgctctgaa tcgtggtggc
       61 cgctacgtgc cgccgcatct gcgtggtggc gatggtggtg cagcagcagc tgcatctgct
      121 ggcggtgatg accgtcgcgg cggtgcgggc ggtggcggtt atcgtcgcgg cggtggcaat
      181 agtggtggcg gtggcggtgg cggttatgat cgtggttaca atgacaaccg tgatgaccgc
      241 gataaccgtg gcggttccgg cggttacggt cgtgatcgta attatgaaga ccgtggttac
      301 aacggcggtg gcggtggcgg tggcaatcgt ggctataaca ataaccgtgg tggcggtggc
      361 ggtggctaca atcgccagga tcgtggtgac ggtggcagct ctaacttttc gcgcggtggc
      421 tataataacc gtgatgaagg cagcgacaac cgcggtagtg gccgttccta caataacgat
      481 cgtcgcgaca atggtggcga tggcgaattc atggtcttca cactcgaaga tttcgttggg
      541 gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc
      601 agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt
      661 gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac
      721 caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt
      781 aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac
      841 tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca
      901 gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc
      961 ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg
     1021 gcgacattcg ggaagcttat ggaatccaat caatccaata acggtggctc gggtaatgcc
     1081 gctctgaatc gtggtggccg ctacgtgccg ccgcatctgc gtggtggcga tggtggtgca
     1141 gcagcagctg catctgctgg cggtgatgac cgtcgcggcg gtgcgggcgg tggcggttat
     1201 cgtcgcggcg gtggcaatag tggtggcggt ggcggtggcg gttatgatcg tggttacaat
     1261 gacaaccgtg atgaccgcga taaccgtggc ggttccggcg gttacggtcg tgatcgtaat
     1321 tatgaagacc gtggttacaa cggcggtggc ggtggcggtg gcaatcgtgg ctataacaat
     1381 aaccgtggtg gcggtggcgg tggctacaat cgccaggatc gtggtgacgg tggcagctct
     1441 aacttttcgc gcggtggcta taataaccgt gatgaaggca gcgacaaccg cggtagtggc
     1501 cgttcctaca ataacgatcg tcgcgacaat ggtggcgatg gcctcgagca ccaccaccac
     1561 caccactga
//
