LOCUS       sequence-342306-        9939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish muscle specific expression of Cavin4a-Clover, Parton lab 
            clone DPV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, 
            Hall TE
  TITLE     In vivo proteomic mapping through GFP-directed proximity-dependent 
            biotin labelling in zebrafish.
  JOURNAL   Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631.
  PUBMED    33591275
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-16"; volume: "10. pii: 64631. doi"; pages: " 
            10.7554/eLife.64631"
COMMENT     Sequence Label: acta1-Cavin4a-Clover
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5074..5098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5099..5108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6093..6117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6119..6835
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6161..6182)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(6836..6856)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6864..6880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6896..7030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6920..6939)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6974..6993
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7469..7656,7657..7668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7702..7718)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7702..7718)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7755..7775)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7755..7773)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7794..7810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7807..7829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7818..7834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7842..7848,7849..7866,7867..7872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8025..8042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8196..8784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8276..8295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8955..9746,9747..9815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9578..9597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9816..9920)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     1321 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     1381 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     1441 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     1501 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     1561 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     1621 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     1681 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     1741 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     1801 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     1861 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     1921 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     1981 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     2041 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     2101 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     2161 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     2221 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     2281 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     2341 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     2401 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     2461 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     2521 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     2581 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     2641 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     2701 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     2761 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     2821 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     2881 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     2941 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     3001 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     3061 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     3121 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
     3181 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
     3241 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
     3301 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
     3361 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
     3421 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
     3481 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     3541 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     3601 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     3661 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
     3721 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
     3781 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
     3841 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
     3901 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
     3961 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
     4021 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
     4081 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
     4141 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
     4201 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
     4261 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
     4321 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
     4381 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
     4441 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
     4501 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
     4561 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
     4621 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
     4681 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
     4741 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
     4801 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
     4861 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
     4921 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
     4981 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
     5041 tgcgctgttg tgattggtcc tgcacagacc aaggcaagtt tgtacaaaaa agcaggctgc
     5101 caccatggaa aagagaggag atgtcatatt aggggttgag gatgaatccg ggcagcccgt
     5161 ctctgccctg tccattctgt ctctgctgga gcgtgtgtca acaatcatcg atggtgtcca
     5221 ggcaagccaa cagcgtatgg aggaacgcca acagcagctc gagggctcgg tcagcgctgt
     5281 ccagtccgag cttctcaaac tggctcgtga ccacggggcc acagcgacga ccgtcgacaa
     5341 actgctgcag aaggcccggc gtgtgagcac acacgttaaa gaagtacgtt cacgtgtgga
     5401 gaaacagaac gtgcgcgtga agaaagtgga aactacgcaa gatgagctgc tcacacgcaa
     5461 caagttcagg gttgtgatct atcagggtga aaaagaagtc ccctctgttg ctgtcaccaa
     5521 gactcccaag ggagcagggc ttgctgagtt ggaggtggag ccagatgagt atgacatccc
     5581 tgctgacctg tcatctgatg aagagtacat ggtggtggag gatgcggagt cgtccagggg
     5641 cgcccgcctc aaacagtccg ggttaaaagg catcgagaac atcaaggctg cgttctccaa
     5701 agagaacatg aacaagaccc gcgagaagac ccgagaaaac ctcagcaaga ccaaggagag
     5761 cctgagtaag accggccaga cgcttggaac caaattcaac actttgggtg agaagatcgt
     5821 gccacccgag cagcgagaga agataaagca gagcagcgag aggctgaagg aaaacatcgc
     5881 taagaaagcg cccaccaagg aatccttcaa aatcaagctg aagaaggagc gcacggtcgc
     5941 tgaaggccag gagggtgcgg aggcggaacc tgctgtgact ccacccaaag gcaggaagtc
     6001 cagcccagat gtgacctaca cagaggtggt taccgaaaac aaaagggagg gtccagtatc
     6061 agaggaggga gcaactcgta tccatgagga cgacccagct ttcttgtaca aagtggtggt
     6121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     6181 cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa
     6241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     6301 gaccaccttc ggctacggcg tggcctgctt cagccgctac cccgaccaca tgaagcagca
     6361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa
     6421 ggacgacggt acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     6481 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6541 ggagtacaac ttcaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat
     6601 caaggctaac ttcaagatcc gccacaacgt tgaggacggc agcgtgcagc tcgccgacca
     6661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6721 gagccatcag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6781 ggagttcgta accgccgccg ggatcactct cggcatggac gagctgtaca agtaacaact
     6841 ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc
     6901 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6961 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7021 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7081 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     7141 agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     7201 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     7261 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     7321 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     7381 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     7441 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     7501 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     7561 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     7621 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     7681 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     7741 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     7801 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7861 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7921 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7981 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8041 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8821 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     8881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8941 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9001 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9061 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9121 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9181 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9241 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9301 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9361 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9421 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9481 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9541 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9601 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9661 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9721 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9781 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9841 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9901 aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
