LOCUS       sequence-342313-        3633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  membrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Wurz AI, Zheng KS, Hughes RM
  TITLE     Optogenetic Regulation of EphA1 RTK Activation and Signaling.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139.
  PUBMED    38370612
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139."
COMMENT     Sequence Label: pm-EphA1-SAM-Cry2olig
FEATURES             Location/Qualifiers
     source          1..3633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1405..2898
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     regulatory      2917..2926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2923..3633
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3068..3086)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3336..3355
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggcagca gcaaaagcaa accgaaaggc ggcagcatgg tgtccagggg cctgactgga
       61 ggagagattg tagccgtcat ctttgggctg ctgcttggtg cagccttgct gcttgggatt
      121 ctcgttttcc ggtccaggag agcccagcgg cagaggcagc agaggcagcg tgaccgcgcc
      181 accgatgtgg atcgagagga caagctgtgg ctgaagcctt atgtggacct ccaggcatac
      241 gaggaccctg cacagggagc cttggacttt acccgggagc ttgatccagc gtggctgatg
      301 gtggacactg tcataggaga aggagagttt ggggaagtgt atcgagggac cctgaggctc
      361 cccagccagg actgcaagac tgtggccatt aagaccttaa aagacacatc cccaggtggc
      421 cagtggtgga acttccttcg agaggcaact atcatgggcc agtttagcca cccgcatatt
      481 ctgcatctgg aaggcgtcgt cacaaagcga aagccgatca tgatcatcac agaatttatg
      541 gagaatggag ccctggatgc cttcctgagg gagcgggagg accagctggt ccctgggcag
      601 ctagtggcca tgctgcaggg catagcatct ggcatgaact acctcagtaa tcacaattat
      661 gtccaccggg acctggctgc cagaaacatc ttggtgaatc aaaacctgtg ctgcaaggtg
      721 tctgactttg gcctgactcg cctcctggat gactttgatg gcacatacga aacccaggga
      781 ggaaagatcc ctatccgttg gacagcccct gaagccattg cccatcggat cttcaccaca
      841 gccagcgatg tgtggagctt tgggattgtg atgtgggagg tgctgagctt tggggacaag
      901 ccttatgggg agatgagcaa tcaggaggtt atgaagagca ttgaggatgg gtaccggttg
      961 ccccctcctg tggactgccc tgcccctctg tatgagctca tgaagaactg ctgggcatat
     1021 gaccgtgccc gccggccaca cttccagaag cttcaggcac atctggagca actgcttgcc
     1081 aacccccact ccctgcggac cattgccaac tttgacccca gggtgactct tcgcctgccc
     1141 agcctgagtg gctcagatgg gatcccgtat cgaaccgtct ctgagtggct cgagtccata
     1201 cgcatgaaac gctacatcct gcacttccac tcggctgggc tggacaccat ggagtgtgtg
     1261 ctggagctga ccgctgagga cctgacgcag atgggaatca cactgcccgg gcaccagaag
     1321 cgcattcttt gcagtattca gggattcaag gacttgggcg gcagcgactc gctagcgcta
     1381 ccggactcag atctcgaggc caccatgaag atggacaaaa agactatagt ttggtttaga
     1441 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
     1501 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
     1561 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
     1621 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
     1681 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
     1741 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     1801 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     1861 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     1921 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     1981 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     2041 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     2101 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     2161 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     2221 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     2281 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     2341 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     2401 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     2461 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     2521 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     2581 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     2641 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     2701 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     2761 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     2821 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tggagcacag
     2881 atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     2941 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3001 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3061 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3121 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3181 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3241 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3301 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3361 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     3421 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3481 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     3541 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     3601 tccaccggcg gcatggacga gctgtacaag taa
//
