LOCUS       sequence-342316-        3234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  membrane localized EphrinA1 RTK fusion to Cry2olig - RTK domain 
            only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Wurz AI, Zheng KS, Hughes RM
  TITLE     Optogenetic Regulation of EphA1 RTK Activation and Signaling.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139.
  PUBMED    38370612
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139."
COMMENT     Sequence Label: pm-EphA1-RTK-Cry2olig
FEATURES             Location/Qualifiers
     source          1..3234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1006..2499
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     regulatory      2518..2527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2524..3234
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2669..2687)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2937..2956
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggcagca gcaaaagcaa accgaaaggc ggcagcatgg accctgcaca gggagccttg
       61 gactttaccc gggagcttga tccagcgtgg ctgatggtgg acactgtcat aggagaagga
      121 gagtttgggg aagtgtatcg agggaccctg aggctcccca gccaggactg caagactgtg
      181 gccattaaga ccttaaaaga cacatcccca ggtggccagt ggtggaactt ccttcgagag
      241 gcaactatca tgggccagtt tagccacccg catattctgc atctggaagg cgtcgtcaca
      301 aagcgaaagc cgatcatgat catcacagaa tttatggaga atggagccct ggatgccttc
      361 ctgagggagc gggaggacca gctggtccct gggcagctag tggccatgct gcagggcata
      421 gcatctggca tgaactacct cagtaatcac aattatgtcc accgggacct ggctgccaga
      481 aacatcttgg tgaatcaaaa cctgtgctgc aaggtgtctg actttggcct gactcgcctc
      541 ctggatgact ttgatggcac atacgaaacc cagggaggaa agatccctat ccgttggaca
      601 gcccctgaag ccattgccca tcggatcttc accacagcca gcgatgtgtg gagctttggg
      661 attgtgatgt gggaggtgct gagctttggg gacaagcctt atggggagat gagcaatcag
      721 gaggttatga agagcattga ggatgggtac cggttgcccc ctcctgtgga ctgccctgcc
      781 cctctgtatg agctcatgaa gaactgctgg gcatatgacc gtgcccgccg gccacacttc
      841 cagaagcttc aggcacatct ggagcaactg cttgccaacc cccactccct gcggaccatt
      901 gccaactttg accccagggt gactcttcgc ctgcccagcc tgagtggctc agatgggggc
      961 ggcagcgact cgctagcgct accggactca gatctcgagg ccaccatgaa gatggacaaa
     1021 aagactatag tttggtttag aagagaccta aggattgagg ataatcctgc attagcagca
     1081 gctgctcacg aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag
     1141 ttttatcctg gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa
     1201 tccttgaagg ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg
     1261 atcttggatt gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat
     1321 cctgtttcgt tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct
     1381 gtgcaaagct acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc
     1441 aaacctttta cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc
     1501 gttatgcttc ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg
     1561 gcgtgttcga ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg
     1621 ttaactagag cttggtctcc aggatggagc aatgctgata agttactaaa tgagttcatc
     1681 gagaagcagt tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca
     1741 ctactttctc cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc
     1801 cggatgaaac aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat
     1861 ctttttctta ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg
     1921 tttactcacg agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt
     1981 gataagttca aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg
     2041 agagagcttt gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt
     2101 gctgtgaagt ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt
     2161 ttggatgctg atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc
     2221 gatggccacg agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca
     2281 gaaggtgagt acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc
     2341 catcatccat gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca
     2401 aactatgcga aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt
     2461 tcaagaaccc gtggagcaca gatcatgatc ggagcagcag cccgggatcc accggtcgcc
     2521 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     2581 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     2641 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     2701 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     2761 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2821 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     2881 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     2941 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     3001 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     3061 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     3121 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3181 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaa
//
