LOCUS       sequence-342307-        9906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab 
            clone DQZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, 
            Hall TE
  TITLE     In vivo proteomic mapping through GFP-directed proximity-dependent 
            biotin labelling in zebrafish.
  JOURNAL   Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631.
  PUBMED    33591275
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-16"; volume: "10. pii: 64631. doi"; pages: " 
            10.7554/eLife.64631"
COMMENT     Sequence Label: acta1-Cavin4b-Clover
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5074..5098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5099..5108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6060..6084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6086..6802
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6128..6149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(6803..6823)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6831..6847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6863..6997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6887..6906)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6941..6960
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7436..7623,7624..7635)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7669..7685)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7669..7685)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7722..7742)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7722..7740)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7761..7777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7774..7796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7785..7801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7809..7815,7816..7833,7834..7839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7854..7875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7992..8009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8163..8751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8243..8262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8922..9713,9714..9782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9545..9564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9783..9887)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     1321 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     1381 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     1441 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     1501 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     1561 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     1621 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     1681 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     1741 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     1801 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     1861 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     1921 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     1981 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     2041 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     2101 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     2161 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     2221 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     2281 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     2341 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     2401 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     2461 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     2521 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     2581 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     2641 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     2701 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     2761 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     2821 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     2881 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     2941 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     3001 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     3061 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     3121 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
     3181 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
     3241 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
     3301 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
     3361 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
     3421 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
     3481 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     3541 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     3601 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     3661 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
     3721 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
     3781 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
     3841 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
     3901 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
     3961 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
     4021 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
     4081 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
     4141 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
     4201 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
     4261 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
     4321 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
     4381 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
     4441 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
     4501 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
     4561 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
     4621 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
     4681 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
     4741 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
     4801 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
     4861 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
     4921 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
     4981 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
     5041 tgcgctgttg tgattggtcc tgcacagacc aaggcaagtt tgtacaaaaa agcaggctgc
     5101 caccatggct gataaactgg gactggcagg tgtggcggac gagcccagcg ccatgaacat
     5161 cttctctctg ctggagaggg tgtccgggat catagacaat gttcaggcct gccagcagcg
     5221 gatggaggag aggcagctgg agctggagaa caccgtcaag accatccaag cagatgtagt
     5281 caagctgacg aaggagcaca cggcgaccag tggcaccgtg gagaaacttc tggagaaaac
     5341 acgcaaggtc agctgccaca tcaaggacgt ccgcgtgcgg gtggagaagc agaacgttcg
     5401 agtcaagaag gtggaggaaa cccaagtcga gctgctgaat aagaacaagt tccgtgtcgt
     5461 catctaccag ggagataatg aggtcccagc tgtggctggt cctaaagcct ctccgaaggg
     5521 agcaatgggt ggagacgctg ctgcagaccc agatgcacca gaggctccgc tccctgactc
     5581 ggatgaggaa tacatggttg ttgaagaagc cgattcttcc actgctgcca aactaaagaa
     5641 gaccggcctg aagcgcatcg aaagcctgaa gcagaccttc tcacgcgaga acatgactaa
     5701 gaccaaagaa aacctgggca ccaaggtcaa caagctgggc gagcgcatag taacagccga
     5761 acggcgtgag aaaatccgcc agtcaggaga acgactgaaa cagtctggcg agcgcttcaa
     5821 ggaaaccatc acaaaaaccg taccagcaaa gctgaatctg aagaaggaaa ggacggtggc
     5881 tgagggtcag gagggggcag aagggaccac agaaggaaca gcacccgtcc caccaccgaa
     5941 gggtcgtaaa cccagtcctg aggtggccta cacagagcca acgcagaaac aagaaggtcc
     6001 agatgagggc aagggtgagg aatcagaagt accgatgtat gatatgaagc agctttctga
     6061 cccagctttc ttgtacaaag tggtggtgag caagggcgag gagctgttca ccggggtggt
     6121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga
     6181 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6241 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag
     6301 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6361 cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt
     6421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6481 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat
     6541 cacggccgac aagcagaaga acggcatcaa ggctaacttc aagatccgcc acaacgttga
     6601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6661 cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca aagaccccaa
     6721 cgagaagcgc gatcacatgg tcctgctgga gttcgtaacc gccgccggga tcactctcgg
     6781 catggacgag ctgtacaagt aacaacttta ttatacatag ttgataattc actggccgtc
     6841 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     6901 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6961 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7021 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     7081 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     7141 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     7201 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     7261 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     7321 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     7381 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     7441 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     7501 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     7561 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     7621 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     7681 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     7741 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7801 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7861 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7921 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8041 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8101 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8581 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8761 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     8821 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9061 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9901 aaagtg
//
