LOCUS       sequence-34226-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1616-HQ858853
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      299..308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatgga gagcttgtgt gggcagcttg agcatgaaca tgagcagcag
      181 ctgcaccaat cctcctcatc ttcacttctc ccctactgct tcctttctgc tccagagcct
      241 cttcctgtcc caggcttccc tgccggctgc agcaaccttg aagagaaggc agcagcagcc
      301 atggcggcct atgagtatga gagcagcagc tgcagctccc ttgaccccac ctcaatgcca
      361 atggtgtact caccaatagt gctgcagcca caggagtgcc ctctttcctt tgtctttgac
      421 aatgccgctg ccgctgccgg cgacaacaaa tgggtgcctg gcattcaagg aagttgccct
      481 tgttcattgg gctcaactca agatatggat gcaagttggg gcaagagtag aaagcacaag
      541 aggagcaatg tgggcttgaa gggacttgag gagaaaaagg caagaagggt ggtactacat
      601 caacatgatg atgatgtgaa gaagaaggcc aaggaggcag caggtgggga gccaccagct
      661 gggtacattc atgtgagggc aaggagaggc caagcaacag acagccacag ccttgctgag
      721 agggtgagaa gggagaagat cagtgagagg atgaagatgc tgcaaagcct ggttcctggt
      781 tgtgacaagg tcacagggaa agccctcatg ctagatgaga tcatcagcta tgtgcaatct
      841 ctgcagaacc aagttgagtt tctgtccatg aagcttgcct cccttagccc tttgatgtat
      901 gagtttggac cgggcatgaa atg
//
