LOCUS       sequence-342326-       11255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing all three D-type cyclins (cyclin D1, 
            cyclin D2, cyclin D3) from a single promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R,
            Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, 
            Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, 
            McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, 
            Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
  TITLE     The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
  JOURNAL   Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7.
            Epub 2021 Apr 14.
  PUBMED    33854239
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03474-7"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "794-798"
COMMENT     Sequence Label: pLV-hCCND1-T2A-hCCND2-P2A-hCCND3
FEATURES             Location/Qualifiers
     source          1..11255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3769..3778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4062..4115
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4992..5048
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5928..5952)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5970..6558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6023..6043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6441..6452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6589..7088
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6624..6647)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7021..7040
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7105..7704
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7105..7124)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7601..7621
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7776..8009
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8081..8202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8118..8137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8172..8191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8242..8377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8397..8416)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8398..8416)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8426..8443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8426..8442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8435..8457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8584..9039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8671..8690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8881..8902
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9065..9169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9170..9238,9239..10030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9388..9407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10201..10789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10690..10709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10943..10960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11077..11098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11113..11118,11119..11136,11137..11143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11151..11167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11156..11178
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11175..11191
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11175..11191
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11210..11230
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11212..11230
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaacaccagc
     3181 tcctgtgctg cgaagtggaa accatccgcc gcgcgtaccc cgatgccaac ctcctcaacg
     3241 accgggtgct gcgggccatg ctgaaggcgg aggagacctg cgcgccctcg gtgtcctact
     3301 tcaaatgtgt gcagaaggag gtcctgccgt ccatgcggaa gatcgtcgcc acctggatgc
     3361 tggaggtctg cgaggaacag aagtgcgagg aggaggtctt cccgctggcc atgaactacc
     3421 tggaccgctt cctgtcgctg gagcccgtga aaaagagccg cctgcagctg ctgggggcca
     3481 cttgcatgtt cgtggcctct aagatgaagg agaccatccc cctgacggcc gagaagctgt
     3541 gcatctacac cgacaactcc atccggcccg aggagctgct gcaaatggag ctgctcctgg
     3601 tgaacaagct caagtggaac ctggccgcaa tgaccccgca cgatttcatt gaacacttcc
     3661 tctccaaaat gccagaggcg gaggagaaca aacagatcat ccgcaaacac gcgcagacct
     3721 tcgttgccct ctgtgccaca gatgtgaagt tcatttccaa tccgccctcc atggtggcag
     3781 cggggagcgt ggtggccgca gtgcaaggcc tgaacctgag gagccccaac aacttcctgt
     3841 cctactaccg cctcacacgc ttcctctcca gagtgatcaa gtgtgacccg gactgcctcc
     3901 gggcctgcca ggagcagatc gaagccctgc tggagtcaag cctgcgccag gcccagcaga
     3961 acatggaccc caaggccgcc gaggaggagg aagaggagga ggaggaggtg gacctggctt
     4021 gcacacccac cgacgtgcgg gacgtggaca tcggaagcgg agagggcagg ggaagtcttc
     4081 taacatgcgg ggacgtggag gaaaatcccg gccccatgga gctgctgtgc cacgaggtgg
     4141 acccggtccg cagggccgtg cgggaccgca acctgctccg agacgaccgc gtcctgcaga
     4201 acctgctcac catcgaggag cgctaccttc cgcagtgctc ctacttcaag tgcgtgcaga
     4261 aggacatcca accctacatg cgcagaatgg tggccacctg gatgctggag gtctgtgagg
     4321 aacagaagtg cgaagaagag gtcttccctc tggccatgaa ttacctggac cgtttcttgg
     4381 ctggggtccc gactccgaag tcccatctgc aactcctggg tgctgtctgc atgttcctgg
     4441 cctccaaact caaagagacc agcccgctga ccgcggagaa gctgtgcatt tacaccgaca
     4501 actccatcaa gcctcaggag ctgctggagt gggaactggt ggtgctgggg aagttgaagt
     4561 ggaacctggc agctgtcact cctcatgact tcattgagca catcttgcgc aagctgcccc
     4621 agcagcggga gaagctgtct ctgatccgca agcatgctca gaccttcatt gctctgtgtg
     4681 ccaccgactt taagtttgcc atgtacccac cgtcgatgat cgcaactgga agtgtgggag
     4741 cagccatctg tgggctccag caggatgagg aagtgagctc gctcacttgt gatgccctga
     4801 ctgagctgct ggctaagatc accaacacag acgtggattg tctcaaagct tgccaggagc
     4861 agattgaggc ggtgctcctc aatagcctgc agcagtaccg tcaggaccaa cgtgacggat
     4921 ccaagtcgga ggatgaactg gaccaagcca gcacccctac agacgtgcgg gatatcgacc
     4981 tgggaagcgg agccacgaac ttctctctgt taaagcaagc aggagatgtt gaagaaaacc
     5041 ccgggcctat ggagctgctg tgttgcgaag gcacccggca cgcgccccgg gccgggccgg
     5101 acccgcggct gctgggggac cagcgtgtcc tgcagagcct gctccgcctg gaggagcgct
     5161 acgtaccccg cgcctcctac ttccagtgcg tgcagcggga gatcaagccg cacatgcgga
     5221 agatgctggc ttactggatg ctggaggtat gtgaggagca gcgctgtgag gaggaagtct
     5281 tccccctggc catgaactac ctggatcgct acctgtcttg cgtccccacc cgaaaggcgc
     5341 agttgcagct cctgggtgcg gtctgcatgc tgctggcctc caagctgcgc gagaccacgc
     5401 ccctgaccat cgaaaaactg tgcatctaca ccgaccacgc tgtctctccc cgccagttgc
     5461 gggactggga ggtgctggtc ctagggaagc tcaagtggga cctggctgct gtgattgcac
     5521 atgatttcct ggccttcatt ctgcaccggc tctctctgcc ccgtgaccga caggccttgg
     5581 tcaaaaagca tgcccagacc tttttggccc tctgtgctac agattatacc tttgccatgt
     5641 acccgccatc catgatcgcc acgggcagca ttggggctgc agtgcaaggc ctgggtgcct
     5701 gctccatgtc cggggatgag ctcacagagc tgctggcagg gatcactggc actgaagtgg
     5761 actgcctgcg ggcctgtcag gagcagatcg aagctgcact cagggagagc ctcagggaag
     5821 cctctcagac cagctccagc ccagcgccca aagccccccg gggctccagc agccaagggc
     5881 ccagccagac cagcactcct acagatgtca cagccataca cctgtagacc cagctttctt
     5941 gtacaaagtg gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa
     6001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6541 tttgggccgc ctccccgcat cgggaattcc cgcggttcga attctaccgg gtaggggagg
     6601 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     6661 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     6721 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     6781 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     6841 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     6901 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     6961 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     7021 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     7081 gcctttcgac ctcacgtggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7141 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7201 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7261 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7321 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7381 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7441 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7501 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7561 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7621 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7681 atgacccgca agcccggtgc ctgaggtacc tttaagacca atgacttaca aggcagctgt
     7741 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7801 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7981 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8041 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8221 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8341 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     8401 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8461 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8521 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8581 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8641 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8701 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8761 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8821 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8881 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8941 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9001 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     9061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga
    10621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    10981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    11041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    11101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    11161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    11221 tcactaaagg gaacaaaagc tggagctgca agctt
//
