LOCUS       sequence-342252-        2310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SmBit fused to LAF-1 RGG domain with N-terminal MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Guan M, Garabedian MV, Leutenegger M, Schuster BS, Good MC, Hammer 
            DA
  TITLE     Incorporation and Assembly of a Light-Emitting Enzymatic Reaction 
            into Model Protein Condensates.
  JOURNAL   Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373.
  PUBMED    34648259
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373."
COMMENT     Sequence Label: MBP-RGG-SmBit-RGG
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1101
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1075..1098
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1132..1155
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2290..2307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt
       61 ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat
      121 ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt
      181 atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc
      241 accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac
      301 aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa
      361 gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
      421 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
      481 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa
      541 gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt
      601 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
      661 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
      721 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt
      781 ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc
      841 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
      901 ggtgccgtag cgctgaagtc ttacgaggaa gagttggtga aagatccgcg tattgccgcc
      961 actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
     1021 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
     1081 gccctgaaag acgcgcagac cggtggggga gggagtggcg gtggtggcag ccttgaagtc
     1141 ctctttcagg ggccgttgga atccaatcaa tccaataacg gtggctcggg taatgccgct
     1201 ctgaatcgtg gtggccgcta cgtgccgccg catctgcgtg gtggcgatgg tggtgcagca
     1261 gcagctgcat ctgctggcgg tgatgaccgt cgcggcggtg cgggcggtgg cggttatcgt
     1321 cgcggcggtg gcaatagtgg tggcggtggc ggtggcggtt atgatcgtgg ttacaatgac
     1381 aaccgtgatg accgcgataa ccgtggcggt tccggcggtt acggtcgtga tcgtaattat
     1441 gaagaccgtg gttacaacgg cggtggcggt ggcggtggca atcgtggcta taacaataac
     1501 cgtggtggcg gtggcggtgg ctacaatcgc caggatcgtg gtgacggtgg cagctctaac
     1561 ttttcgcgcg gtggctataa taaccgtgat gaaggcagcg acaaccgcgg tagtggccgt
     1621 tcctacaata acgatcgtcg cgacaatggt ggcgatggcg aattcggttc tagtggtggt
     1681 ggtggttctg gtggtggtgg ttctagtggt gtgacaggtt atagactttt tgaagaaatt
     1741 ctaggaggag gatcgggagg ctctgaattc gggaagctta tggaatccaa tcaatccaat
     1801 aacggtggct cgggtaatgc cgctctgaat cgtggtggcc gctacgtgcc gccgcatctg
     1861 cgtggtggcg atggtggtgc agcagcagct gcatctgctg gcggtgatga ccgtcgcggc
     1921 ggtgcgggcg gtggcggtta tcgtcgcggc ggtggcaata gtggtggcgg tggcggtggc
     1981 ggttatgatc gtggttacaa tgacaaccgt gatgaccgcg ataaccgtgg cggttccggc
     2041 ggttacggtc gtgatcgtaa ttatgaagac cgtggttaca acggcggtgg cggtggcggt
     2101 ggcaatcgtg gctataacaa taaccgtggt ggcggtggcg gtggctacaa tcgccaggat
     2161 cgtggtgacg gtggcagctc taacttttcg cgcggtggct ataataaccg tgatgaaggc
     2221 agcgacaacc gcggtagtgg ccgttcctac aataacgatc gtcgcgacaa tggtggcgat
     2281 ggcctcgagc accaccacca ccaccactga
//
