LOCUS       sequence-342265-       16699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16699)
  AUTHORS   Williams LK, Mackay DR, Whitney MA, Couldwell GC, Sundquist WI, 
            Ullman KS
  TITLE     Identification of abscission checkpoint bodies as structures that 
            regulate ESCRT factors to control abscission timing.
  JOURNAL   Elife. 2021 Aug 4;10. pii: 63743. doi: 10.7554/eLife.63743.
  PUBMED    34346309
REFERENCE   2  (bases 1 to 16699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-08-4"; volume: "10. pii: 63743. doi"; pages: " 
            10.7554/eLife.63743"
COMMENT     Sequence Label: pLVX-SRRM2-mCherry
FEATURES             Location/Qualifiers
     source          1..16699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1..20)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     497..517
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    617..1205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(670..690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1088..1099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1412..2045
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2173..2189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2173..2189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2186..2208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2197..2213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2221..2227,2228..2245,2246..2251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2266..2287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2404..2421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2575..3163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2655..2674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3334..4125,4126..4194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3957..3976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4195..4299)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4347..4481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4384..4403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4438..4457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4649..5282
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5329..5454
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5951..6184
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6369..6413
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6676..6791
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        6854..7168
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    6862..6880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6898..6916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6933..6951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6969..6987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7005..7023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7040..7058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7076..7094
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7141..7165
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      15453..15462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15459..16169
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(15604..15622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        16180..16679
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16215..16238)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     16612..16631
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
       61 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
      121 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
      181 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
      241 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
      301 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
      361 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
      421 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
      481 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
      541 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
      601 cgggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
      661 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
      721 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
      781 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
      841 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
      901 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
      961 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     1021 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1081 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     1141 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     1201 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     1261 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     1321 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     1381 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     1441 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     1501 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     1561 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     1621 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     1681 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     1741 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     1801 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     1861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2041 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     2101 atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt
     2161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3001 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4321 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4441 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     4501 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     4561 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     4621 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     4681 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     4741 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     4801 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     4861 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     4921 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     4981 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5041 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     5101 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5161 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5221 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5281 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     5341 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     5401 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     5461 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5521 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5581 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5641 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5701 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5761 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     5821 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     5881 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     5941 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6001 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6061 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6121 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6181 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6241 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     6301 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     6361 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     6421 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     6481 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     6541 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     6601 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     6661 ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     6721 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     6781 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg atgaggccct
     6841 ttcgtcttca ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     6901 ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc
     6961 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     7021 agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct
     7081 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
     7141 agctcgttta gtgaaccgtc agatcgcctg gagaaggatc cgccaccatg tacaacggga
     7201 tcgggctgcc gacgccccgg ggcagcggca ccaacggcta cgtccagcgc aacctgtccc
     7261 tggtgcgggg ccgccggggt gagcggcctg actacaaggg agaggaggaa ctgcggcgcc
     7321 tggaggctgc cctggtgaag cggcctaatc ctgacatcct ggaccacgag cgcaagcggc
     7381 gcgtcgagct gcgatgcctc gagctggagg agatgatgga agagcagggg tacgaggaac
     7441 agcaaattca ggaaaaagtg gcgacctttc gactcatgtt gctggagaag gatgtgaacc
     7501 ctgggggcaa ggaggagacc ccagggcaga ggccagcggt cacggagact caccagttgg
     7561 cagaattaaa tgagaagaag aatgaaagac tccgtgctgc ctttggcatc agtgattctt
     7621 acgtagatgg cagctctttt gatcctcagc gtcgtgcccg agaagctaaa caaccagctc
     7681 ctgagcctcc caaaccttac agccttgttc gggagtctag cagttctcgc tcaccaaccc
     7741 caaagcagaa gaagaagaaa aagaagaaag atagaggacg caggtcagag agcagctctc
     7801 ctcgacggga gagaaagaaa agctcaaaga agaagaagca caggtcagaa tctgagtcca
     7861 agaaacgtaa gcataggtct cccactccaa agagcaaacg taaatctaag gacaaaaagc
     7921 gaaagcggtc tcgaagtaca acaccagccc ccaagagccg ccgggcccac cgttcaactt
     7981 ctgctgactc tgcttcctcc tccgatactt cccgcagtcg gtctcgaagt gctgcagcta
     8041 aaactcatac aactgccttg gctgggcgaa gtccttcccc tgcttcaggg cgacgcgggg
     8101 agggagatgc gcctttcagt gaaccaggta ctaccagcac acaacggcct agtagcccgg
     8161 agactgctac gaaacagcct agcagccctt atgaagacaa agataaagac aagaaggaga
     8221 aatctgcaac tcgacctagc ccctctccgg aaaggagcag cacaggccca gaaccacctg
     8281 ctcccactcc gctccttgct gagcgacatg gcggctcccc acaacccctt gcaaccaccc
     8341 ccttaagcca ggagccagtg aaccccccat ctgaggcctc tccaactcgg gaccgttcac
     8401 cacctaagtc tcccgagaaa cttccccagt cttcttcctc agagagcagc ccaccatccc
     8461 ctcaacctac caaagtttct cggcatgcca gctcttcccc agaaagtcct aaacctgctc
     8521 cagctccagg gtcccaccga gagatttctt cttctcccac atctaagaat cgctcacatg
     8581 gccgagcaaa acgggataaa tcacattctc ataccccctc ccgtaggatg gggaggtccc
     8641 gtagccctgc caccgctaag agagggcgat ctcggtctcg aacccctacc aagagaggtc
     8701 attctcgatc ccgatctccc cagtggcgta ggtccaggtc tgcacagagg tggggaagat
     8761 ctagaagccc ccagcgacgt ggccgctcta ggtctcctca gcgaccaggc tggtctagga
     8821 gcagaaatac ccagagaaga ggcaggtcta ggtcagcaag gcgagggagg tcccactcta
     8881 gatccccagc cactaggggt agatctcgtt ctagaacacc agcccgccgg ggcaggtccc
     8941 gctctagaac acctgccagg cggagatcac gatccagaac tcccaccagg cgtaggtctc
     9001 ggtctagaac accagcccgg aggggcaggt ctcggtctag aacacctgct aggcgcagat
     9061 ctaggacccg atcaccagta cgacgcaggt ctcgtagtag atcaccagcc aggagaagtg
     9121 gcaggtcacg ctctagaacc ccagctagac gtggccgctc acgctccaga accccagcca
     9181 gacgtggccg ctcacgctct agaaccccag ctagacgcag tggtcgctca cgctccagaa
     9241 caccagccag gagagggagg tctcggtcta ggacaccaag acgaggaaga tcccgcagta
     9301 gaagcttagt tagacgtgga agatctcact ctagaacacc tcaaagaaga ggcagatctg
     9361 gctcatcttc agagcggaaa aacaaatcca gaacatctca aagaagaagc aggtccaatt
     9421 caagcccaga aatgaagaaa tctcgcattt cttcaaggcg gagcaggtct ctctcttcac
     9481 cacggtccaa agcaaaatct cgcttgtctt tgaggcgcag cctttcaggg tcttccccat
     9541 gccctaaaca aaagtcacag acaccaccca ggcgcagtcg ctctggatcc tcccaaccta
     9601 aagctaaatc tagaacgcca cccagacgca gtcgctccag ttcttctccg ccacctaaac
     9661 agaaatctaa gacaccatca agacaaagtc attccagttc atctcctcat cctaaagtga
     9721 aatctggaac accaccgagg caagggtcca taacaagtcc ccaggccaat gagcaatctg
     9781 taacgccaca gagacggagc tgttttgaat catcacctga ccctgagttg aaatctagga
     9841 ccccttctag acatagctgc tcagggtcct ctcctcctag agtgaaatct agcacacctc
     9901 ccagacagag cccatctagg tcatcatctc cacaacccaa agtgaaggca ataatatcac
     9961 caagacaaag aagccattct ggctcctctt ctccaagtcc tagtagggtg acgtcgagaa
    10021 caactccacg gcgaagcaga tcagtatctc cctgctccaa tgtggaatcc agattgttgc
    10081 caagatacag tcattctggg tcctcctcac cagataccaa agtgaaacct gaaacaccgc
    10141 caagacaaag tcactcaggg tctatttcac cataccccaa agtaaaggcc caaactccac
    10201 cggggccaag tctttctgga tcaaagtcac catgtcccca agagaagtct aaagactcac
    10261 tagttcaaag ttgccctgga tccctctctc tctgtgcagg agtaaaatct agcacaccac
    10321 caggcgagag ctattttggt gtctcatctc tgcaactgaa aggacaatct caaacttcac
    10381 cagaccacag atctgatact tcaagtccag aagtgagaca gagtcattca gaatcaccat
    10441 ctctgcagag caaatctcaa acatcaccta agggaggtcg gtccaggtct tcatctccag
    10501 tcactgagct ggcatccaga tctccaataa gacaagatag aggtgagttc tcagcgagtc
    10561 ctatgttgaa atctggaatg tctcctgagc agagcaggtt ccagtctgac tcttcttcat
    10621 atcctacagt ggactcgaat tctctcttgg ggcagagtag attggagact gctgaatcaa
    10681 aagagaaaat ggccttaccc cctcaggagg atgctactgc atcacctcct agacagaaag
    10741 acaaatttag tccctttcca gtacaggata ggcctgagtc ttcactggta ttcaaagaca
    10801 cacttagaac cccgccaagg gaaagaagtg gtgctgggtc atctccagaa acaaaagagc
    10861 aaaatagtgc attgcctacg tcaagccaag atgaagagtt aatggaggtg gtagagaagt
    10921 ctgaagaacc cgcaggccaa atcctgtctc atttgtcttc agaacttaaa gaaatgtcca
    10981 caagtaactt tgaatcatct cctgaagtag aagaaaggcc tgctgtgtct ttgactcttg
    11041 atcagagcca gtcacaggct tctttggaag cagtagaagt cccttcaatg gcctcatctt
    11101 ggggtgggcc acatttttct ccagaacata aagaactgtc taactcccca ctcagggaga
    11161 acagctttgg atcaccttta gaatttagaa actcaggccc acttggtaca gaaatgaata
    11221 ctggattttc ttctgaggtt aaagaagatt tgaatggacc gtttcttaat cagctggaaa
    11281 cagatccatc tctagacatg aaagaacaat cgacaagatc ctctggacac agcagttctg
    11341 agttatcccc agatgcagtg gaaaaggcag ggatgtcttc aaatcagagc atctcttcac
    11401 ctgtgcttga tgctgtaccc agaacaccct cgagagaaag aagtagttct gcatcttctc
    11461 ctgaaatgaa agatggttta cccagaactc catcaaggag aagcaggtct gggtcttctc
    11521 caggacttag agatgggtct gggactccct cgaggcacag cctgtctggg tcctctcctg
    11581 gaatgaaaga tatacctaga acgccatcta gagggagaag cgaatgtgat tcttccccag
    11641 aaccgaaagc tttgcctcag actcctaggc cgaggagtcg ttctccatca tccccagagc
    11701 tcaacaacaa gtgtcttacc ccccagagag aaagaagcgg gtcagaatca tcagttgatc
    11761 agaaaactgt ggctcggact cccctggggc agagaagtcg ttcgggatcc tctcaagaac
    11821 ttgatgtgaa acccagtgca tcccctcagg aaagaagtga gtcagactct tctccagatt
    11881 ctaaagccaa gacaagaacc ccacttcggc agaggagtcg gtctggatca tctccagagg
    11941 ttgacagcaa atctcgacta tcccctcggc gcagtaggtc tggttcctcc cctgaagtga
    12001 aagataagcc aagagcagca cccagggcac agagtggttc tgattcctct cctgaaccta
    12061 aagctccagc ccctcgggcc cttcccagac gaagcagatc aggttcatca agcaaaggca
    12121 gaggcccttc tcctgaagga agcagcagta ccgagtcctc tcctgaacat ccgcccaaat
    12181 ccagaactgc tcgcagaggt tccaggtcat caccagagcc caagaccaag tctcgtacac
    12241 cacctcgacg tcgcagctct cgatcatctc cggagctaac aaggaaggcc agactgtccc
    12301 gtagaagccg ctctgcctca tcctcaccag aaactcgctc tagaactccc ccaaggcacc
    12361 ggagaagtcc ctcagtgtct tccccggagc cagccgaaaa atcgaggtct tcacgccgac
    12421 ggcgctcagc ttcatctcca cgcactaaga caacctcaag gagaggccgc tctccttcgc
    12481 caaagcctcg tggactccag aggtcccgtt cccgctcaag gagagagaaa acaagaacaa
    12541 cccgacgtcg agataggtct ggatcttctc agtcaacctc tcggcgaaga cagcggagcc
    12601 ggtcaaggtc gcgggttact cggcggcgga ggggaggctc tggttatcac tcaaggtcac
    12661 ctgcccggca ggaaagttcc cggacctcct ctcgacgccg aagaggccgc tctcggacac
    12721 ccccaaccag tcggaagcgt tctcgctcac gcacatcacc agccccgtgg aaacgctcta
    12781 gatctcgagc ctctccagcc actcaccggc gatccaggtc cagaaccccc ctgataagcc
    12841 gacgtaggtc cagatctcga acttcaccag tcagccggag acggtcaagg tccaggactt
    12901 cagtgactcg acgaagatcc cggtcaagag catccccagt gagcagaagg cgatccagat
    12961 ccagaacgcc accagtaacc cgccgtcgtt caaggtctag aacgccaaca acacgccgcc
    13021 gctcccgttc tagaactcca ccagtgactc gcagaaggtc cagatccagg actccaccag
    13081 taaccaggag gcgatctcga agcagaactt cgcctatcac tcgcagaaga tcaagatcca
    13141 gaacatctcc ggtcacccga aggagatctc gatctcgcac atctccagta actcgaagaa
    13201 ggtcccgctc tcgaacctca ccagtgacac gccgccgctc taggtcccgg acacctccag
    13261 ctattcggcg ccgctctaga tctcgaacgc cactgttacc acgcaaacgt tctcgaagtc
    13321 gctcaccact tgctatccgc cgccgctcca gatcccgtac tccacgaaca gctcggggta
    13381 aacggtcctt aacaagatct cctccagcca tccgcaggcg ttctgcatct ggaagtagtt
    13441 ctgatcgttc acgatctgct actcctccag caacaagaaa tcattctggt tcacggacac
    13501 ctccagtagc actcaacagt tccagaatga gctgcttcag tcgtcctagc atgtccccaa
    13561 cacctcttga tcgctgcaga tcacctggaa tgcttgaacc ccttggcagc tctagaacac
    13621 ccatgtctgt cctgcagcaa gccggcggct ccatgatgga tggtccaggt ccccgaatac
    13681 ctgaccacca gagaacatct gtgccagaaa atcatgctca gtccaggatt gcacttgccc
    13741 tgacagctat cagtcttggc accgctcggc ctcctccgtc catgtctgct gctggccttg
    13801 ctgcaagaat gtcccaggtt ccagccccgg tgcctctcat gagtctcaga accgcaccag
    13861 cagccaacct tgccagcagg attcctgcag cctctgcggc agccatgaac ctagccagcg
    13921 ccaggacacc tgccattcca acagcagtga acctggctga ctctcgaacg ccagctgcag
    13981 cagcggccat gaacttggcc agccccagaa cagcggtggc accttcggct gtgaacctgg
    14041 ctgaccctcg cactcccaca gccccagctg tgaacctagc aggggccaga accccagctg
    14101 ccttggcagc tctgagtctc acaggctctg gcacaccacc aactgctgca aactatccct
    14161 ccagctccag aacaccacag gctccagcct ctgcaaacct ggtgggtcct cggtctgcac
    14221 atgccacagc tcctgtgaat attgccggct ccagaaccgc cgcagccttg gcccccgcga
    14281 gcctcaccag tgctaggatg gctccagcat tgtctggtgc aaacctcacc agccccaggg
    14341 tgcccctttc tgcctacgag cgtgtcagtg gcagaacctc accaccgctc cttgaccgag
    14401 ctaggtccag aacaccaccg tctgccccaa gccaatctag gatgacctct gaacgggctc
    14461 cctccccttc ctctagaatg ggccaggctc cttcacagtc tcttctccct ccagcacagg
    14521 atcagccgag gtctcctgtg ccttctgctt tttcagacca atcccgttgt ttgattgccc
    14581 agaccacccc tgtagcaggg tctcagtccc tttcctctgg ggcagtggca acgaccacgt
    14641 cctctgctgg tgatcacaat ggcatgctct ctgtccctgc ccctggggtg ccccactctg
    14701 atgtggggga gccacctgcc tctactgggg cccagcagcc ttctgcatta gccgccctgc
    14761 agccagcaaa ggagcggcgg agttcctcct cgtcgtcgtc gtcctctagc tcctcctctt
    14821 cttcatcatc gtcgtcgtcg tcctcctcct cctctggctc cagttctagt gactcagagg
    14881 gctctagcct tcctgtgcaa cctgaggtgg cactgaagag ggtccccagc cccaccccag
    14941 ccccaaagga ggctgttcga gagggacgtc ctccggagcc aaccccagcc aaacggaaga
    15001 ggcgctctag cagttccagt tccagctcct cctcttcatc ttcctcctcc tcctcctcct
    15061 cctcttcttc ctcctcctct tcctcttctt cttcttcctc ctcatcttcc tcctcctcgt
    15121 cgtcttcctc cccttcccct gctaagcctg gccctcaggc cttgcccaaa cctgcaagcc
    15181 ccaagaagcc accccctggc gagcggaggt cccgcagccc ccggaagcca atagactccc
    15241 tcagggactc tcggtccctc agctactcgc ctgtggagcg tcgccgtccc tcgccccagc
    15301 cctcaccacg ggaccagcag agcagcagca gtgagcgggg ttcccggaga ggccagcgtg
    15361 gggacagccg ctcccccagc cacaagcgca ggagggagac acctagccct cggcccatga
    15421 gacaccgctc ctccaggtct ccattaccgg tcgccaccat ggtgagcaag ggcgaggagg
    15481 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
    15541 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
    15601 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
    15661 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
    15721 tgaagctgtc cttccccgag ggcttcaatt gggagcgcgt gatgaacttc gaggacggcg
    15781 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
    15841 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagtgtcgt accatgggct
    15901 gggaggcctc cactgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
    15961 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
    16021 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcga catcaagttg gacatccttt
    16081 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
    16141 ccggcggcat ggacgagctg tacaagtaag aattctaccg ggtaggggag gcgcttttcc
    16201 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
    16261 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
    16321 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
    16381 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
    16441 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
    16501 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
    16561 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
    16621 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
    16681 cctgcagccc aagcttacc
//
