LOCUS       sequence-342376-        4041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC19A3_STOP
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1598..1697)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1714..1733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1715..1733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1738..1754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1738..1754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1856..1875)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1867..2676
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1934..1953)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2823..3411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3312..3331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3565..3582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3741..3768)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3860..3946)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3995..4017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4009..4026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4010..4026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ctgctacagg
      121 accagcctga gctcctcttg gatctatcct accgtgatcc tgtgcctgtt tggcttcttt
      181 agcatgatgc gcccttccga gccattcctg atcccctacc tgtccggccc tgacaagaac
      241 ctgacctctg ccgagatcac aaatgagatc tttcccgtgt ggacatactc ttatctggtg
      301 ctgctgctgc ccgtgttcgt gctgaccgat tacgtgcggt ataagccagt gatcatcctg
      361 cagggcatct cctttatcat cacctggctg ctgctgctgt tcggacaggg agtgaagaca
      421 atgcaggtgg tggagttctt ttacggaatg gtgaccgcag cagaggtggc atactatgcc
      481 tacatctatt ccgtggtgtc tccagagcac taccagcggg tgtctggcta ttgcagaagc
      541 gtgaccctgg cagcatacac agcaggaagc gtgctggcac agctgctggt gtcactcgcg
      601 aacatgagtt acttctatct gaatgtgatc tccctggcca gcgtgtccgt ggccttcctg
      661 ttttctctgt tcctgcccat gcctaagaag agcatgttct ttcacgccaa gccctcccgc
      721 gagatcaaga agagctcctc tgtgaaccct gtgctggagg agacccacga gggagaggca
      781 cctggatgcg aggagcagaa gccaacctcc gagatcctgt ctacaagcgg caagctgaac
      841 aagggccagc tgaattctct gaagccaagc aatgtgacag tggacgtgtt cgtgcagtgg
      901 tttcaggatc tgaaggagtg ttacagctcc aagcggctgt tctattggag cctgtggtgg
      961 gccttcgcca ccgcaggctt taaccaggtg ctgaattacg tgcagatcct gtgggactat
     1021 aaggccccct cccaggattc tagcatctac aacggcgccg tggaggccat cgccaccttc
     1081 ggcggagcag tggcagcctt tgccgtgggc tatgtgaaag tgaattggga cctgctggga
     1141 gagctggccc tggtggtgtt ttctgtggtg aacgccggca gcctgttcct gatgcactac
     1201 acagccaata tctgggcctg ttacgccggc tatctgatct ttaagtcctc ttatatgctg
     1261 ctgatcacca tcgccgtgtt ccagatcgcc gtgaacctga atgtggagcg gtacgccctg
     1321 gtgttcggca tcaacacctt catcgccctg gtcatccaga ccatcatgac agtgatcgtg
     1381 gtggatcaga gaggcctgaa tctgcccgtg agcatccagt tcctggtgta cggcagctat
     1441 tttgccgtga tcgccggcat cttcctgatg aggagcatgt acatcaccta tagcacaaag
     1501 tcccagaagg acgtgcagtc cccagccccc tctgagaacc cagacgtgag ccaccccgag
     1561 gaggagagca atatcatcat gtccaccaag ctgtaagacc cagctttctt gtacaaagtt
     1621 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1681 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1741 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1801 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1861 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1921 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1981 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2041 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2101 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2161 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2221 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2281 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2341 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2401 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2461 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2521 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2581 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2641 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2701 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2761 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3661 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3721 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3781 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3841 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3901 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3961 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4021 gccagtctta agctcgggcc c
//
