LOCUS       sequence-342377-        4044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC19A2_STOP
FEATURES             Location/Qualifiers
     source          1..4044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1601..1700)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1717..1736)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1718..1736)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1741..1757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1741..1757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1859..1878)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1870..2679
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1937..1956)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2826..3414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3315..3334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3568..3585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3744..3771)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3863..3949)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3998..4020
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4012..4029
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4013..4029
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cgtgccaggc
      121 cccgtgtcca ggagagcagc agcagcagca gcaaccgtgc tgctgcggac agccagagtg
      181 aggagggagt gctggttcct gcctaccgcc ctgctgtgcg catacggctt ctttgcctct
      241 ctgcggccca gcgagccttt tctgacccca tatctgctgg gccccgacaa gaacctgaca
      301 gagagagagg tgttcaatga gatctacccc gtgtggacct actcttatct ggtgctgctg
      361 ttccccgtgt tcctggccac agattacctg cggtataagc cagtggtgct gctgcagggc
      421 ctgagcctga tcgtgacctg gtttatgctg ctgtacgccc agggcctgct ggccatccag
      481 ttcctggagt tcttttatgg catcgccacc gccacagaga tcgcctacta ttcctacatc
      541 tattctgtgg tggatctggg catgtaccag aaggtgacca gctattgccg gtccgccacc
      601 ctggtgggct ttacagtggg ctctgtgctg ggacagatcc tggtgagcgt ggcaggatgg
      661 tccctgttct ctctgaacgt gatctccctg acctgcgtgt ctgtggcctt cgccgtggcc
      721 tggtttctgc ctatgccaca gaagtccctg ttctttcacc acatcccatc tacatgtcag
      781 agagtgaacg gcatcaaggt gcagaatggc ggcatcgtga ccgacacacc tgccagcaac
      841 cacctgccag gctgggagga tatcgagtcc aagatccccc tgaatatgga ggagccacct
      901 gtggaggagc cagagcctaa gccagacagg ctgctggtgc tgaaggtgct gtggaatgat
      961 tttctgatgt gctacagctc caggccactg ctgtgctgga gcgtgtggtg ggcactgtcc
     1021 acctgcggct acttccaggt ggtgaactat acacagggcc tgtgggagaa agtgatgcct
     1081 tctagatacg ccgccatcta taatggcggc gtggaggccg tgagcaccct gctgggagca
     1141 gtggccgtgt ttgcagtggg ctacatcaag atctcttgga gcacctgggg cgagatgaca
     1201 ctgagcctgt tctccctgct gatcgcagca gccgtgtata tcatggacac agtgggcaac
     1261 atctgggtgt gctacgccag ctatgtggtg tttaggatca tctacatgct gctgatcacc
     1321 atcgccacat tccagatcgc cgccaacctg tccatggagc ggtacgccct ggtgttcggc
     1381 gtgaatacct ttatcgccct ggccctgcag accctgctga cactgatcgt ggtggacgca
     1441 tccggactgg gactggagat caccacacag ttcctgatct acgcctctta ttttgccctg
     1501 atcgcagtgg tgttcctggc atctggagcc gtgagcgtga tgaagaagtg tcgcaagctg
     1561 gaggatcccc agtctagctc ccaggtgacc acaagctaag acccagcttt cttgtacaaa
     1621 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1681 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1741 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1801 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1861 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1921 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1981 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2041 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2101 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2161 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2221 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2281 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2341 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2401 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2461 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2521 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2581 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2641 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2701 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2761 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2941 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3721 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3781 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3841 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3901 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3961 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4021 acggccagtc ttaagctcgg gccc
//
