LOCUS       sequence-342378-        4182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-MFSD2A_STOP
FEATURES             Location/Qualifiers
     source          1..4182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1739..1838)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1855..1874)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1856..1874)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1879..1895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1879..1895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1997..2016)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2008..2817
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2075..2094)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2964..3552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3453..3472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3706..3723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3882..3909)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4001..4087)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4136..4158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4150..4167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4151..4167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caagggagag
      121 ggagcagaga gcggatccgc cgccggcctg ctgccaacca gcatcctgca gtccacagag
      181 cgccctgccc aggtgaagaa ggagcccaag aagaagaagc agcagctgtc cgtgtgcaac
      241 aagctgtgct acgcactggg aggagcacct tatcaggtga ccggatgcgc cctgggcttc
      301 tttctgcaga tctacctgct ggacgtggcc cagaaggatg aggaggtggt gttctgtttt
      361 agctcctttc aagtgggccc attctctgcc agcatcatcc tgtttgtggg aagggcatgg
      421 gacgcaatca ccgatccact ggtgggactg tgcatctcca agtctccttg gacatgtctg
      481 ggccggctga tgccctggat catcttcagc acccctctgg ccgtgatcgc ctacttcctg
      541 atctggtttg tgcccgactt ccctcacggc cagacatact ggtatctgct gttttactgc
      601 ctgttcgaga ccatggtgac atgttttcac gtgccctatt ccgccctgac catgttcatc
      661 tctaccgagc agacagagcg cgattctgcc accgcctatc ggatgacagt ggaggtgctg
      721 ggaaccgtgc tgggaacagc aatccaggga cagatcgtgg gacaggcaga caccccttgc
      781 ttccaggatc tgaactctag caccgtggcc agccagtccg ccaatcacac acacggcacc
      841 acaagccacc gggagaccca gaaggcatac ctgctggcag caggcgtgat cgtgtgcatc
      901 tatatcatct gtgccgtgat cctgatcctg ggcgtgcgcg agcagaggga gccttacgag
      961 gcccagcagt ctgagccaat cgcctacttc cggggactga ggctggtcat gagccacggc
     1021 ccctacatca agctgatcac cggcttcctg tttacaagcc tggccttcat gctggtggag
     1081 ggcaacttcg tgctgttttg tacctataca ctgggcttta gaaacgagtt ccagaatctg
     1141 ctgctggcca tcatgctgtc cgccaccctg acaatcccaa tctggcagtg gtttctgacc
     1201 aggttcggca agaagacagc cgtgtacgtg ggcatctcct ctgccgtgcc ctttctgatc
     1261 ctggtggccc tgatggagtc caatctgatc atcacctatg ccgtggcagt ggcagcagga
     1321 atcagcgtgg cagcagcctt cctgctgcca tggtccatgc tgcccgacgt gatcgacgat
     1381 tttcacctga agcagccaca cttccacggc acagagccca tcttcttttc cttttacgtg
     1441 ttctttacca agttcgcctc tggcgtgagc ctgggcatct ccacactgtc tctggatttt
     1501 gccggctatc agaccagagg ctgctctcag ccagagaggg tgaagttcac cctgaacatg
     1561 ctggtgacaa tggcccccat cgtgctgatc ctgctgggcc tgctgctgtt caagatgtac
     1621 cctatcgacg aggagcggag aaggcagaat aagaaggccc tgcaggccct gagggacgag
     1681 gcaagctcct ctggctgttc tgagaccgat agcacagagc tggccagcat cctgtaagac
     1741 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1801 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1861 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1921 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1981 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2041 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2101 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2161 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2221 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2281 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2341 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2401 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2461 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2521 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2581 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2641 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2701 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2761 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2821 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2881 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3841 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3901 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3961 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     4021 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     4081 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4141 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
