LOCUS       sequence-342305-       10197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab 
            clone DPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, 
            Hall TE
  TITLE     In vivo proteomic mapping through GFP-directed proximity-dependent 
            biotin labelling in zebrafish.
  JOURNAL   Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631.
  PUBMED    33591275
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-16"; volume: "10. pii: 64631. doi"; pages: " 
            10.7554/eLife.64631"
COMMENT     Sequence Label: acta1-Cavin1a-Clover
FEATURES             Location/Qualifiers
     source          1..10197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5074..5098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5099..5108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6351..6375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6377..7093
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6419..6440)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(7094..7114)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7122..7138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7154..7288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7178..7197)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7232..7251
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7727..7914,7915..7926)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7960..7976)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7960..7976)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8013..8033)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8013..8031)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8052..8068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8065..8087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8076..8092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8100..8106,8107..8124,8125..8130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8145..8166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8283..8300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8454..9042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8534..8553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9213..10004,10005..10073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9836..9855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10074..10178)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     1321 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     1381 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     1441 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     1501 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     1561 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     1621 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     1681 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     1741 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     1801 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     1861 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     1921 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     1981 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     2041 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     2101 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     2161 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     2221 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     2281 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     2341 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     2401 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     2461 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     2521 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     2581 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     2641 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     2701 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     2761 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     2821 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     2881 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     2941 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     3001 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     3061 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     3121 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
     3181 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
     3241 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
     3301 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
     3361 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
     3421 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
     3481 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     3541 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     3601 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     3661 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
     3721 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
     3781 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
     3841 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
     3901 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
     3961 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
     4021 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
     4081 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
     4141 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
     4201 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
     4261 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
     4321 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
     4381 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
     4441 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
     4501 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
     4561 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
     4621 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
     4681 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
     4741 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
     4801 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
     4861 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
     4921 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
     4981 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
     5041 tgcgctgttg tgattggtcc tgcacagacc aaggcaagtt tgtacaaaaa agcaggctgc
     5101 caccatggcc gacacagact tcaaactgga gcgtgccatc ttcgccgaag tctctgatga
     5161 cgatgaggag gtggctttgg ttacatctgc agccaaacct gcaacatcct tgaaggagtt
     5221 caaatcgaga cgaggagacg atgaggatga tgatgatgac tcagagaagg aggtggatct
     5281 ggtgttgggt ccagggctgg acggtggtga cgcagagaag tcggatgcta ctggcatatt
     5341 ggtgctggcg cttctagata aaatcatcgg tgtggtggac aagatccagc aaacgcagac
     5401 gagcctggag gccaagcagg aggtgatgga gaagaacatg agcagcattc agacggagtt
     5461 ggccaagttg gccaagagtc acgtgggcac ggccgggacg gtcaacaagc tcctggacaa
     5521 ggtgcggaag gtaaacgtca acgtgaagag cgtgcgagcg gacttggaga agcaagtggg
     5581 acagatcaaa aaactagaga acaacgaaca ggagcttctc aagcggaaga acatcaaagt
     5641 gctcattata ccggaaaaag ggaaacctgt gaaagtctct aagaagcctg caggagaaga
     5701 aggagagcaa gtgctaagtg aaggaggaga ggaagagcat gcctttgaag gagaagaagt
     5761 ggaggtcgag gagaccattg aggagtcccg agccgaacgc atcaagcgca gcggcctcca
     5821 gaaggtggat gagctgaaga aagccttcag caaggagcag atgcagaaga ccagacagaa
     5881 aaccaaagag aacctggaga aaacccgaat gagaaccaaa gagaacctgg agaagacccg
     5941 acagaggacc agagagaacc tggagaagac aaagaaaagt ctaggcaaga agatggggaa
     6001 gttcggaaca aaactgaccc caaacaccga gcgcagagaa aagatccgca gctccaggga
     6061 gaagatgaag aagtcgctga accccgacca caccgtctac gcccgctcca aatccaccac
     6121 ctatcgcgtg ccgccgttca ccttccacgt taagaagatc cgcgggggcg aggcggagcc
     6181 cacgccagag ccggaggagg aagatgagga gcagaaggaa gaggaggagg aaggagatct
     6241 cgtggaagaa gctgaactgg tcagtctgga tggcccagag atggaggcgg cagatcgctc
     6301 tagactggcc ttccaggaga cggtgaggcc tcgagagacg gccggcctgg acccagcttt
     6361 cttgtacaaa gtggtggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6421 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     6481 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     6541 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     6601 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6661 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     6721 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6781 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
     6841 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     6901 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6961 gcccgacaac cactacctga gccatcagtc cgccctgagc aaagacccca acgagaagcg
     7021 cgatcacatg gtcctgctgg agttcgtaac cgccgccggg atcactctcg gcatggacga
     7081 gctgtacaag taacaacttt attatacata gttgataatt cactggccgt cgttttacgg
     7141 taccatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
     7201 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7261 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     7321 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     7381 ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc
     7441 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
     7501 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
     7561 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
     7621 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
     7681 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
     7741 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
     7801 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
     7861 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
     7921 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
     7981 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     8041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga
     9121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
